Method for generating hairy roots by efficiently inducing trifoliate orange by agrobacterium rhizogenes and application of method

Abstract

The invention discloses a method for generating hairy roots by efficiently inducing trifoliate orange by agrobacterium rhizogenes, and application of the method. The method is accessible, simple and convenient to operate, and high in induction frequency of co-transforming hairy roots. Separated hairy roots can self-grow on an MT basic culture medium, the growing condition is easy to control, and experimental observation is convenient. A hairy root system contains a DR5::YFP (Yellow Fluorescent Protein):GUS (Glucuronidase) reporting gene, and a DR5 promoter is inducted to express by auxin, so that distribution and expression modes of endogenous auxin in the growing and developing process of hairy roots can be intuitively observed according to YFP fluorescent and GUS dyeing results, and the problems that a root system growing in soil is inconvenient to observe and take, the growing environment is hard to control and the like in conventional methods of trifoliate orange root system development study are solved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a method for efficiently inducing hairy roots in trifoliate oranges by using Agrobacterium rhizogenes, and also relates to the application of the hairy roots prepared by the method in the research on the development of the root system of trifoliate oranges. Background technique [0002] Citrus is one of the most important fruit tree crops in the world. At present, the research on citrus is mostly focused on the aboveground organs, such as flower bud differentiation, fruit quality formation mechanism, etc., but there are very few reports on the underground root system. The root system is an important organ for fruit trees to absorb water and nutrients, and plays a vital role in the growth and development of the aboveground parts of fruit trees. Because the root system grows in the soil, it is extremely inconvenient to observe and obtain materials, and the isolated root org...

AI Technical Summary

Problems solved by technology

[0008] Another object carrier of the present invention provides a kind of application in root development research of the hairy root induced by A. The promoter is induced by auxin, so the distribution and expression pattern of endogenous auxin during the growth and development of hairy roots can be visually observed according to the results of YFP fluorescence and GUS staining; it overcomes the problems existing in conventional methods for the study of trifoliate root development, such as The root system grows in the soil, it is inconvenient to observe and obtain materials, and the growth environment is difficult to control, etc.

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