Bacterium for degrading herbicides chlorimuron-ethyl and acetochlor and application of bacterium

A technology of chlorsulfuron-methyl and acetochlor, applied in the direction of bacteria, microorganism-based methods, restoration of polluted soil, etc., can solve the problems of aging of branches and stem bases, plant dwarfing, slow plant growth, etc.

Active Publication Date: 2013-10-02
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The extensive use of chemical herbicides has greatly improved the efficiency of agricultural production, but at the same time it has also brought about great production and environmental problems. For example, the phytotoxicity of long-residual herbicides to subsequent crops is becoming more and more serious, which affects the adjustment of agricultural planting industry structure and brings great impact on agricultural production. serious losses in production
Chlorimuron-methyl has the advantages of less dosage, high activity, and good weeding effect, and is deeply loved by farmers. However, chlorimuron-methyl is a long-acting herbicide with a long residu

Method used

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  • Bacterium for degrading herbicides chlorimuron-ethyl and acetochlor and application of bacterium
  • Bacterium for degrading herbicides chlorimuron-ethyl and acetochlor and application of bacterium
  • Bacterium for degrading herbicides chlorimuron-ethyl and acetochlor and application of bacterium

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Example 1. Isolation and identification of Cellulosimerbium sp. LY1114CGMCC No.7776

[0040] 1. Isolation of herbicide-degrading bacteria LY1114

[0041] Add 10 g of soil samples (collected from farmland polluted by the herbicide acetochlor in Shandong Province, China) to 100 mL of enrichment culture solution containing 20 mg / L of chlorimuron-methyl, and culture at 28°C and 180 r / min for 7 days to enrich for chlorimuron-methyl Sulfur-methyl-degrading bacteria. After the first round of enrichment, 10 mL of the enrichment solution was transferred to 100 mL of the enrichment medium containing 40 mg / L of chlorimuron-methyl, and the culture was continued for 7 days for the second round of enrichment. Such continuous enrichment and culture for 5 times, the concentrations of chlorimuron-methyl were 20, 40, 60, 80 and 100 mg / L in turn.

[0042] The screening of degrading bacteria adopts the plate transparent circle method. Add chlorimuron-methyl to inorganic salt solid medium ...

Embodiment 2

[0061] Example 2, Quantitative determination of the ability of Cellulosimerbium sp. LY1114CGMCC No.7776 to degrade the herbicides chlorimuron-methyl and acetochlor

[0062] 1. Preparation of standard curve for the determination of chlorimuron-methyl

[0063] Use methanol to prepare chlorimuron-methyl standard substance (purchased from Fluka Company) into a series of standard solutions with concentrations of 10, 20, 40, 60, 80, and 100 mg / L, and use high performance liquid chromatography (HPLC) to determine different concentrations of chlorimuron-methyl The peak area of ​​the standard was repeated three times. With the concentration of chlorimuron-methyl as the abscissa and the peak area as the ordinate, draw the chlorimuron-methyl standard curve.

[0064] The detection conditions are as follows:

[0065] Detection system: Agilent1100Series. Chromatographic column: C18Diamosil TM Reversed-phase column, 250mm×4.6mm, particle size 5μm. Chromatographic conditions: mobile phas...

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Abstract

The invention discloses a bacterium for degrading herbicides chlorimuron-ethyl and acetochlor and application of the bacterium. The bacterium is cellulosimicrobium sp. LY114, which is preserved in China General Microbiological Culture Collection Center by a preservation number of CGMCC No.7776. The cellulosimicrobium sp. LY114 CGMCC No.7776 reaches a degrading rate of 89.11% on chlorimuron-ethyl (45mg/L) and acetochlor (45mg/L) in an organic salt culture medium within 7d, showing that the strain can effectively degrade the chlorimuron-ethyl and the acetochlor; and the bacterium has a wide application prospect in an aspect of repairing chlorimuron-ethyl and acetochlor pollution of soil.

Description

technical field [0001] The invention relates to a bacterium for degrading the herbicides chlorimuron-methyl and acetochlor and its application. Background technique [0002] Chlorimuron-methyl, English common name chlorimuron-ethyl, chemical name 2-(4-chloro-6-methoxypyrimidin-2-ylcarbamoylsulfamoyl) methyl benzoate, structural formula as shown in formula 1: [0003] [0004] Chlorimuron-methyl is an ultra-efficient sulfonylurea herbicide, and its target is ALS (acetolactate synthase) in plants, which inhibits the biosynthesis of branched-chain amino acids valine and isoleucine, resulting in the substrate α- The accumulation of butanone hinders the DNA synthesis in interphase of cell division, stops mitosis, and the cells cannot grow normally. Chlorimuron-methyl can be absorbed by the roots, stems, and leaves of plants, and conducts up and down in the plant body. It is a selective pre-emergence and post-emergence herbicide. It is mainly used in farmland such as soybeans ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/01A62D101/04A62D101/28A62D101/26
Inventor 孙建光辛访华高淼周义清
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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