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Bio-functionalized gold nano fluorescent probe and preparation method thereof

A technology of biofunctionalization and fluorescent probes, which is applied in the fields of biomedical engineering and nanomedicine to achieve the effects of simple preparation and detection process, high sensitivity detection and high sensitivity

Inactive Publication Date: 2013-10-09
XIAMEN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Under normal circumstances, gold nanoparticles and proteins form a strong combination due to electrostatic adsorption, and the adsorption will not denature biomolecules. Due to the high electron density of gold particles, dark brown can be seen under the microscope at the gold-labeled protein binding site. Particles, when these markers accumulate in large quantities at the corresponding ligands, red or pink spots are visible to the naked eye, so they are used in qualitative or semi-quantitative rapid immunoassay methods, but quantitative detection and sensitivity need to be improved

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  • Bio-functionalized gold nano fluorescent probe and preparation method thereof
  • Bio-functionalized gold nano fluorescent probe and preparation method thereof
  • Bio-functionalized gold nano fluorescent probe and preparation method thereof

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preparation example

[0039] 1. Preparation example: the chemical substances used in the following steps are commercially available.

[0040] The preparation of gold nano fluorescent probe and the detection method used for biomarkers comprise the following steps:

[0041] 1) Add 20 μL K to 1 mL citrate-gold nanoparticle solution 2 CO 3 solution (100mM) such that the pH of the solution was adjusted to 9.0.

[0042]2) Add 1 μL rhodamine B isothiocyanate fluorescent dye (10 mM) to the mixture under vigorous stirring. After reacting for 1 h, the resulting solution was centrifuged (14,000 rpm, 15 min) to remove excess fluorescent dye, and the nanocomposites were redispersed in 1 mL K 2 CO 3 solution (pH9.0).

[0043] 3) Add antibody (2-antibody) or nucleic acid aptamer (10 μL, 1 mg / mL) to the gold nanoparticle solution enriched with fluorescent dye on the surface, and shake gently for two hours at room temperature.

[0044] 4) The antibody (antibody 2)-fluorochrome-gold nanoparticle complex soluti...

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Abstract

The invention discloses a bio-functionalized gold nano fluorescent probe and a preparation method thereof. The probe is characterized by containing detection antibodies or nucleic acid aptamers, and gold nanoparticles modified on surfaces of fluorescence dyes. The gold nano fluorescent probe can highly-efficiently identify disease biomarkers. The fluorescence dyes are in a quenched state. When mercapto group-containing compounds are added into a detection system, the fluorescent dyes highly-efficiently enriched on the surfaces of the gold nanoparticles are detached, thereby causing substantial activation of the fluorescent probe; and a fluorescent intensity is proportional to a concentration of the biomarkers in samples. The method provided by the invention greatly increases a detection sensitivity of the disease biomarkers, and simultaneously has advantages of simple equipment, easy operation and batch testing.

Description

technical field [0001] The invention relates to a biofunctionalized gold nanometer fluorescent probe. The preparation method and nanomaterials thereof are used for the detection and application of disease biomarkers such as tumors, and belong to the fields of biomedical engineering and nanomedicine. Background technique [0002] In terms of in vitro diagnosis of diseases, the detection technology with high specificity, high sensitivity and high throughput characteristics can not only effectively simplify the process of lesion sample preparation, avoid the use of toxic reagents and the interference of reaction inhibitors, but also the establishment of rapid detection methods, The operation process is simplified, the detection cost is saved, and the timeliness and accuracy of the detection are improved. The current gold standard for clinical biomarker detection is ELISA. Due to its moderate sensitivity, it can only be detected after the level of biomarkers has reached the cri...

Claims

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Application Information

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IPC IPC(8): C09K11/06G01N21/64G01N33/574
Inventor 刘刚陈小元王占通朱雷薛云新
Owner XIAMEN UNIV
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