Method for processing benzoic acid, sorbic acid and saccharin sodium salt in food before detection

Abstract

The invention belongs to the technical field of food detection, and particularly relates to a method for processing benzoic acid, sorbic acid and saccharin sodium salt in food before detection. The method comprises the following steps of 1. smashing: smashing solid samples and semisolid samples into small lumps, and directly taking liquid samples; 2. weighing: weighing a certain amount of samples to be detected in a dialysis bag with the lower end closed; 3. dialyzing: adding sodium hydroxide water solution into the dialysis bag with samples, then tightening the upper end of the dialysis bag, putting into a measuring cylinder and metering the volume with water, standing and dialyzing; and 4. filtering: taking a certain amount of liquid outside the dialysis bag, regulating the pH value to be at acidity, metering the volume with water, then filtering with a filter membrane, and taking the filtrate for detecting. The method has the characteristics of eliminating interferences, protecting a chromatographic column, using less chemical reagent, being safe and reliable, lo3wering the detection cost, being simple in operation, and the like.

Description

technical field [0001] The invention belongs to the technical field of food detection, and specifically relates to a pretreatment method before detecting benzoic acid, sorbic acid and sodium saccharin in food by high performance liquid chromatography, which can effectively remove viscous liquid samples, semi- Interference from proteins, starches, polysaccharides, colloids, granular impurities, bacteria and other substances in solid samples and solid powder samples. Background technique [0002] "Food is the most important thing for the people", food is the material basis for human beings to survive, and it is the first need for human beings to maintain growth and health. Food safety has attracted more and more attention, and the development of food testing technology is the technical support to ensure food safety. [0003] The complete food testing process includes sample collection, sample pretreatment, analysis and determination, data processing, and result expression....

AI Technical Summary

Problems solved by technology

[0006] However, in the actual operation of food testing, there are still many irregular samples, including viscous liquid samples such as sauces, sesame paste, etc., semi-solid samples such as hot pot seasoning, pickled vegetables, etc., and solid samples such as beef powder and barbecue marinade. etc., due to containing thousands of chemical components with different properties and states, the above methods of adjusting the pH value, combining potassium ferrocyanide and zinc acetate to precipitate protein, and extracting and extracting cannot effectively remove protein, fat, Starch and other substances, and the final microporous membrane filtration method can only remove large impurity particles, so it brings great interference to the detection results of the target substance, and seriously pollutes the detection chromatographic column, resulting in a short service life of the chromatographic column

[0007] There is no report on the application of dialysis to high performance liquid chromatography to detect benzoic acid, sorbic acid and sodium saccharin in food pretreatment technology