Photonic crystal microsphere liquid chip chemiluminescence method for multiple detection of mycotoxins with high sensitivity
A photonic crystal microsphere, chemiluminescence technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, measurement devices, etc., can solve the problems of expensive detection instruments, large amount of reagents, and high detection costs, Achieve the effect of saving reagents and samples, low cost and stable detection
Inactive Publication Date: 2015-08-05
NANJING NORMAL UNIVERSITY
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Problems solved by technology
And with the increasing economic exchanges among countries, traditional detection methods have gradually shown shortcomings, such as high detection cost, large amount of reagents, expensive detection equipment, long detection time and other shortcomings
Method used
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Embodiment 1
[0045] Rice, corn and wheat are pulverized with a traditional Chinese medicine grinder, passed through a 200-mesh sieve, weighed 5g of each sample in a 100mL conical flask, added different concentrations and volumes of mycotoxin standard substances to the sample, mixed the sample thoroughly, and Keep it in a ventilated place until the solvent evaporates completely. Add 15mL of extractant (methanol:water=4:6) to it, extract in a shaker at 150r / min for 2 hours, filter with whatman filter paper, and the filtrate is the sample to be tested. At the same time, extract the control sample without adding standard substance according to the above method.
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Abstract
The invention relates to a method for detecting fungaltoxin by using a photonic crystal micro-sphere liquid-phase chip chemiluminiscence method. According to the method, photonic crystal micro-spheres are used as probe carriers of multiple fungaltoxins, the fungaltoxins are detected by carrying out competitive immunodetection on the surfaces of the micro-spheres, the multiple fungaltoxins are competed with antigens fixed on the surfaces of the micro-spheres so as to be combined with respective anti-bodies of the fungaltoxins, second antibodies marked by using horse radish peroxidases are specifically combined with respective fungaltoxin antibody, reflection peaks of the photonic crystal micro-spheres are measured by using a fiber optic spectrometer so as to decode the micro-spheres, and high throughput tests are carried out on chemiluminiscence signals by using a multi-functional microplate reader. The linear detection ranges of the method to aflatoxin, fumonisins and ochratoxin are respectively 0.0001-1ng / mL, 0.0001-1ng / mL and within 0.001-1ng / mL, and only 2mu L is needed for a sample.
Description
technical field [0001] The invention designs a rapid, high-throughput and low-cost detection method for mycotoxins in agricultural products, feed and food. Specifically, the typical aflatoxins (AFB1), fumonisins (FB1) and ochratoxins (OTA) in grains were used as the detection objects, and the three-dimensional photonic crystal microsphere liquid chip was used as the carrier. A new technology for high-sensitivity, high-throughput detection of chemiluminescent liquid-phase chips built on the surface of the sphere. Background technique [0002] Mycotoxins are secondary metabolites produced by certain fungi that pollute agricultural products under certain external environments. Mycotoxins often enter the human body through the food chain and cause varying degrees of impact on the human body. Among the known mycotoxins, aflatoxins, fumonisins and ochratoxins are more harmful to human body. Based on this, countries and regions around the world have formulated strict standards to...
Claims
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IPC IPC(8): G01N33/569G01N21/76
Inventor 李建林徐坤孙越李玮徐杰蒋云坤曹斌郑铁松
Owner NANJING NORMAL UNIVERSITY
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