Preparation method for living hypertension animal model caused by high-sugar and high-fat feed feeding

A high-fat feed and animal model technology, applied in the field of medicine, can solve problems such as no animal model

Inactive Publication Date: 2013-11-13
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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Problems solved by technology

At present, the common hypertensive rat models in the laboratory include spontaneously hypertensive rats, two-kidney-one-clip hypertensive rat models, etc., but no animal models close to the pathogenesis of human hypertension have been seen. The present invention is based on human daily life In the study, the cause of human hypertension - long-term high-sugar and high-fat diet, established an animal model that can be used for the screening, pharmacological research and drug efficacy evaluation of drugs for the treatment of living hypertension

Method used

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  • Preparation method for living hypertension animal model caused by high-sugar and high-fat feed feeding
  • Preparation method for living hypertension animal model caused by high-sugar and high-fat feed feeding
  • Preparation method for living hypertension animal model caused by high-sugar and high-fat feed feeding

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Embodiment 1

[0008] 1 Experimental materials and instruments

[0009] 1.1 Animals SD rats, half male and half male, weighing 180-220 g, 20 rats. License No. SCXK (Zhejiang) 20080033, provided by Zhejiang Experimental Animal Center.

[0010] instrument ALC-NIBP non-invasive blood pressure measurement and analysis system (Shanghai Alcott Biotechnology Co., Ltd.); Holland Ritu SELECTRA-E automatic biochemical analyzer (Netherlands Ritu company); Powerwave340 microplate reader (US Bio-TEK company); DH -500K / Na / Cl / Ca / pH electrolyte analyzer (Shenzhen Baikangli Technology Co., Ltd.).

[0011] Reagent Alanine aminotransferase, aspartate aminotransferase, γ-glutamyl transpeptidase, alkaline phosphatase, total cholesterol, triglyceride, glucose, uric acid, creatinine (Shanghai Fosun Changzheng Medical Science Co., Ltd.); BUN reagent Box, Nanjing Jiancheng Institute of Bioengineering; A calibration / washing solution, B slope calibration solution, deproteinized cleaning solution, manufact...

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Abstract

The invention belongs to the technical field of medicine, and particularly relates to a preparation method for a living hypertension animal model caused by high-sugar and high-fat feed feeding. The model can be used for the screening, pharmacological studies and efficacy evaluation of medicaments for treating living hypertension. The living hypertension animal model can be established by feeding high-sugar and high-fat feeds (5 to 15 percent of sucrose, 5 to 15 percent of lard, 0.5 to 5 percent of cholesterol, 0.2 to 2 percent of bile salt and the balance of ordinary feeds) to normal SD rats in a model group every day for 8 to 17 weeks. The influence of the long-term feeding of the high-sugar and high-fat feeds on the blood pressure of the rats and functions of visceral organs such as the liver and the kidney is monitored by taking blood pressure indexes, mainly systolic pressure, as main model screening indexes and taking indexes in serum and urine as auxiliary indexes.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for preparing an animal model of active hypertension induced by high-sugar and high-fat feed feeding, and the model can be used for screening, pharmacological research and drug effect evaluation of drugs for treating active hypertension. Background technique [0002] There are many causes of high blood pressure in the human body. Among them, high blood pressure is closely related to a long-term high-sugar and high-fat diet. Regular consumption of high-sugar and high-fat foods will increase blood lipids and cholesterol, and easily form blood clots. At the same time, it is easy to cause body obesity. produce high blood pressure. The body is obese, and the increase of adipose tissue increases the body's demand for blood and increases the burden on the heart and blood vessels. The body must increase blood pressure to meet the body's blood supply needs. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A23K1/18
Inventor 吕圭源陈素红苏洁夏超群
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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