Fluorescence excitation real-time imaging system and method

Abstract

The invention discloses a fluorescence excitation real-time imaging system and method. The system comprises an optical signal acquisition module, a light source module, a system support module, a synchronous triggering module and a computer module, wherein the optical signal acquisition module is used for acquiring optical signals in an imaging area; the light source module is used for providing light source irradiation for the image area; the system support module is used for supporting equipment used in the system; the synchronous triggering module is used for synchronously triggering a CCD (Charge Coupled Device) camera and an optical filter rotating disk; and the computer module is used for performing necessary parameter setting on the system and processing and displaying image data. According to the method and the system, optical fluorescent images and white light images of an object in an imaging visual field can be acquired continuously and dynamically, and fluorescent images and white light images are fused and displayed by using the computer module, so that the problem that a single CCD camera displays continuous and dynamic fused images of the fluorescent images and the white light images is solved effectively. The system is reasonable and practical in structure, can be widely applied to the research of optical molecular image experiments and the research and development of medicines, and has a wide development prospect.

Description

technical field [0001] The invention relates to the technical field of optical molecular imaging, in particular to a real-time imaging system and method for excited fluorescence. Background technique [0002] Molecular imaging is one of the most dazzling sciences and technologies in the 21st century. It refers to the medical imaging technology that detects molecules in living organisms at the molecular level without damage and gives information about the distribution of molecules in the body. And it has been proven to be an imaging tool that can visualize the physiological and pathological changes of organisms at the molecular, gene, and cellular levels in vivo. As one of the important molecular imaging modalities, optical molecular imaging has been applied to the early detection of tumors, Drug development and other fields. [0003] Compared with traditional imaging technologies, optical molecular imaging technology has the following advantages: First, optical molecular i...

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Problems solved by technology

[0005] Excited fluorescence imaging systems currently on the market are roughly divided into two categories: one is to use a single camera for real-time imaging. When this type of product is used for real-time imaging, fluorescence real-time imaging and white light real-time imaging need to be carried out in stages, which cannot be achieved. Simultaneous fluorescence real-time imaging and white light real-time imaging, and the effect of real-time imaging is not ideal

The other is to use multiple cameras for real-time imaging. This type of product has a high imaging resolution and can simultaneously perform real-time imaging of fluorescence data and white light data. However, due to the different chip sizes of fluorescence cameras and white light cameras, and the Data and white light data are transmitted to the computer through two channels respectively, which makes it impossible for this type of product to perform real-time imaging on the fusion image of fluorescence data and white light data

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