Method for determining biodegradability of biomass pyrolysis bio-oil

Abstract

The invention discloses a method for determining the biodegradability of biomass pyrolysis bio-oil. The method comprises the steps of (1) acquiring inocula from at least 10 places where various chemical substances are used and emitted, and culturing the inocula; (2) preparing a culture medium stock solution for a test from an analytically pure reagent, and then, preparing a culture medium for a test from the stock solution; (3) determining the chemical oxygen demand (COD) of the bio-oil by using a COD determinator; (4) preparing a test liquid from the culture medium; (5) determining the oxygen volume consumed by the inocula by using a BOD (Biochemical Oxygen Demand) determinator when the bio-oil is subjected to 28-day biodegradation; and (6) dividing a COD value by a BOD value so as to calculate the fast biodegradability of the bio-oil. The method has the advantages that the operation is simple, used instruments are fewer, the data processing is easy, and the like.

Description

technical field [0001] The invention belongs to the field of rapid biodegradability determination of chemicals, and in particular relates to a method for determining the biodegradability of biomass pyrolysis bio-oil. Background technique [0002] The development of the chemical industry plays an irreplaceable role in promoting social progress. However, a large number of synthetic substances enter the natural environment and accumulate for a long time, posing a huge threat to humans and the ecological environment. They are easy to biodegrade, low-toxic or non-toxic, and environmentally friendly. Green chemicals have become a hot spot in current research and development. Biodegradability, as one of the important indicators to evaluate the environmental friendliness of organic chemicals, has been generally recognized. Establishing a scientific and effective method for evaluating the biodegradability of organic substances is of great significance for the control of organic pollu...

AI Technical Summary

Problems solved by technology

This method requires a magnetic stirrer CO 2 Measuring device, air flow meter and other instruments and equipment, the test process also needs to be based on the CO 2 The speed of manual sampling is generated, and the volume of the absorption liquid cannot be affected, and the CO is measured immediately after sampling 2 , the operation process is greatly affected by human factors, and the test data cannot be automatically and continuously recorded

However, in China, there is no report on the rapid biodegradability of bio-oil produced by pyrolysis of biomass so far. Therefore, the present invention uses oxygen consumption and As a measurement index, the oxygen consumption index of bio-oil was measured by commercial COD analyzer and BOD analyzer respectively, and a biodegradability test method especially suitable for bio-oil was proposed