Miltenberger blood group antibody detection test strip and detection method thereof

A detection method and antibody detection technology, which is applied in the field of medical detection, can solve the problems of antigen depletion, shortage of antigen reagents and antibody reagents, antibody detection restrictions, etc., and achieve the effect of ensuring clinical safety, scientific blood transfusion, and quick and easy operation

Active Publication Date: 2013-11-13
SUZHOU GUOKE MEDICAL TECH DEV CO LTD
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

Due to the lack of relevant reagents, the antibody screening cells used by most medical institutions do not contain Miltenberger blood group antigen, and routine testing cannot identify the specificity of the antibody, resulting in missed detection of patients with this antibody
Due to the short storage time of fresh red blood cells (up to 3 months), the source of Miltenberger antigen-positive red blood cells is very limited, and the detection of Miltenberger blood group screening and its antibodies is greatly limited
At present, the main problems faced in the detection of Miltenberger blood group antibodies are: 1) Because the corresponding screened red blood cells are not used in the clinical test spectrum cells, irregular antibodies are often missed; 2) The stable supply of screened red blood cells containing rare antigens cannot be obtained for a long time Guarantee; 3) The storage time of fresh red blood cells is extremely short
[0004] Due to the lack of commercially available monoclonal antibodies against Miltenberger blood group antigens, the inherent shortcomings of human-derived blood group reagents (human red blood cell reagents and human serum antibody reagents) and the lack of suitable test methods, it is currently impossible for countries around the world to carry out routine clinical trials. Miltenbergerr blood type compatibility test, only in a small number of large blood centers and blood type reference laboratories for some difficult blood type incompatibility disease patients
So far, the specific obstacles and problems that cannot routinely carry out Miltenberger blood group compatibility test in clinical practice are: the shortage of both Miltenberger antigen reagents and antibody reagents, the inherent shortcomings of red blood cell reagents and human serum antibody reagents, and the currently applied immunoserological test technology disadvantages of the method, etc.
But CSL companies use Kode TM The blood group antigen reagents prepared by the technology are still based on surviving red blood cells. The above-mentioned fundamental problems have not been solved. The blood group antigens on the membranes of surviving red blood cells are easily hemolyzed, and the antigenicity also disappears.

Method used

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  • Miltenberger blood group antibody detection test strip and detection method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] see figure 1 , a Miltenberger antibody detection immunofluorescence chromatography test strip, comprising a reaction membrane 5, a sample pad 2, an absorption pad 7 and a liner 8, the reaction membrane 5 is located on the liner 8, and the reaction membrane 5 A detection belt 4 and a quality control belt 6 are fixed, and the sample pad 2 and the absorbent pad 7 partially overlap the two sides of the reaction membrane 5 respectively, and are located on the reaction membrane 5 and the backing plate 8, The sample pad 2 includes a first sample application point 1 and a second sample application point 3, the first sample application point 1 is arranged on the side away from the sample pad 2 overlapping with the reaction membrane 5, the The second sample adding point 3 is set close to the side where the sample pad 2 overlaps with the reaction membrane 5 .

[0025] Preferably, the material of the reaction membrane 5 is one of nitrocellulose membrane, cellulose acetate membrane...

Embodiment 2

[0027] Provide a kind of detection method that the test strip described in embodiment one is used for Miltenberger blood type antibody screening:

[0028] (1) Preparation of test strips:

[0029] 1) Select Millipore HF 180 backing membrane as the reaction membrane, cut it into 30×1.8 cm size for use, adjust the concentration of human IgG and human IgM to 0.5 mg / mL with 0.01 M PBS with a pH value of 7.2, Add Tween-20 with a volume fraction of 1%, and spray it on the surface of the NC membrane as a quality control strip using a film-scribing device, and the film-scribing volume is 0.5 μL / cm. Streptavidin was adjusted to a concentration of 0.1 mg / mL using 0.15 M PBS with a pH value of 7.2, and was sprayed on the surface of the NC membrane as a detection zone using a film-drawing device, with an interval of 5 mm between each line, and the film was drawn Immediately after the end, put it in a 37°C oven to dry overnight, and store it at room temperature for later use;

[0030] 2) ...

Embodiment 3

[0048] Provide a kind of detection method that the test strip described in embodiment one is used for the specific identification of Miltenberger blood group antibody:

[0049] (1) Preparation of test strips:

[0050] 1) Select Millipore HF 180 backing membrane as the reaction membrane, cut it into 30×1.8 cm size for use, adjust the concentration of human IgG and human IgM to 0.5 mg / mL with 0.01 M PBS with a pH value of 7.2, Add Tween-20 with a volume fraction of 1%, and spray it on the surface of the NC membrane as a quality control strip 6 using a film-scribing instrument, and the film-scribing volume is 0.5 μL / cm. Streptavidin was adjusted to a concentration of 0.1 mg / mL using 0.15 M PBS with a pH value of 7.2, and was sprayed on the surface of the NC membrane as a detection zone using a film-drawing device, with an interval of 5 mm between each line, and the film was drawn Immediately after the end, put it in a 37°C oven to dry overnight, and store it at room temperature ...

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Abstract

The invention discloses a Miltenberger blood group antibody detection immunofluorescent chromatography test strip and a detection method thereof. The Miltenberger blood group antibody detection immunofluorescent chromatography test strip comprises a sample pad, a reaction film and an absorption pad which are in an ordinal overlap joint relationship. Two ends of the sample pad are provided with a first sample injection point and a second sample injection point. An inner quality control band and at least one antibody detection band are fixed to the reaction film. The detection method comprises the following steps of mixing a biotin-labelled polypeptide blood group antigen and a serum sample, adding the mixture into the sample pad, adding a fluorescent label second antibody into the sample pad, and carrying out detection by a fluorescence detector. The Miltenberger blood group antibody detection immunofluorescent chromatography test strip solves the problem that a simple and practical clinical conventional Miltenberger blood group antibody detection technology and a reagent are deficient, is used for assistant clinical diagnosis of Miltenberger blood group antibody-caused hemolytic disease of the newborn, and adverse effects of blood transfusion, and guarantees clinical safety, effectiveness and scientific blood transfusion.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a Miltenberger antibody detection immunofluorescence chromatography test strip and a detection method thereof. Background technique [0002] The MNS blood group system is the second blood group system discovered after ABO, and the number of polymorphisms in its antigens is second only to the Rh blood group system. In the MNS blood group system, there is a special Miltenberger subblood group system, whose blood group antigens are erythrocyte phenotypes combined with a series of low-frequency antigens identified by human serum immune antibodies. The occurrence frequency of several antigens such as Mur and Mil in this subsystem is less than 1 / 1000 in European Caucasians, but it is 3%-10% in Chinese and other Asian populations. A survey of 844 Dong people in Guangxi showed that the positive rate of Mur rare blood group antigen was 15.4%. In 1990, Lin Mali and others conducted a sur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80
Inventor 丁少华李勇尹焕才段生宝王红梅田晶晶陈晔洲李冬
Owner SUZHOU GUOKE MEDICAL TECH DEV CO LTD
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