Method for intermittent co-substrate-controlled co-metabolism decomposition of difficultly degraded phenol pollutants
A co-substrate and pollutant technology, applied in the field of water environmental pollution control, can solve the problems of phenolic pollutants existing, difficult to remove, and unreasonable co-substrate dosing methods, etc.
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Embodiment 1
[0021] In this embodiment, intermittent co-substrate regulation and co-metabolism decomposes refractory phenolic pollutants is achieved in this way:
[0022] 1. Adopt 250ml serum bottle to carry out experiment, the bacterial concentration of inoculation anaerobic sludge is 5g / L, the concentration of pentachlorophenol (PCP) is 9mg / L, and the total volume of water sample in the bottle is 180ml.
[0023] 2. Divide the experiments into two groups, group A and group B, each group has two parallel samples, and all serum bottles are cultured in a constant temperature shaker. The shaking speed was 120r / min, the culture temperature was 35°C, the pH was about 7.0, and the nitrogen and phosphorus concentrations in all water samples were 50mg / L and 10mg / L, respectively. The water is controlled to contain no dissolved oxygen by blowing off nitrogen, and it is in an anaerobic state.
[0024] 3. Group A experiments used pentachlorophenol alone as the substrate, group B experiments used sodi...
Embodiment 2
[0031] In this embodiment, intermittent co-substrate regulation and co-metabolism decomposes refractory phenolic pollutants is achieved in this way:
[0032] 1. A 250ml serum bottle was used for the experiment. The bacterial concentration inoculated with the aerobic sludge was 0.5g / L, the o-cresol (o-MP) concentration was 30mg / L, and the total volume of the water sample in the bottle was 180ml.
[0033] 2. Divide the experiments into two groups, group A and group B, each group has two parallel samples, and all serum bottles are cultured in a constant temperature shaker. The shaking speed was 120r / min, the culture temperature was 20°C, the pH was about 7.0, and the nitrogen and phosphorus concentrations in all water samples were 50mg / L and 10mg / L, respectively. The dissolved oxygen concentration is 0.5-5mg / L.
[0034] 3. The group A experiment used o-cresol alone as the substrate, and the group B experiment used glucose and o-cresol as the substrate, and the initial dosage con...
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