Culture method for increasing yield of fucoxanthin contained in diatom

A medium fucoxanthin and culture method technology, applied in the field of diatom culture and fucoxanthin yield culture, can solve the problems of low fucoxanthin content and unsatisfactory fucoxanthin yield

Active Publication Date: 2014-11-05
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the inventor’s previous experiments found that the content of fucoxanthin in diatoms obtained by simple heterotrophic culture of diatoms using conventional heterotrophic culture techniques is relatively low, and the content of fucoxanthin in dry powder is generally 7-8mg / g , the yield of fucoxanthin in diatoms is not ideal enough, it is necessary to further improve the existing culture technology, further increase the content and yield of fucoxanthin in diatoms, and reduce production costs

Method used

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  • Culture method for increasing yield of fucoxanthin contained in diatom

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Cultivate Cyclotella as follows:

[0048] (1) Use a 250ml Erlenmeyer flask as the culture container, with a working volume of 120ml. The activated Cyclotella was cultured heterotrophically in a shake flask, and the Cyclotella seed solution in the logarithmic growth phase was obtained after 7 days;

[0049] (2) Inoculate 10% (v / v) inoculum into newly prepared medium I for heterotrophic culture in shake flasks, and the initial biomass concentration converted to dry weight is 0.2±0.01g / L. Place the Erlenmeyer flask in a dark temperature-controlled shaker, the shaker speed is 130rpm, the temperature is controlled at 27±1°C, and the culture time is 8 days (192 hours). Samples were collected on the 6th, 7th, and 8th day of heterotrophic culture, washed by centrifugation, freeze-dried, and the content of fucoxanthin in the algae powder was tested. Among them, the formula of medium Ⅰ is: 0.66g MgSO dissolved in 1L saline 4 ·7H 2 O, 0.3g urea, 50.5mg KH 2 PO 4 , 34mg H 3 ...

Embodiment 2～5

[0055] Embodiments 2 to 5 are basically the same as in Example 1, except that the glucose concentration in the medium I used in step 2 is different, and the glucose concentrations in Examples 2 to 5 are respectively 10g / L, 15g / L, and 20g / L and 25g / L.

[0056] Embodiment 1~5 culture effect analysis:

[0057] see image 3 , when Examples 1 to 5 carried out heterotrophic culture, it was found that the biomass increased with the prolongation of the culture time. After cultivating for 8 days, the biomass dry weight concentration of Examples 1 to 5 were respectively 1.12g / L and 1.71g / L , 1.96g / L, 1.77g / L and 1.76g / L, while Example 3 (that is, the concentration of glucose in medium I was 15g / L) had the highest biomass dry weight concentration, but in the early stage (cultivation time Figure 4 ), it can be seen that there is a negative correlation between the concentration of carbon source and the content of fucoxanthin, that is, the content of fucoxanthin decreases with the increas...

Embodiment 6

[0059] Cultivate Cyclotella as follows:

[0060] (1) The activated Cyclotella shake flask was cultured heterotrophically for 7 days to obtain the seed solution in the logarithmic growth phase;

[0061] (2) The fermenter was used as the culture vessel for batch culture, and a 5L glass fermenter was selected with a working volume of 3.5L. Inject the Chlorella seed liquid into the newly prepared medium Ⅰ according to the inoculum amount of 15% (v / v), the initial biomass concentration is converted to dry weight of 0.29g / L, control the dissolved oxygen saturation > 50%, and stir The speed is 130rpm, the pH is controlled at 7.5, the culture temperature is controlled at 27±0.5°C, and the fermentation period is 9 days (216 hours). Samples were taken every 24 hours to measure the dry weight. After 3 days of culture (>72h), samples were taken every 24 hours and freeze-dried, then the pigment was extracted, and the content of fucoxanthin was quantitatively determined by liquid chromatog...

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Abstract

The invention provides a culture method for increasing the yield of fucoxanthin contained in diatom. The culture method comprises the following steps of: (1) heterotrophically culturing activated diatom for 3-8 days so that the diatom is in a logarithmic growth period; (2) inoculating a diatom culture solution obtained from the step (1) in the logarithmic growth period as a seed solution into a heterotrophic culture vessel which contains a heterotrophic culture medium (culture medium I) according to 5%-50% volume of inoculation amount to carry out heterotrophic culture for 4-12 days at the temperature of 20-34 DEG C, wherein tomato extractives are added to the heterotrophic culture medium (culture medium I) obtained from the step (2). The culture method provided by the invention can enhance the biomass concentration of the diatom and increase the fucoxanthin content of a stable diatom body, thereby increasing the yield of the fucoxanthin contained in the diatom.

Description

technical field [0001] The invention relates to a diatom cultivation method, which belongs to the field of bioengineering, in particular to a cultivation method which can effectively improve the yield of fucoxanthin in diatoms. Background technique [0002] Fucoxanthin (CAS registration number: 3351-86-8), also known as fucoxanthin, is a photosynthetic pigment widely present in brown algae such as brown algae (Phaeophyta) and diatoms (Bacillariophyceae). A type of carotenoid with strong antioxidant properties. Its molecular formula is C 42 h 58 o 6 , the chemical structural formula is: [0003] [0004] In recent years, scholars at home and abroad have found that fucoxanthin has biological activities such as scavenging free radicals, anti-cancer, regulating blood pressure, anti-obesity and anti-allergy, especially in two aspects of anti-tumor and weight loss (from the literature Mar . Drugs., 2011, 9:1806-1828). Existing studies have proved that fucoxanthin has inhi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12N13/00
Inventor 魏东俞建中
Owner SOUTH CHINA UNIV OF TECH
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