Trichoderma viridescens for preventing and treating pepper phytophthora blight and compound thereof
A technology for pepper blight and Trichoderma, applied in the direction of chemicals for biological control, methods based on microorganisms, applications, etc., to achieve strong inhibitory effect, good biocontrol potential, and strong inhibitory activity
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Embodiment 1
[0042] Isolation and identification of embodiment 1 Trichoderma strain
[0043] 1. Isolation of Trichoderma strains
[0044] Soil samples were collected from Zhangjiajie, Taibai Mountain and Mount Tai. The soil samples were collected at a depth of about 5cm under the fallen leaves or on the surface of the ground. After collection, they were put into sterile plastic bags and sealed, and brought back to the laboratory for separation. The soil samples to be separated were stored in a refrigerator at 4°C. After fully mixing the soil samples, take a small amount of soil particles and put them on the PDA containing chloramphenicol, 4 soil particles per dish, repeat 3 times. Place it in a 25°C incubator for dark culture, pick up the spores in time after Trichoderma sporulation and transfer them to a new PDA plate, cultivate in the dark at 25°C and obtain a pure culture.
[0045] 2. Identification of Trichoderma strains
[0046] 1) Morphological identification
[0047] (1) Observa...
Embodiment 2
[0064] Example 2 Screening of antagonistic Trichoderma strains
[0065] The isolated Trichoderma strain obtained in Example 1 was used as the tested strain.
[0066] (1) cellophane method
[0067] Cellophane culture method was used to detect whether Trichoderma strains could produce secondary metabolites that inhibited the growth of Phytophthora capsici. Cut the cellophane into discs with a diameter of 9 cm, separate the cellophane discs with paper sheets, wrap them after stacking, and sterilize at high temperature. Place cellophane discs in a container containing sterile water to moisten before use. Pour the PDA into a sterilized petri dish with a diameter of 9 cm. After cooling and solidifying, paste the sterilized cellophane on the surface of the culture medium under aseptic conditions, and volatilize excess water in the ultra-clean workbench.
[0068] Trichoderma strains and Phytophthora capsici strains were transferred to PDA medium for activation, and after growing fo...
Embodiment 3
[0076] Application of embodiment 3TS0404 Trichoderma strain in capsicum blight control
[0077] Preparation of biocontrol agents
[0078] (1) Preparation of Trichoderma TS0404 spore suspension
[0079] Trichoderma TS0404 was inoculated in PDA medium and cultured at 25°C for 6 days; after the cultivation was completed, the spores were scraped into sterilized water to prepare TS0404 spore suspension (the concentration of viable bacteria was 10 8 individual / mL).
[0080] (2) Preparation of Trichoderma preparation
[0081] Inoculate the Trichoderma TS0404 spore suspension into cooked and sterilized wheat grains (feed wheat grains). The amount of Trichoderma TS0404 spore suspension required per kilogram of wheat grains is 3 mL. After 18 days of alternating cultivation in the dark for 18 days, a large number of spores were produced on the medium, and the spores together with the wheat grains were Trichoderma preparations.
[0082] Biocontrol test
[0083] The biocontrol experim...
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