RT-PCR kit for detection of poultry source pedigree H3N2 subtype canine influenza virus and application thereof

A canine influenza virus, H3N2 technology, applied in the direction of DNA / RNA fragments, recombinant DNA technology, microbial-based methods, etc., can solve the problems of inability to make rapid diagnosis, virus antigenic variation, and inability to detect viruses, etc., to achieve good results The effects of coverage and versatility, prevention of influenza pandemic, and simple operation
CN103409559BActive Publication Date: 2015-05-13CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2015-05-13

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Abstract

The invention discloses an RT-PCR kit for detection of poultry source pedigree H3N2 subtype canine influenza virus and application thereof. The invention provides a primer pair for assisting detection of poultry source pedigree H3N2 subtype canine influenza virus. The nucleotide sequence of one primer is shown as a SEQ ID No.1, and the nucleotide sequence of the other primer is shown as a SEQ ID No. 2. Detection of poultry source pedigree H3N2 subtype canine influenza virus by the kit provided by the present invention is faster, and has higher singularity, sensibility and sensitivity than a traditional method. The kit can detect virus allantoic fluid as well as clinically acquired samples, and is easy for popularization as a useful tool for diagnosis and epidemiological investigation of poultry source pedigree H3N2 subtype canine influenza virus.
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Description

technical field

[0001] The invention relates to a RT-PCR reagent kit for detecting H3N2 subtype canine influenza virus of avian origin and application thereof. Background technique

[0002] Canine influenza is a highly contagious disease of dogs caused by canine influenza virus. Affected dogs often show cold symptoms such as cough and pneumonia. In recent years, the reports of canine influenza virus infection have increased rapidly. Dogs are important companion animals for human beings. People pay more and more attention to the health of dogs. In addition, influenza viruses in dogs may increase the chance of infecting humans through close human-dog contact. Therefore, canine influenza virus not only affects the health of dogs, but also poses a serious threat to public health security.

[0003] It has been reported in the literature that dogs can be infected with avian-derived H3N2 subtype canine influenza virus, equine-derived H3N8 subtype canine influenza virus, H1N1 inf...

Claims

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