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Testing box for antibacterial drug sensitivity test, and making method thereof

A technology for antibacterial drugs and sensitive testing, which is applied in biochemical equipment and methods, microbial measurement/inspection, biomass post-processing, etc. It can solve the problems of inaccurate MIC results, unfavorable large-scale detection, troubles, etc., and achieve easy observation And the effect of judgment and test operation is simple and convenient

Inactive Publication Date: 2013-12-04
NANJING YOKO PHARMA GRP CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The test tube dilution method is cumbersome to operate and is not conducive to large-scale detection; although the microdilution method uses less reagents and specimens than the test tube dilution method, it is still more troublesome and the results are not as clear as the test tube dilution method; the agar dilution method is good for mass detection, but it is not applicable In the detection of routine individual clinical specimens, and can only be tested on-site and prepared with drug-containing culture plates, it is also cumbersome
In recent years, a test strip of the concentration gradient paper method (E test) has been popular, which combines the convenience of the paper method and the dilution method to give the advantages of MIC, but the price is more expensive and the MIC results are not very accurate, gradually showing the method its own defects

Method used

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  • Testing box for antibacterial drug sensitivity test, and making method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] In this example, a small plastic box with an internal size of 6.6 cm x 0.6 cm is used, which is divided into eleven small squares. The relevant preparation and test process are as follows:

[0034] (1) Take the plastic box, plastic cover and gel strip mold prepared by molding, put them in a sterile hood and sterilize them with ultraviolet light for 30 minutes;

[0035] (2) Prepare cefotaxime to the concentration of 3.6, 7.2, 14.4, 28.8, 57.6, 115.2, 230.4, 460.8, 921.6, 1843.2μg / ml, and add them to the empty chambers of the plastic box (the second empty chamber to the eleventh 10 μl in each empty chamber, and dry under negative pressure for later use.

[0036] (3) Add 1.2% agar to the MH broth, boil in water to dissolve the agar, and autoclave at 121°C for 15 minutes;

[0037] (4) Accurately absorb 1.6ml of 1.2% hot agar solution and aseptically inject it into the strip mold. The size of the mold inside is 6.6cm×0.6cm. in a plastic bag;

[0038] (5) Inoculate variou...

Embodiment 2

[0046]In this example, a small plastic box with an inner dimension of 6.6 cm x 0.6 cm is used, which is divided into eleven empty chambers. However, the experimental bacteria adopts the fastidious bacteria Haemophilus influenzae, and the relevant preparation and test procedures are as follows:

[0047] (1) Take the test box, plastic cover and gel strip mold prepared by molding, put them in a sterile hood and sterilize them with ultraviolet light for 30 minutes;

[0048] (2) Prepare ampicillin to a concentration of 1.8, 3.6, 7.2, 14.4, 28.8, 57.6, 115.2, 230.4, 460.8, 921.6 μg / ml, and add them to the empty chambers of the plastic box (the second empty chamber to the eleventh empty chamber , the first empty chamber does not add liquid medicine), 10 μl per cell, dry under negative pressure for later use.

[0049] (3) Add 1.2% agar to HTM broth (added after coenzyme I is sterilized), boil in water to dissolve the agar, and autoclave at 121°C for 15 minutes;

[0050] (4) Accurate...

Embodiment 3

[0060] In this example, a small plastic box with an internal size of 6.6 cm x 0.6 cm is used, which is divided into eleven empty chambers. Gel strips are gelatin gels.

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Abstract

The invention provides a testing box for the antibacterial drug sensitivity test. The testing box comprises a box body (1) and a box cover (2) capable of covering the upper edge of the box body, tabulae (3) are arranged in the box body (1), chambers (4) are formed between the tabulae (3), antibacterial drugs and gel strips are contained in the chambers (4), and the stereostructure of the testing box is shown in figure 1. The invention also provides a making method of the testing box, and a method for utilizing the testing box to test the minimal inhibitory concentrations of drugs. The testing box has the advantages of stable reagent, simple method and reliable result, can be used for detecting a single specimen, and can also be used for detecting batch specimens.

Description

technical field [0001] The invention belongs to the field of in vitro diagnostic reagents, and in particular relates to a preparation method and clinical application of a bacterial (or fungal) drug susceptibility test kit. Background technique [0002] The widespread use of antibiotics and antibacterial drugs (hereinafter collectively referred to as antibacterial drugs) has led to the emergence of bacterial (or fungal) drug resistance while successfully fighting infections. Because the susceptibility of bacteria (or fungi) to antibacterial drugs will change, before choosing to use antibacterial drugs to fight infection, antimicrobial susceptibility test (AST) must be carried out in order to correctly select antibacterial drugs and Effectively control infection. [0003] Commonly used methods for susceptibility testing of antimicrobials include disk method and dilution method. Disc drug susceptibility testing is a qualitative testing method, and usually only reports the thr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12Q1/20
Inventor 童明庆
Owner NANJING YOKO PHARMA GRP CO LTD