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Method for producing bacterial cellulose by liquid spraying fermentation tower filled with non-woven fabric

A technology of liquid pouring fermentation and bacterial cellulose, which is applied in the fields of textile, food and beverage, microbes, and fermentation. It can solve the problems of abnormal flow of air and fermentation liquid, and achieve the goal of improving unit utilization, saving costs, and making up for expenses. Effect

Inactive Publication Date: 2013-12-04
ZHONGYUAN ENGINEERING COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Completely solve the problem that the film blocks the gaps between the fillers, the air and the fermentation broth cannot flow normally, and the fermentation production of bacterial cellulose is terminated.

Method used

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  • Method for producing bacterial cellulose by liquid spraying fermentation tower filled with non-woven fabric

Examples

Experimental program
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Effect test

Embodiment 1

[0031]The method for producing bacterial cellulose with the liquid pouring fermentation tower of the non-woven fabric type filler of the present embodiment comprises the following process steps:

[0032] (1) Preparation of sugar tea liquid: take raw tea leaves / sugar / water ratio 1 / 10 / 100, brew tea leaves and sugar with boiling water, keep boiling slightly for 1~10 minutes, let the tea leaves settle, take the supernatant, Sterilize at 108°C-121°C for 10-25 minutes, and set aside;

[0033] 2) Prepare the liquid bacterial liquids of Saccharomyces cerevisiae and Acetobacter xylinum respectively:

[0034] A. Preparation of Saccharomyces cerevisiae liquid: use an inoculation loop to take a ring of purified Saccharomyces cerevisiae bacteria lawn from the slant of the glucose yeast extract (GYC) solid medium to the sterilized and cooled liquid containing glucose yeast extract Place the test tube of the culture medium in an incubator at 25°C to 35°C for 3 to 7 days; obtain a liquid bac...

Embodiment 2

[0050] The method for producing bacterial cellulose with the liquid pouring fermentation tower of non-woven fabric type filler of the present invention, comprises following processing step:

[0051] (1) Preparation of sugar tea liquid: take the raw material tea leaves / sugar / water ratio 1 / 10 / 100, brew tea leaves and sugar with boiling water, keep boiling for 1 minute, let the tea leaves settle, take the supernatant, and brew at 108 Sterilize at ℃ for 25 minutes, and set aside;

[0052] 2) Prepare the liquid bacterial liquids of Saccharomyces cerevisiae and Acetobacter xylinum respectively:

[0053] A. Preparation of Saccharomyces cerevisiae liquid: use an inoculation loop to take a ring of purified Saccharomyces cerevisiae bacteria lawn from the slant of the glucose yeast extract (GYC) solid medium to the sterilized and cooled liquid containing glucose yeast extract culture medium in a test tube, placed in an incubator at 25°C for 7 days; to obtain a liquid bacterial liquid of...

Embodiment 3

[0061] The method for producing bacterial cellulose with the liquid pouring fermentation tower of non-woven fabric type filler of the present invention, comprises following processing step:

[0062] (1) Preparation of sugar tea liquid: take the raw material tea leaves / sugar / water ratio 1 / 10 / 100, brew tea leaves and sugar with boiling water, keep boiling for 10 minutes, let the tea leaves stand still, take the supernatant, and brew at 121 Sterilize at ℃ for 10 minutes, and set aside;

[0063] 2) Prepare the liquid bacterial liquids of Saccharomyces cerevisiae and Acetobacter xylinum respectively:

[0064] A. Preparation of Saccharomyces cerevisiae liquid: use an inoculation loop to take a ring of purified Saccharomyces cerevisiae bacteria lawn from the slant of the glucose yeast extract (GYC) solid medium to the sterilized and cooled liquid containing glucose yeast extract culture medium in a test tube, placed in an incubator at 35°C for 3 days; to obtain a liquid bacterial li...

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Abstract

A method for producing bacterial cellulose by a liquid spraying fermentation tower filled with non-woven fabric is disclosed. The liquid spraying fermentation tower filled with non-woven fabric is employed in a fermentation step. The liquid spraying fermentation tower filled with non-woven fabric comprises a tower body, wherein a return spray plate is disposed on the top portion in the tower body, a non-woven fabric filling system is disposed on the upper portion in the tower body, a fermentation liquid is disposed on the lower portion of the tower body, and a false bottom is disposed between the filling system and the fermentation liquid. The non-woven fabric filling system comprises a roller disposed in the tower and non-woven fabric wound on the roller, one end of the non-woven fabric is connected with an unreeling apparatus and the other end of the non-woven fabric is connected with a winding apparatus. The non-woven fabric filling system is employed and the orientation movement of the non-woven fabric is utilized, so that Haiyue produced by fermentation production of liquid sprayed kombucha, which means bacterial cellulose and bacterial cellulose membrane system aggregated thereby, is taken away. The method helps to thoroughly solve the problem that the membrane blocks the gaps in the filler and then the fermentation production of bacterial cellulose is terminated because of the failure of normal flow of the air and the fermentation liquid.

Description

technical field [0001] The invention relates to the industries and fields of microorganisms, fermentation, textiles, food and beverages, in particular to a method for producing bacterial cellulose with a liquid pouring fermentation tower with non-woven fabric filler. Background technique [0002] Cellulose is the most abundant biopolymer with the greatest economic potential on earth. Synthesizing cellulose is not a unique function of plants, some marine organisms can also synthesize animal cellulose, and some bacteria can produce extracellular cellulose more efficiently than plants in a heterotrophic manner. In order to distinguish it from other sources of cellulose, the cellulose synthesized by microorganisms is usually called "Bio-cellulose" or "Bacterial cellulose" (BC for short). Bacterial cellulose belongs to nano-scale fibers and is the thinnest among natural fibers. A typical bacterial fiber line width is only 0.1 μm, while the width of coniferous wood pulp fibers is...

Claims

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Application Information

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IPC IPC(8): C12P19/04A23F3/00C12M1/00C12R1/02C12R1/865
Inventor 张迎晨吴红艳
Owner ZHONGYUAN ENGINEERING COLLEGE
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