Artificial fermentation method capable of improving yield and quality of cordyceps sinensis

A fermentation method and technology of Cordyceps sinensis are applied in the field of artificial fermentation of yield and quality, which can solve problems such as no reports, achieve good economic benefits, improve yield and quality, and shorten the fermentation period.

Active Publication Date: 2013-12-11
青海珠峰冬虫夏草工程技术研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report in the prior art about how this factor affects the yield an

Method used

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  • Artificial fermentation method capable of improving yield and quality of cordyceps sinensis

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Embodiment 1

[0020] An artificial fermentation method capable of improving the yield and quality of Cordyceps sinensis, comprising strain cultivation on a slant, primary fermentation, secondary fermentation and solid-liquid separation process, the specific steps are:

[0021] (1) Incline culture of strains: Inoculate the strains on the slope, the culture medium contains carbon source, nitrogen source, inorganic salt and agar powder, and cultivate at 12°C for 16 days;

[0022] (2) Primary fermentation: Inoculate the strains cultured on the slant on the medium in the initial stage of primary fermentation. The medium uses glucose as the carbon source and yeast extract as the nitrogen source, wherein the glucose concentration is 20g / L, and the yeast extract The concentration is 17g / L, cultured at 14°C for 3 days; the feeding operation is carried out, the feeding medium (that is, the medium in the late stage of fermentation) also uses glucose as the carbon source and yeast extract as the nitroge...

Embodiment 2

[0026] An artificial fermentation method capable of improving the yield and quality of Cordyceps sinensis, comprising strain cultivation on a slant, primary fermentation, secondary fermentation and solid-liquid separation process, the specific steps are:

[0027] (1) Incline culture of strains: Inoculate the strains on the slope, the culture medium contains carbon source, nitrogen source, inorganic salt and agar powder, and cultivate at 12°C for 17 days;

[0028] (2) Primary fermentation: Inoculate the strains cultured on the slant on the medium in the initial stage of primary fermentation. The medium uses glucose as the carbon source and yeast extract as the nitrogen source, wherein the glucose concentration is 40g / L, and the yeast extract The concentration is 12g / L, cultured at 14°C for 4 days; the feeding operation is carried out, and the feeding medium (that is, the medium in the late stage of fermentation) also uses glucose as the carbon source and yeast extract as the nit...

Embodiment 3

[0032] An artificial fermentation method capable of improving the yield and quality of Cordyceps sinensis, comprising strain cultivation on a slant, primary fermentation, secondary fermentation and solid-liquid separation process, the specific steps are:

[0033] (1) Incline culture of strains: Inoculate the strains on the slope, the culture medium contains carbon source, nitrogen source, inorganic salt and agar powder, and cultivate at 12°C for 16 days;

[0034] (2) Primary fermentation: Inoculate the strains cultured on the slant on the culture medium at the initial stage of the primary fermentation. The medium uses glucose as the carbon source and yeast extract as the nitrogen source, wherein the glucose concentration is 32g / L, and the yeast extract The concentration is 15g / L, cultured at 14°C for 3 days; the feeding operation is carried out, the feeding medium (that is, the medium in the late stage of fermentation) also uses glucose as the carbon source and yeast extract as...

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Abstract

The invention relates to a method capable of improving yield and quality of cordyceps sinensis. The method comprises the following steps: strain slant cultivation, primary fermentation, secondary fermentation and a solid-liquid separation process, wherein each of the primary fermentation and the secondary fermentation is divided onto an initial fermentation stage and a later fermentation stage; when the initial fermentation stage is ended, the later fermentation stage is entered after the material supplementing operation; at the initial fermentation stage and the later fermentation stage, a culture medium of a carbon source and a nitrogen source which are in different concentration ratio is adopted for fermentation. According to the artificial fermentation method disclosed by the invention, the yield and the quality of the product are improved, the fermentation period is shortened, and good economic benefits are brought for artificial industrial production of cordyceps sinensis.

Description

technical field [0001] The invention relates to the technical field of an artificial fermentation method of Cordyceps sinensis, in particular to an artificial fermentation method capable of improving the yield and quality of Cordyceps sinensis. Background technique [0002] Cordyceps sinensis, also known as Cordyceps sinensis, is a traditional precious Chinese medicine in my country. It has various pharmacological effects such as relieving cough, reducing phlegm, lowering serum cholesterol, regulating and enhancing immunity, and anti-tumor. In recent years, the demand for Cordyceps sinensis has increased dramatically at home and abroad. But because Cordyceps sinensis has its strict parasitism and special growth geographical environment, the output is quite limited, and the medicine source is relatively scarce. In order to alleviate the contradiction between supply and demand in the market, artificial fermentation products of Cordyceps sinensis are becoming more and more imp...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
Inventor 王玉花王辉程振玉
Owner 青海珠峰冬虫夏草工程技术研究有限公司
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