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Tissue culture method for polyploid hemerocallis middendorfii and culture medium

A technology of Hemerocallis group and polyploid, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., and can solve the problems of unsatisfactory commercial production, simple ramet propagation speed, and restrictions on the large-scale application of polyploid Hemerocallis grandiflora, etc. problem, to achieve the effect of improving the callus differentiation rate and transplanting survival rate, accelerating the propagation speed, and stabilizing the production system

Active Publication Date: 2013-12-11
中邦生态环境有限公司 +1
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the market demand for polyploid Hemerocallis grandiflorum is increasing, and the simple reproduction speed of ramets is far from meeting the needs of commercial production, thus limiting the large-scale application of Polyploid Hemerocallis grandiflora in gardens

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of embodiment 1 MS:

[0030] The working solution concentration unit is mg / L, where:

[0031] Macroelements: NH 4 NO 3 1650, KNO 3 1900, CaCl 2 2H 2 O 440, MgSO 4 ·7H 2 O 370, KH 2 PO 4 170; trace elements: KI 0.83, H 3 BO 3 6.2. MnSO 4 4H 2 O 22.3, ZnSO 4 ·7H 2 O 8.6, Na 2 MnO 4 2H 2 O 0.25, CuSO 4 ·5H 2 O 0.0025, CoCl2 6H2O 0.0025; iron salts: FeSO4 7H2O 27.8, Na2-EDTA 2H2O 37.3; inositol 100, hydrochloric acid 0.5, pyridoxine hydrochloride 0.5, thiamine hydrochloride 0.1, glycine 2.0. Weigh the above raw materials in proportion and mix them uniformly to obtain MS.

Embodiment 2

[0032] Example 2 Induction Medium for Polyploid Hemerocallis grandiflora Tissue Culture

[0033] Weigh 1 L of MS prepared in Example 1 and add 0.2 mg / L of naphthaleneacetic acid, 0.5 mg / L of 6-benzylpurine, 0.7 mg / L of 2,4-dichlorophenoxyacetic acid, 30 g / L of sucrose, and 9 g of agar / L; pH value 5.8-6.0; Mix the above raw materials evenly.

Embodiment 3

[0034] Example 3 Subculture Proliferation Medium

[0035]Weigh 1 L of MS prepared in Example 1 and add 0.2 mg / L of naphthaleneacetic acid, 2.0 mg / L of 6-benzylpurine, 30 g / L of sucrose, and 9 g / L of agar; the pH value is 5.8-6.0; the above raw materials are mixed Get even.

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Abstract

The invention provides a tissue culture method for polyploid hemerocallis middendorfii and also discloses a culture medium applicable to the tissue culture method. The tissue culture method provided by the invention can be used for increasing the breeding coefficient of the polyploid hemerocallis middendorfii, and making up for the deficiencies of a conventional tissue culture technology, such as reducing the pollution rate of explants and increasing the callus differentiation rate and transplanting survival rate. By collecting the explants, a disinfection effect, a rooting effect and the transplanted seedling survival rate of the explants are all superior to previous tissue culture rapid propagation technologies for day lily varieties. An established large-scale commodity production system is stable, and the production cycle is only about two months, so that the breeding speed of the polyploid hemerocallis middendorfii is increased, the season limit on breeding is broken, and the industrialized production of seedling culture is realized. The tissue culture formula provided by the invention is the best combination of the mass propagation of the polyploid hemerocallis middendorfii, and can be used for quickly breeding a large number of tissue culture seedlings so as to adapt to the demand of the landscaping market.

Description

technical field [0001] The invention provides a polyploid Hemerocallis grandiflorum tissue culture method, and also discloses a culture medium suitable for the tissue culture method of the invention, belonging to the technical field of flower cultivation. Background technique [0002] Hemerocallis is a perennial flower, which has been widely used in urban greening and garden landscape construction in recent years. Among the many varieties of daylily, the polyploid daylily has rich flower colors, beautiful flowers, and green leaves. It has the advantages of easy cultivation and early germination in spring. In recent years, the market demand for polyploid Hemerocallis grandiflora has been increasing, and the simple reproduction speed of ramets is far from meeting the needs of commercial production, thus limiting the large-scale application of polyploid Hemerocallis grandiflora in gardens. Therefore, it is very important to explore the technology and method of rapid propagatio...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 温娜武术杰王丽兰高志新刘亚亮尹立辉席应琪孟庆明谭首创
Owner 中邦生态环境有限公司
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