Tissue culture method for polyploid hemerocallis middendorfii and culture medium
A technology of Hemerocallis group and polyploid, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., and can solve the problems of unsatisfactory commercial production, simple ramet propagation speed, and restrictions on the large-scale application of polyploid Hemerocallis grandiflora, etc. problem, to achieve the effect of improving the callus differentiation rate and transplanting survival rate, accelerating the propagation speed, and stabilizing the production system
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Embodiment 1
[0029] The preparation of embodiment 1 MS:
[0030] The working solution concentration unit is mg / L, where:
[0031] Macroelements: NH 4 NO 3 1650, KNO 3 1900, CaCl 2 2H 2 O 440, MgSO 4 ·7H 2 O 370, KH 2 PO 4 170; trace elements: KI 0.83, H 3 BO 3 6.2. MnSO 4 4H 2 O 22.3, ZnSO 4 ·7H 2 O 8.6, Na 2 MnO 4 2H 2 O 0.25, CuSO 4 ·5H 2 O 0.0025, CoCl2 6H2O 0.0025; iron salts: FeSO4 7H2O 27.8, Na2-EDTA 2H2O 37.3; inositol 100, hydrochloric acid 0.5, pyridoxine hydrochloride 0.5, thiamine hydrochloride 0.1, glycine 2.0. Weigh the above raw materials in proportion and mix them uniformly to obtain MS.
Embodiment 2
[0032] Example 2 Induction Medium for Polyploid Hemerocallis grandiflora Tissue Culture
[0033] Weigh 1 L of MS prepared in Example 1 and add 0.2 mg / L of naphthaleneacetic acid, 0.5 mg / L of 6-benzylpurine, 0.7 mg / L of 2,4-dichlorophenoxyacetic acid, 30 g / L of sucrose, and 9 g of agar / L; pH value 5.8-6.0; Mix the above raw materials evenly.
Embodiment 3
[0034] Example 3 Subculture Proliferation Medium
[0035]Weigh 1 L of MS prepared in Example 1 and add 0.2 mg / L of naphthaleneacetic acid, 2.0 mg / L of 6-benzylpurine, 30 g / L of sucrose, and 9 g / L of agar; the pH value is 5.8-6.0; the above raw materials are mixed Get even.
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