Unlock instant, AI-driven research and patent intelligence for your innovation.

Chitosan oligosaccharide

A technology of chitosan oligosaccharide and chitosan is applied to chitosan oligosaccharide. It can solve the problems of long degradation time, low degradation efficiency and high cost, and achieve the effects of good solubility, high yield and fast reaction speed.

Inactive Publication Date: 2014-06-25
JIANGSU SHUNXINDE BIOLOGICAL SCI & TECHCO
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For the deficiencies in the prior art, the object of the present invention is to provide a method for preparing chitosan oligosaccharides, which solves the problems of long degradation time, high cost, and low degradation efficiency in the prior art.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Dissolve 80-mesh 300KDa chitosan, D.D75%, in acetic acid or hydrochloric acid aqueous solution at a rate of 500r / min to form a mixed solution containing 3wt% chitosan and 8wt% acetic acid or containing The mixed solution of chitosan 3wt% and hydrochloric acid 8wt%;

[0035] (2) Add hydrogen peroxide to the above mixed solution so that the mixed solution of chitosan and acetic acid contains 4.5wt% hydrogen peroxide or the mixed solution of chitosan and hydrochloric acid contains 4.5% hydrogen peroxide;

[0036] (3) Place the above-mentioned solution containing chitosan, acetic acid and hydrogen peroxide or chitosan, hydrochloric acid and hydrogen peroxide in the reactor under the condition of stirring at a speed of 500r / min, ultrasonic frequency at 45KHz, and temperature at 35°C , carry out the degradation reaction for 2.5 hours;

[0037] (4) After the degradation reaction, adjust the degraded product to neutral with a strong base, filter, concentrate the filtrate, ...

Embodiment 2

[0039] (1) Dissolve 90 mesh 500KDa chitosan, D.D90%, stirring and dissolving in acetic acid or hydrochloric acid aqueous solution at a rate of 300r / min to form a mixed solution containing 7wt% chitosan and 4wt% acetic acid or chitosan The mixed liquor of sugar 7wt% and hydrochloric acid 4wt%;

[0040] (2) Add hydrogen peroxide to the above mixed solution so that the mixed solution of chitosan and acetic acid contains 6.5wt% hydrogen peroxide or the mixed solution of chitosan and hydrochloric acid contains 6.5% hydrogen peroxide;

[0041](3) Put the above-mentioned solution containing chitosan, acetic acid and hydrogen peroxide or chitosan, hydrochloric acid and hydrogen peroxide in a reactor and stir at a speed of 300r / min, the ultrasonic frequency is 65KHz, and the temperature is 55°C Under, carry out degradation reaction 4.5 hours;

[0042] (4) After the degradation reaction, adjust the degraded product to neutral with a strong base, filter, concentrate the filtrate, add et...

Embodiment 3

[0044] (1) Dissolve 100 mesh 250KDa chitosan, D.D85%, in acetic acid or hydrochloric acid aqueous solution with stirring at 250r / min to form a mixed solution containing 9wt% chitosan and 6wt% acetic acid or chitosan The mixed liquor of sugar 9wt% and hydrochloric acid 6wt%;

[0045] (2) Add hydrogen peroxide to the above mixed solution so that the mixed solution of chitosan and acetic acid contains 7.5wt% hydrogen peroxide or the mixed solution of chitosan and hydrochloric acid contains 7.5% hydrogen peroxide;

[0046] (3) Put the above-mentioned solution containing chitosan, acetic acid and hydrogen peroxide or chitosan, hydrochloric acid and hydrogen peroxide in a reactor under the conditions of ultrasonic frequency of 65KHz and temperature of 66°C under stirring at 250r / min. Degradation reaction for 5.5 hours;

[0047] (4) After the degradation reaction, adjust the degraded product to neutral with a strong base, filter, concentrate the filtrate, add ethanol for alcohol ana...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
particle sizeaaaaaaaaaa
degree of polymerizationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a chitosan oligosaccharide, which is prepared in the way that chitosan is taken as the raw material, subjected to pretreatment, feed liquid configuration, H2O2 and ultrasonic synergetic oxidative degradation, filteration and ethanol extraction and then dried. Compared with the prior art, the preparation method provided by the invention is short in degradation time and complete in degradation and can achieve high yield of 85-95%, the average molecular weight of degradation products is 1,500-3,000, the distribution is relatively narrow and the preparation method is suitable for industrialized mass production and application.

Description

【Technical field】 [0001] The invention belongs to the field of macromolecular sugars, in particular to a chitosan oligosaccharide. 【Background technique】 [0002] Chitosan (chitosan), also known as chitosan, chitosan, etc., is the only alkaline amino polysaccharide that exists in large quantities in natural sugars. It is insoluble in water and alkaline solutions, but soluble in inorganic acids such as dilute hydrochloric acid and nitric acid and Most organic acids such as acetic acid. Oligochitosan is a degradation product of chitosan, which is formed by condensing glucosamine monomers with β-(1,4)-aminoglycosidic bonds. As a biodegradable polymer material with excellent performance, it is not only soluble in Water also has unique physiological activity and physical and chemical properties, has antibacterial, inhibits the growth of tumor cells, strengthens liver function, prevents stroke, resists peptic ulcer, lowers blood sugar and blood lipids, resists radiation, prevents...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
Inventor 纪海林祁培生
Owner JIANGSU SHUNXINDE BIOLOGICAL SCI & TECHCO