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Method for carrying out colorimetric detection on glucose by using metal organic coordination compound

A metal-organic, complex technology, applied in the field of biomedical detection, can solve the problems of high cost of precious metal nanoparticles, low reactivity of metal oxides, long detection time, etc., to avoid long-term reaction, avoid high cost shortcomings, operation simple effect

Inactive Publication Date: 2013-12-11
ZHEJIANG SITAOLI SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these materials exhibit good glucose detection performance, the disadvantages are also obvious
For example, noble metal nanoparticles are expensive and difficult to promote
Metal oxides have low reactivity and long detection times

Method used

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  • Method for carrying out colorimetric detection on glucose by using metal organic coordination compound
  • Method for carrying out colorimetric detection on glucose by using metal organic coordination compound
  • Method for carrying out colorimetric detection on glucose by using metal organic coordination compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 26

[0042] A method for colorimetric detection of glucose with a metal-organic complex, comprising the steps of:

[0043] (1) Establish a working curve

[0044] Add 20 microliters of glucose oxidase aqueous solution with a concentration of 20 mg / ml into 200 microliters of glucose solutions of different concentrations (glucose solutions of different concentrations are made with 0.02M disodium hydrogen phosphate-citric acid buffer solution (pH = 7.4) Dilute the 1M glucose solution to 500 microliters with 0.02M disodium hydrogen phosphate-citric acid buffer solution with a pH value of 7.4, incubate at 37°C for 30 minutes, add 20 microliters at a concentration of 50 mmol / One liter of 3,3', 5,5'-tetramethylbenzidine in dimethyl sulfoxide solution and 20 microliters of the metal-organic complex solution prepared in Example 17, with 0.2M disodium hydrogen phosphate at pH=3.4- Dilute the citric acid buffer solution to 1 ml, record the relationship between different glucose concentration...

Embodiment 27

[0049] A method for colorimetric detection of glucose with a metal-organic complex, comprising the steps of:

[0050]Add 10 microliters of glucose oxidase aqueous solution with a concentration of 20 mg / ml to 200 microliters of the glucose sample to be tested, and dilute to 500 microliters with 0.02M disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution with a pH value of 6 , incubate at 37°C for 15 minutes, add 2 microliters of 50 mmol / L 3,3',5,5'-tetramethylbenzidine in dimethyl sulfoxide solution and 10 microliters prepared in Example 16 The metal-organic complex solution was diluted to 1 ml with 0.2M citric acid-sodium hydroxide-hydrochloric acid buffer solution with a pH value of 3 to obtain solution A, and the absorbance value of solution A in the visible light region was measured, and glucose was detected colorimetrically. concentration.

Embodiment 28

[0052] A method for colorimetric detection of glucose with a metal-organic complex, comprising the steps of:

[0053] Add 20 microliters of glucose oxidase aqueous solution with a concentration of 20 mg / ml to 200 microliters of the glucose sample to be tested, and dilute to 500 microliters with 0.02M disodium hydrogen phosphate-potassium dihydrogen phosphate buffer solution with a pH value of 7 , incubate at 37°C for 40 minutes, add 100 μl of 2,2'-azino-bis-(3-ethylbenzodihydrothiazoline-6-sulfonic acid) diammonium at a concentration of 50 mmol / l The dimethyl sulfoxide solution of the salt and 100 microliters of the metal-organic complex solution prepared in Example 18 were diluted to 1 milliliter with 0.2M citric acid-sodium citrate buffer solution with a pH value of 2 to obtain solution A, and to measure the solution A is the absorbance photometric value in the visible light region, and the concentration of glucose is detected colorimetrically.

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Abstract

The present invention discloses a method for carrying out colorimetric detection on glucose by using a metal organic coordination compound. The method comprises: (1) preparing an inorganic salt solution of a variable valence metal, wherein the inorganic salt solution is a solution I; (2) preparing a 2-methylimidazole solution adopted as a solution II; (3) mixing the solution I and the solution II to prepare a metal organic coordination compound solution; and (4) adding a glucose oxidase aqueous solution to a glucose sample requiring detection, diluting with a buffer solution, culturing at a temperature of 37 DEG C, adding a dimethyl sulfoxide solution of a peroxidase color source substrate and the metal organic coordination compound solution, diluting with a buffer solution to obtain a solution A, determining an absorbance value of the solution A in the visible light region, and carrying out colorimetric detection to obtain a glucose concentration. With the method, a detection time can be substantially shortened, operations are simple, cost is low, the defect of high cost due to use of metal nanoparticles is avoided, and the defect that the reaction requires a long time during use of the metal oxide and other mimic enzymes is avoided.

Description

technical field [0001] The invention belongs to the field of biomedical detection and relates to a method for colorimetric detection of glucose with a metal organic complex. Background technique [0002] Glucose is an important biomolecule, and its content in the blood is an indicator of the health of the human body. Therefore, rapid and accurate detection of glucose content is of great significance for monitoring human health (distinguishing between healthy and sub-healthy), and making early warning and diagnosis of certain diseases, such as diabetes [0003] The detection method of glucose is mainly electrochemical method. For example, Chinese invention patent (200910067282) reported a non-enzymatic electrochemical glucose sensor based on nickel nanoparticles / carbon fiber composite material, and Chinese invention patent (200810124404.6) disclosed a tubular metal based sensor. Materials for nonenzymatic electrochemical biosensors. Electrochemical sensors have high detecti...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/78
Inventor 翟俊峰刘作家李子意韩绪儒李聪聪
Owner ZHEJIANG SITAOLI SCI & TECH CO LTD
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