A method for quantitative determination of nanoparticles using Raman spectroscopy

A technology of Raman spectroscopy and nanoparticles, applied in the field of quantitative determination of nanoparticles by Raman spectroscopy, to achieve the effect of simple method, rich measurement methods and high sensitivity

Active Publication Date: 2015-09-23
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, with the continuous in-depth research of quantum dots, the principle of fluorescence enhancement or quenching based on quantum dots has been frequently reported in the detection of heavy metal cations, small organic molecules and drug molecules.

Method used

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  • A method for quantitative determination of nanoparticles using Raman spectroscopy
  • A method for quantitative determination of nanoparticles using Raman spectroscopy
  • A method for quantitative determination of nanoparticles using Raman spectroscopy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Put 0.1 g of L-cysteine ​​(L-Cysteine, L-Cys) granular solid in a round bottom flask, add 100 mL of deionized water, stir to dissolve at room temperature, add 0.25 mmol of AgNO 3 , and adjust the pH=11 with NaOH, then add 0.10mmolNa 2 S, finally obtained brownish-red Ag 2 S colloidal quantum dots were stored in the refrigerator for later use.

[0018] (2) Take the solution obtained in step (1) and dilute it with double distilled water to obtain a concentration of 0.3090×10 3 ppbAg 2 S colloidal quantum dot solution; 19.9×10 in 500uL 3 ppb silver colloid, 100uL concentration of 1mol / L NaCl solution and 100uL concentration of 5.23×10 -5 mol / L Rh6G solution as the base, take 12 volumetric flasks, use a pipette gun to accurately measure the base solution into the volumetric tubes, and then add the concentration of 0.3090×10 3 ppbAg 2 S colloidal quantum dot solution 0 uL, 5.6 uL, 10.6 uL, 15.6 uL, 20.6 uL, 25.6 uL, 30.6 uL, 35.6 uL, 50.6 uL, 60.6 uL, 70.6 uL and 8...

Embodiment 2

[0022] (1) Ag is prepared in Example 1 2 S colloidal quantum dots, stored at room temperature until use.

[0023] (2) Take the solution obtained in step (1) and dilute it with double distilled water to obtain a concentration of 0.3090×10 3 ppbAg 2 S colloidal quantum dot solution; 19.9×10 in 500uL 3 ppb silver colloid, 100uL concentration of 1mol / L NaCl solution and 100uL concentration of 5.23×10 -5 mol / L Rh6G solution as the base, take 10 volumetric flasks, use a pipette gun to accurately measure the base solution into the volumetric tubes, and then add the concentration of 0.3090×10 3 ppbAg 2 S colloidal quantum dot solution 0uL, 1uL, 2uL, 3uL, 8uL, 13uL, 18uL, 23uL, 28uL, 33uL, dilute to 2mL, so as to make Ag with a concentration of 0-5.10ppb 2 S colloidal quantum dot solution system.

[0024] (3) Same as step (3) of Example 1.

[0025] (4) With different concentrations of Ag in the solution system 2 The relative peak height of the Raman spectrum of S colloidal quan...

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Abstract

The invention discloses a method for quantitatively measuring nanoparticles by using Raman spectrum. First, prepare the Ag2S colloidal solution in the water phase, and store it under normal temperature and freezing conditions until use; secondly, dilute the Ag2S colloidal solution to an appropriate concentration and add it to the substrate containing silver colloid, NaCl, and rhodamine 6G respectively, and make different formulations. concentration of the solution; finally, the prepared solution was taken as the blank sample without the addition of Ag2S sample, and the DXR smart Raman spectrometer was used to sample, collect the sample peak, and observe the change of the sample characteristic peak at 1508cm-1 with different concentrations. Calculate the peak intensity corresponding to the collected sample peak intensity and the blank sample, and then use the relative peak intensity to plot the concentration. The method of the invention is simple and sensitive, and enriches the determination methods of nanoparticles.

Description

technical field [0001] The invention relates to a method for measuring the content of nanoparticles by using Raman spectrum. Background technique [0002] Inorganic semiconductor nanocrystals, often referred to as colloidal quantum dots. Quantum dots have attracted extensive interest of researchers at home and abroad because of their unique physical and chemical properties different from macroscopic materials. At present, with the continuous in-depth research of quantum dots, the principle of fluorescence enhancement or quenching based on quantum dots has been frequently reported in the detection of heavy metal cations, small organic molecules and drug molecules. Different from the method of measuring the concentration of cations by using the fluorescence quenching of quantum dots by metal cations, the present invention is based on the mechanism of fluorescence enhancement or quenching of active surfaces based on surface-enhanced Raman scattering (SERS): fluorescent substan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
Inventor 覃爱苗吴秀兰蒋丽廖雷余心亮
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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