Preparation method of porous dressing of silk fibroin-calcium alginate biological wound

A calcium alginate and wound surface technology, which is applied in the fields of skin care products and medical devices, can solve problems such as poor skin healing effect, secondary physical damage to the skin, and poor slow-release effect of drugs, and achieve excellent air permeability and water absorption. The effect of excellent sex and accelerated wound healing

Active Publication Date: 2013-12-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the poor hydrophilic performance of traditional wound dressings, the wound dressings are hard and brittle, and often cause secondary physical damage to the injured skin, and bacteria can enter the edge of the dressing to grow; second...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Dissolve the fibrous silk fibroin formed after silkworm cocoon degumming in a LiBr solution, and obtain a 3% (w / w) silk fibroin solution after filtration, dialysis, and concentration;

[0021] (2) Mix 80-120mg of calcium alginate and 8-12mL of silk fibroin solution, heat, dissolve and stir in a water bath at 40-60°C, so that the calcium alginate and silk fibroin are cross-linked to obtain a gel-like solution;

[0022] (3) Pour the gel-like solution obtained in (2) into a molding vessel to solidify the gel to obtain a dressing film;

[0023] (4) The dressing film obtained in (3) is frozen at -20°C to -60°C and then dried by a freeze dryer to obtain a wound gel dressing. Example 2

Embodiment 2

[0024] (1) Dissolve the fibrous silk fibroin formed after silkworm cocoon degumming in CaCl concentration 2 In the solution, a 5% (w / w) silk fibroin solution was obtained after filtration, dialysis and concentration;

[0025] (2) Mix 80-120mg of calcium alginate and 8-12mL of silk fibroin solution, heat, dissolve and stir in a water bath at 40-60°C, so that the calcium alginate and silk fibroin are cross-linked to obtain a gel-like solution;

[0026] (3) Pour the gel-like solution obtained in (2) into a molding vessel to solidify the gel to obtain a dressing film;

[0027] (4) The dressing film obtained in (3) is frozen at -20°C to -60°C and then dried by a freeze dryer to obtain a wound gel dressing;

[0028] (5) Immerse the wound dressing in (4) in 75% (v / v) ethanol solution for 12 hours to fix the formed gel, then replace it with deionized water to remove the residual ethanol, and obtain the wound gel after natural drying dressing.

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PUM

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Abstract

The invention relates to a preparation method of porous dressing of a silk fibroin-calcium alginate biological wound in the technical field of skin care products and medical apparatuses and instruments. The preparation method specifically comprises the steps: dissolving fibrous silk fibroin formed after a silkworm cocoon is degummed, filtering, dialyzing and concentrating so as to obtain silk fibroin water solutions with different weight concentrations; mixing deionized water and calcium alginate with a defined amount of silk fibroin solution, placing the obtained gelatinous solution in an environment with a normal temperature to obtain a gel film, freezing and drying the gel film to obtain the porous dressing of the fibroin-calcium alginate biological wound. The excellent biocompatibility is realized; the deionized water and the calcium alginate in the raw materials are both natural active materials, are used commonly used for the health-care food industry without stimulation on the skin, and are good in hygroscopicity and water-absorbing quality; the silk fibroin and the calcium alginate both have the high water holding rate property, thereby being relatively good in affinity property on the skin.

Description

technical field [0001] The invention relates to the technical field of skin care products and medical devices, in particular to a preparation method of silk fibroin-calcium alginate biological wound dressing. Background technique [0002] The skin is the largest organ of the human body and the first physical barrier between the human body and the external environment. It plays an important role in preventing the invasion of harmful substances from the outside, excreting and preventing water evaporation, regulating body temperature, and keeping the body in a stable internal environment. The use of different wound dressings can effectively promote the repair of damaged skin and accelerate wound healing. Due to the poor hydrophilic performance of traditional wound dressings, the wound dressings are hard and brittle, and often cause secondary physical damage to the injured skin, and bacteria can enter the edge of the dressing to grow; secondly, the traditional wound dressing has...

Claims

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Application Information

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IPC IPC(8): A61L15/28A61L15/32A61L15/44
Inventor 杨明英帅亚俊周官山朱良均
Owner ZHEJIANG UNIV
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