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Kitchen waste decomposition bacterial agent and preparation method thereof

A technology for decomposing food waste and decomposing bacteria, which is applied in the field of microorganisms and can solve the problems of high operating costs

Active Publication Date: 2013-12-25
THE QUARTERMASTER EQUIPMENT RESEARCH INSTITUTE OF THE GENERAL LOGISITIC DEPARTME
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing technology mostly adopts a high-temperature process of 60-80 °C, and the equipment operation cost is high

Method used

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  • Kitchen waste decomposition bacterial agent and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1, preparation of food waste decomposing bacteria agent

[0059] 1. Preparation of single bacterial powder of each strain

[0060] The strains of Bacillus subtilis, Saccharomyces cerevisiae, Aspergillus oryzae, Trichoderma viride, and Aspergillus niger were cultured in high density respectively, and then expanded culture, and finally the cultured single bacteria were prepared into dry bacteria powder.

[0061] 1. Preparation of Bacillus subtilis single bacterial powder

[0062] (1) Activation culture of Bacillus subtilis

[0063] Bacillus subtilis activation medium: glucose 18g / L, peptone 10g / L, sodium chloride 3g / L and beef extract 0.4g / L, the balance is water; pH is natural pH.

[0064] Inoculate the strains of Bacillus subtilis (Bacillus subtilis) CICC10066 on the slant culture of the purified culture to the shake flask filled with the activation medium with a volume ratio of 16% (if the volume of the shake flask is 100mL, then the amount of activation m...

Embodiment 2

[0131] Embodiment 2, preparation of food waste decomposing bacteria agent

[0132] 1. Preparation of single bacterial powder of each strain

[0133] The strains of Bacillus subtilis, Saccharomyces cerevisiae, Aspergillus oryzae, Trichoderma viride, and Aspergillus niger were cultured in high density respectively, and then expanded culture, and finally the cultured single bacteria were prepared into dry bacteria powder.

[0134] 1. Preparation of Bacillus subtilis single bacterial powder

[0135] (1) Bacillus subtilis activation medium: glucose 16g / L, peptone 12g / L, sodium chloride 4g / L, beef extract 0.3g / L, the balance is water; pH is natural pH.

[0136] Inoculate the purified Bacillus subtilis CICC10066 strain on the slant culture into a shake flask filled with a 20% volume ratio of activated medium. Activation culture in the bottle for 26h (OD600 of the bacterial suspension is 2.05).

[0137] (2) Expanded culture of Bacillus subtilis and acquisition of bacterial powder ...

Embodiment 3

[0185] Embodiment 3, preparation of food waste decomposing bacteria agent

[0186] 1. Preparation of single bacterial powder of each strain

[0187] The strains of Bacillus subtilis, Saccharomyces cerevisiae, Aspergillus oryzae, Trichoderma viride, and Aspergillus niger were cultured in high density respectively, and then expanded culture, and finally the cultured single bacteria were prepared into dry bacteria powder.

[0188] 1. Preparation of Bacillus subtilis single bacterial powder

[0189] (1) Activation culture of Bacillus subtilis

[0190] Bacillus subtilis activation medium: glucose 18g / L, peptone 12g / L, sodium chloride 3g / L and beef extract 0.5g / L, the balance is water; pH is natural pH.

[0191] Inoculate the purified Bacillus subtilis CICC10066 strain on the slant culture into the shake flask filled with the activation medium with a volume ratio of 25%. Activation culture in the bottle for 28h (OD600 of the bacterial suspension is 2.06).

[0192] (2) Expanded c...

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Abstract

The invention discloses a kitchen waste decomposition bacterial agent and a preparation method and application thereof. The kitchen waste decomposition bacterial agent is prepared by mixing the following substances in parts by weight: 30-50 parts of bacillus subtilis, 10-30 parts of saccharomyces cerevisiae, 10-30 parts of aspergillus oryzae, 10-30 parts of trichoderma viride and 10-30 parts of aspergillus niger. In the invention, the strains of bacillus subtilis, saccharomyces cerevisiae, aspergillus oryzae, trichoderma viride and aspergillus niger are subjected to high-density culture and amplification culture, and finally, each single cultured bacterium is prepared into dry bacterial powder; the bacterial powder is compounded by reasonable proportioning according to the purpose to obtain the kitchen waste decomposition bacterial agent for treating the kitchen waste. The kitchen waste decomposition bacterial agent disclosed by the invention has the advantages that (1) single bacteria can be reasonably proportioned for the kitchen waste according to different components so that the single bacteria cooperate with each other to degrade the kitchen waste; (2) the effective viable count of the single component of the compound bacterial agent is high, a small amount of bacterial agent is used for treating and degrading the kitchen waste, the cost is low, the bacterial agent is convenient to use, and the decomposition effect is good.

Description

technical field [0001] The invention belongs to the field of microorganisms, relates to a food waste decomposing bacterial agent, and also relates to a preparation method and application of the food waste decomposing bacterial agent. Background technique [0002] With the rapid development of social economy and the acceleration of urbanization process, the generation of urban domestic waste in my country continues to increase. Kitchen waste accounts for about 37% to 62% of domestic waste. Compared with ordinary waste, kitchen waste has the following characteristics: 1. High oil content and high viscosity; 2. High salt content, which makes it difficult for microorganisms to survive; 3. High stacking density Not easy to ventilate; 4. High moisture content; 5. High organic matter content. Improper disposal of food waste will rot and deteriorate, emit foul smell, spread bacteria and viruses, breed mosquitoes and flies, and pollute the environment. But from another point of vie...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C12N1/14B09B3/00C12R1/125C12R1/865C12R1/69C12R1/885C12R1/685
Inventor 绳以健何永水刘玉德王新全胡欣
Owner THE QUARTERMASTER EQUIPMENT RESEARCH INSTITUTE OF THE GENERAL LOGISITIC DEPARTME
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