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Acer palmatum Seiryu tissue culture propagation process

A technology of Acer palmatum and Qinglong, which is applied in the field of plant tissue culture, can solve the problems that Qinglong tissue culture propagation methods have not been reported, and achieve the effects of low production cost, short reproduction cycle and simple formula

Active Publication Date: 2014-01-01
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is a relatively common and precious ornamental variety in the market today, but there has never been a report on the tissue culture propagation method of Qinglong

Method used

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  • Acer palmatum Seiryu tissue culture propagation process
  • Acer palmatum Seiryu tissue culture propagation process
  • Acer palmatum Seiryu tissue culture propagation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The tissue culture propagation process of Acer chinensis in chicken feet, it includes the following steps:

[0023] S1. Collect disease-free stems of the same year at noon or afternoon on a sunny day, remove excess leaves, and cut them into stems with buds. Sterilize the stems with alcohol for 30s, then sterilize with mercury chloride for 5 minutes, and then Wash 4-6 times with sterile water;

[0024] S2, inoculate the stem segment with buds after sterilization on a medium for growth, the medium is MS+NAA 0.3mg / L, and the inoculated culture material is placed in a light intensity of 2000~3000LX, and the light time is 16h / d, the temperature is 24 ~ 26 ℃ culture room, culture for 15 days, inducing the germination of axillary buds, such as figure 1 shown;

[0025] S3, subculture proliferation culture, cut off the germinated axillary buds, inoculate on the subculture medium, the subculture medium is MS+6-BA 1.5mg / L +NAA 0.3mg / L, and the inoculated culture The material is...

Embodiment 2

[0028] The tissue culture propagation process of Acer chinensis in chicken feet, it includes the following steps:

[0029] S1. Collect disease-free stems of the same year at noon or afternoon on a sunny day, remove excess leaves, and cut them into stems with buds. The stems with buds are first sterilized with alcohol for 50s, then sterilized with mercury chloride for 4min, and then Wash 4-6 times with sterile water;

[0030] S2, inoculate the stem segment with buds after sterilization on a medium for growth, the medium is MS+NAA 0.3mg / L, and the inoculated culture material is placed in a light intensity of 2000~3000LX, and the light time is 16h / d, the temperature is 24 ~ 26 ℃ culture room, culture for 2 weeks, inducing the germination of axillary buds, such as figure 1 shown;

[0031] S3, subculture proliferation culture, cut off the germinated axillary buds, inoculate on the subculture medium, the subculture medium is MS+6-BA 1.5mg / L +NAA 0.3mg / L, and the inoculated culture...

Embodiment 3

[0034] The tissue culture propagation process of Acer chinensis in chicken feet, it includes the following steps:

[0035] S1. Collect disease-free stems of the same year at noon or afternoon on a sunny day, remove excess leaves, and cut them into stems with buds. The stems with buds are first sterilized with alcohol for 60s, then sterilized with mercuric chloride for 3 minutes, and then Wash 4-6 times with sterile water;

[0036] S2, inoculate the stem segment with buds after sterilization on a medium for growth, the medium is MS+NAA 0.3mg / L, and the inoculated culture material is placed in a light intensity of 2000~3000LX, and the light time is 16h / d, the temperature is 24 ~ 26 ℃ culture room, culture for 3 weeks, inducing the germination of axillary buds, such as figure 1 shown;

[0037] S3, subculture proliferation culture, cut off the germinated axillary buds, inoculate on the subculture medium, the subculture medium is MS+6-BA 1.5mg / L +NAA 0.3mg / L, and the inoculated c...

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Abstract

The invention discloses an acer palmatum Seiryu tissue culture propagation process which comprises the following steps: S1, collecting the current-year shoots of disease-free acer palmatum Seiryu, removing redundant leaves, shearing cutting into stem sections with buds, and sterilizing; S2, inoculating each sterilized stem section with the bud to a culture medium for growth, and inducing the germination of axillary buds; S3, carrying out subculture for multiplication, namely cutting off the germinating axillary buds, inoculating the cut germinated axillary buds on a subculture medium, and inducing the generation of multiple shoots; S4,carrying out rooting culture, cutting adventitious buds of the multiple shoots, inoculating on a rooting culture medium, and inducing the generation of a root. The acer palmatum Seiryu tissue culture propagation process disclosed by the invention can be used for constructing a Seiryu tissue culture propagation system, and realizing the breakthrough of the Seiryu propagation system, and is low in production cost, high in propagation coefficient, and short in propagation period, and is an effective way for the fast propagation of acer palmatum Seiryu and capable of preventing the virus accumulation, and by using the process, the tissue culture seedlings with consistent inheritable characters can be obtained.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to the tissue culture propagation technique of Acer palmatum Qinglong. Background technique [0002] The genus Acer (Aceraceae) is usually referred to as maple, and it is also called "maple" or "maple" in habit. There are more than 200 kinds of maple trees in the world, distributed in Asia, Europe, North America and northern Africa. There are more than 160 species in my country, accounting for more than 75% of the world's total. They are distributed all over the country, and are mainly produced in the Yangtze River Basin and the provinces to the south. Maple trees can be used as building materials, paper-making raw materials, medicine extraction, industrial oil and nectar plants. Some maple trees are rich in tree shape, elegant in posture, rich in leaf shape and changeable in leaf color, and are known as world-famous foliage plants. Acer palmatum (Acer palmatum), als...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 马建华王小辉朱晓菲吴佳川沈香兰
Owner SICHUAN COLORLINK CO LTD
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