Biological agent for compost treatment of livestock manure

A technology of biological inoculum and livestock and poultry manure, applied in the field of biological inoculum, can solve the problems of resource waste and environmental pollution, and achieve the effects of fast detonation speed, less nutrient loss of stacking materials, and long duration of high temperature

Inactive Publication Date: 2014-01-01
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the disposal methods of urban fallen leaves, rural straw and livestock and poultry manure in my country adopt unscientific

Method used

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  • Biological agent for compost treatment of livestock manure
  • Biological agent for compost treatment of livestock manure
  • Biological agent for compost treatment of livestock manure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of embodiment 1 thermophilic streptomyces carbon monoxide bacterial agent

[0025] Inoculate Streptomyces carbonmonoxide thermophilic Streptomyces CF1 on the slant of Gaoshi No. 1 solid medium, and cultivate it at a constant temperature of 50°C for 72 hours to obtain slant seeds;

[0026] The slant seeds were inoculated into Gaoshi No. 1 liquid medium, and cultured at a constant temperature of 50°C and at a speed of 180r / min for 72 hours to obtain liquid seeds;

[0027] Inoculate the liquid seed into the production medium in the liquid fermenter with 5% (V / V, that is, inoculate 5ml liquid seed per 100ml liquid medium) inoculum amount, and carry out strain production. Under continuous aeration, 50°C Cultured at constant temperature for 4 days. The cultured fermented liquid can be used as bacterial agent.

[0028] The composition of the Gaoshi No. 1 solid medium is: add 20.0 g of soluble starch per 1000 ml of distilled water, KNO 3 1.0g, K 2 HPO 4 0.5...

Embodiment 2

[0030] Embodiment 2 preparation method of Bacillus brevis bacterial agent

[0031] Bacillus brevis CX1 and Bacillus licheniformis (CICC No.20085, purchased from China Industrial Microbiology Culture Collection Management Center) were respectively inoculated on the slant of beef extract peptone solid medium, and cultured at a constant temperature of 50°C for 72 hours to obtain Bacillus brevis and slanted seeds of Bacillus licheniformis;

[0032] The slant seeds were inoculated into the beef extract peptone liquid medium, and cultured at a constant temperature of 50°C and at a speed of 180r / min for 72 hours to obtain liquid seeds;

[0033] Inoculate the liquid seed into the production medium in the liquid fermenter with 5% (V / V, that is, inoculate 5ml liquid seed per 100ml liquid medium) inoculum amount, and carry out strain production. Under continuous aeration, 50°C Cultured at constant temperature for 4 days. The cultured fermented liquid can be used as bacterial agent.

[0...

Embodiment 3

[0036] Embodiment 3 polonium penicillium inoculant preparation method

[0037] Penicillium polonium (Wang Liang, 2012) was inoculated on the slant of the improved Gaoshi No. 1 solid medium, and cultured at a constant temperature of 35°C for 72 hours to obtain slant seeds;

[0038] The slant seeds were inoculated into the improved Gaoshi No. 1 liquid medium, and cultured at a constant temperature of 35°C and at a speed of 180r / min for 72 hours to obtain liquid seeds;

[0039] Inoculate the liquid seeds into the production medium in the liquid fermenter with 5% (V / V, that is, inoculate 5ml of liquid seeds per 100ml of liquid medium) inoculation amount, and carry out the production of strains, under continuous aeration, at 35°C Cultured at constant temperature for 4 days. The cultured fermented liquid can be used as bacterial agent.

[0040] The composition of the improved Gaoshi No. 1 solid medium is: glucose 10.0g / L, peptone 5.0g / L, KNO 3 1.0g / L, K 2 HPO 4 0.5g / L, MgSO 4 ...

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Abstract

The invention discloses a biological agent for compost treatment of livestock manure. The biological agent contains Streptomyces thermocarboxydus, Brevibacillus brevis, Penicillium polonicum and Bacillus licheniformis. When the biological agent is inoculated into a pile body, the temperature of the livestock manure can rise quickly in cold autumn and winter and be kept for a relatively long time, so that the livestock manure can be quickly decomposed and converted into a high-quality organic fertilizer. The biological agent can also be used for mixed compost treatment of the livestock manure and urban and rural agricultural and forestry wastes under the condition of a relatively low ambient temperature.

Description

technical field [0001] The invention belongs to the field of resource utilization, and in particular relates to a biological bacterial agent used for composting livestock and poultry manure. Background technique [0002] For the serious pollution caused by animal husbandry, foreign developed countries have recognized it earlier, and have strict regulations on the amount of livestock and poultry manure and sewage discharge in the breeding industry. On the basis of the relevant laws of the People's Republic of China, the "Law on the Proper Management and Promotion of the Use of Livestock Excrement" was enacted. The US "Federal Water Pollution Law" focuses on the construction and management of livestock and poultry farms and stipulates that livestock manure and sewage are not allowed to be discharged into self-provided septic tanks without treatment; German laws stipulate that livestock manure and urine cannot be discharged without treatment, and stipulate that the unit The li...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C05F17/00C12R1/465C12R1/80C12R1/10C12R1/01
CPCY02W30/40
Inventor 刘克锋王顺利王红利高程达石爱平
Owner BEIJING UNIV OF AGRI
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