HPLC (high performance liquid chromatography) method for determining ten synthetic colorants in cosmetic

Abstract

The invention discloses an HPLC method for determining ten synthetic colorants in a cosmetic simultaneously. According to the method, tetrahydrofuran, dimethyl sulfoxide and methyl alcohol are used for extracting the colorants in a sample under the auxiliary condition of ultrasonic wave, the colorants are separated on a chromatographic column of EclipseXDB-C18 (3.5 mu m, 4.6*150mm) through high-speed centrifugal clarification, acetonitrile and 0.02 mol / L of an ammonium acetate solution with the pH of 4.6 are taken as mobile phases for gradient elution, a diode array detector is used for performing determination, and quantification is performed with an external standard method. The method can simultaneously determine ten synthetic colorants of pigment red 57, pigment orange 5, acid yellow 36, pigment red 53, acid purple 49, rhodamine B, solvent blue 35, Sudan red II, Sudan red IV and disperse yellow 3, and the determination minimum is 20 mg / Kg. The method is simple, fast, sensitive and suitable for quantitative determination of colorants in oily, powdery and paste cosmetics.

Description

technical field [0001] The invention relates to the technical field of chromatographic analysis of cosmetics, in particular to pigment red 57 (C.I.15850), pigment orange 5 (C.I.12075), acid yellow 36 (C.I.13065), pigment red 53 (C.I.15585), acid violet 49 (C.I.42640), Rhodamine B (C.I.45170), Solvent Blue 35 (C.I.61554), Sudan Red II (C.I.12140), Sudan Red IV (C.I.26105) and Disperse Yellow 3 (C.I.11855) and other 10 synthetic colorants high performance liquid chromatography method. Background technique [0002] Cosmetics contain a variety of chemical substances. Organic synthetic pigments are widely used in beauty cosmetics such as nail polish, eye shadow, and lipstick because of their strong covering and coloring power. However, many synthetic colorants are harmful to the human body, and some can cause skin allergies, and even cause cancer, teratogenicity, and mutagenesis. [0003] The 10 organic synthetic colorants involved in the present invention belong to azo, anthra...

AI Technical Summary

Problems solved by technology

Spectrophotometry has low sensitivity and is susceptible to interference; micellar fluorescence method has high sensitivity, but it is only suitable for colorants that can produce fluorescence, such as rhodamine B; high performance liquid chromatography-tandem mass spectrometry has high sensitivity and is applicable It is suitable for the detection of most colorants, but it is expensive; high performance liquid chromatography is widely used in the analysis and determination of harmful substances in cosmetics due to its high sensitivity and fast analysis speed.

A method capable of simultaneously detecting 10 prohibited and restricted synthetic colorants in cosmetics, such as Pigment Red 57, has not been reported yet

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