Yellow mushroom standardized component manufacturing method and application of components to treatment of liver cancer

A technology of yellow mushroom and preparation method, which is applied in the directions of biochemical equipment and method, drug combination, and microorganism determination/inspection, can solve the problem of little research on the chemical composition of the fruiting body of Armillaria yellow-green fungus, etc., and achieves expansion of raw material sources, The effect of expanded use and easy implementation

Inactive Publication Date: 2014-01-08
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The researches on Armillaria chrysogenum in the above literatures mainly focus on biotransformat

Method used

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  • Yellow mushroom standardized component manufacturing method and application of components to treatment of liver cancer
  • Yellow mushroom standardized component manufacturing method and application of components to treatment of liver cancer
  • Yellow mushroom standardized component manufacturing method and application of components to treatment of liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 The preparation method of standardized components of chanterelles comprises the following steps:

[0035] ⑴After drying and crushing the chanterelles, extract 3 times with analytical pure water at 40°C according to the solid-liquid ratio of 1g:4mL, and centrifuge to collect the fungus residue, which is mixed with acetone at a ratio of 1g:1mL Extract at 10°C for 3 times, each time for 72 hours, and collect the filtrate by centrifugation; the filtrate is concentrated under reduced pressure to a paste under the conditions of a vacuum of 0.03 MPa and a temperature of 30°C to obtain the acetone extract.

[0036] (2) Add 1 times its volume of n-hexane-ethyl acetate mixture to the acetone extract to dissolve, and pass through a 0.45 μm organic filter to obtain a sample solution containing acetone extract; 10μm silica gel is used as the separation matrix to prepare chromatographic separation to obtain 14 standardized component samples of Fr1, Fr2, Fr3, Fr4, Fr5, F...

Embodiment 2

[0047] Example 2 The preparation method of standardized components of chanterelles comprises the following steps:

[0048] ⑴After drying and crushing the chanterelle mushrooms, extract them with analytically pure water at 90°C for 3 times at a solid-liquid ratio of 1g:20 mL, each time for 1 hour, and collect the fungus residue by centrifugation. The ratio of solid to liquid was extracted at 80°C for 3 times, each time for 5 hours, and the filtrate was collected by centrifugation; the filtrate was concentrated under reduced pressure to a paste under the conditions of a vacuum of 0.1 MPa and a temperature of 80°C to obtain the acetone extract.

[0049] (2) Add 6 times its volume of n-hexane-ethyl acetate mixture to the acetone extract to dissolve, and pass through a 0.45 μm organic filter to obtain a sample solution containing acetone extract; 10μm silica gel is used as the separation matrix to prepare chromatographic separation to obtain 14 standardized component samples ...

Embodiment 3

[0053] Example 3 The preparation method of standardized components of chanterelles comprises the following steps:

[0054] ⑴After drying and crushing the yellow mushrooms, extract them with analytical pure water at 65°C for 3 times according to the solid-liquid ratio of 1g:12 mL, each time for 5.5 hours, and collect the fungus residue by centrifugation. The ratio of solid to liquid was extracted 3 times at 45°C for 38 hours each time, and the filtrate was collected by centrifugation; the filtrate was concentrated under reduced pressure to a paste under the conditions of a vacuum of 0.06 MPa and a temperature of 55°C to obtain the acetone extract.

[0055] (2) Add 3.5 times its volume of n-hexane-ethyl acetate mixture to the acetone extract to dissolve, pass through a 0.45 μm organic filter to obtain a sample solution containing acetone extract; 10μm silica gel is used as the separation matrix to prepare chromatographic separation to obtain 14 standardized component sampl...

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Abstract

The invention relates to a yellow mushroom standardized component manufacturing method. The method comprises the following steps: (1) drying and crushing yellow mushrooms, then performing water extraction, centrifugally collecting mushroom residues, extracting the mushroom residues with acetone, centrifugally collecting filtrate, and performing reduced pressure concentration on the filtrate to form paste, namely an acetone extract; (2) dissolving the acetone extract in an n-hexane-ethyl acetate mixed solution, then filtering to obtain a sample solution containing the acetone extract, and separating the sample solution containing the acetone extract on a preparation chromatogram to obtain 14 standardized component samples, namely Fr1, Fr2,..., Fr13 and Fr14; and (3) performing anti-liver cancer cell model screening in vitro on the 14 standardized component samples by an iCelligence real-time label-free cell analyzer, thus determining that the 7th, the 8th, the 9th, the 10th, the 11th, the 13th and the 14th standardized component have human liver cancer cell adherence and proliferation inhibition activity in vitro. The yellow mushroom standardized component manufacturing method provided by the invention has the advantages of simple process and easiness in implementation. Simultaneously, the obtained active components can be applied to treatment of liver cancer.

Description

technical field [0001] The invention relates to the technical fields of medicinal chemistry and biomedicine, in particular to a preparation method of standardized components of yellow mushrooms and its application in liver cancer treatment. Background technique [0002] Primary liver cancer is one of the common malignant tumors in my country. According to the latest statistics, about 600,000 new liver cancer patients are diagnosed every year in the world, ranking fifth among malignant tumors. The incidence of liver cancer in my country accounts for more than half of the world, and the age of onset tends to be younger. At present, the annual death rate of liver cancer in my country is about 20 / 100,000, ranking second in the death rate of various malignant tumors in my country, and has been on the rise in the past 10 years. Liver cancer has become a major killer that seriously threatens the health and life of our people, and its danger cannot be ignored. [0003] Studies ha...

Claims

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Application Information

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IPC IPC(8): A61K36/06A61P35/00C12Q1/02
Inventor 王启兰党军陶燕铎梅丽娟史强强顾朋嫒马琳李园园
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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