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Microbiological solid-state seed producing and fermenting device

A fermentation device and microorganism technology, applied in the field of microbial reaction devices, can solve the problems of difficulty in production, low degree of automation, low production efficiency, etc., and achieve the effects of reducing the infection rate of miscellaneous bacteria, high degree of automation, and shortening production time

Active Publication Date: 2014-01-08
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Today, microbial seed production technology has a large number of spore powders, which leads to production difficulties and seriously restricts the industrialization and industrial application of microbial engineering technology.
Most of the solid-state fermentation equipment in the prior art focuses on a certain process of microbial fermentation, such as the combination of air filtration and fermentation, fermentation and inoculation, with single functions and cannot integrate multi-functional and intensive technologies, resulting in Long production time, low production efficiency and high rate of bacterial infection
[0004] CN 101974409B disclosed a bacterial culture fermentation tank device and its fermentation process on May 1, 2013. The top of the fermentation tank is provided with cleaning and disinfection injection pipes, feeding pipes and air input pipes. The fermentation tank has a PLC controller, and its input The end is connected with the temperature sensor, pH value sensor, dissolved oxygen value sensor and stirring speed sensor arranged in the tank body. The lower side of the fermentation tank body is provided with a discharge pipe, a cleaning and disinfection pipe, a pure steam input pipe, an injection water input pipe, The acid-base washing solution input pipe, the purified water input pipe and the domestic drinking water input pipe are connected, and the pure steam input pipe is bypassed to the air intake pipe provided with an air filter. However, this invention is a liquid fermentation device and cannot achieve solid fermentation. Fermentation, the function is relatively simple
[0005] CN 102443535B disclosed a solid-state fermentation bioreactor on May 1, 2013. The reactor includes a reaction box, and a porous material layer partition is arranged in the middle of the reaction box, and the reaction box is divided into upper fermentation The reactor also includes a fermentation control system, which is used to control the stirring unit, temperature control unit, humidity control unit, air supply control unit and fermentation tail gas detection and processing unit to ensure temperature, humidity, Ventilation, stirring and tail gas real-time monitoring and control, this invention can only carry out the fermentation process, and needs to be inoculated, dried and crushed in other equipment, resulting in long production time
[0006] Both of the above two technical solutions have the problem of manual feeding and retrieving, low degree of automation, high infection rate of bacteria, and the inlet and outlet are set on the top or bottom of the fermentation tank, making it difficult to observe the operation situation

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0033] refer to Figure 1~4 , using solid strains for microbial solid-state seed production and fermentation, specifically including the following steps:

[0034] 1) Steaming and sterilizing: automatically add solid culture into the feed port 67 of the fermenter 6 through the feeding device

[0035]Base, after opening the vacuum pump 8 to keep the vacuum state in the fermenter 6, close the vacuum pump 8, and feed steam into the jacket 11 to carry out high-temperature sterilization;

[0036] 2) Inoculation: Into the fermenter 6, pass through the sterile compressed air obtained by the fine air filter 3 for sterilization, cool the solid culture to the temperature required for inoculation, insert the solid bacteria, start the stirring device, and fermenter 6 The internal solid medium is fully mixed and then fermented;

[0037] 3) Strain culture: set the temperature, humidity, ventilation and stirring speed required for the culture of the strain, open

[0038] Heater 12 enters t...

Embodiment 2

[0046] refer to Figure 1~4 , using liquid strains for microbial solid-state seed production and fermentation, the difference from Example 1 is that in step 2), when the solid medium in the fermenter 6 reaches a temperature that can be inoculated, the liquid strains are inserted, and the The liquid strain is sprayed onto the solid culture medium through the nozzle from the feeding liquid inlet 62, the stirring device is turned on, and the strain culture is carried out after being fully mixed with the solid culture medium in the fermenter 6.

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Abstract

The invention provides a microbiological solid-state seed producing and fermenting device, comprising a fermenting system, an air supply system, a temperature control system, a solid material system, a liquid-state material system and an electric control system, wherein the fermenting system comprises a fermenting tank provided with a stirring device, the top part of the fermenting tank is provided with a material liquid inlet, an exhaust port, a pressure gauge interface and an air inlet sequentially, a feed port is arranged on the upper right side of the front end of the fermenting tank, a discharge port is arranged on the lower left side of the front end of the fermenting tank, and the middle parts of both the feed port and the discharge port are provided with observation windows; the solid material system comprises a feeding device and a conveyer belt; the electric control system mainly comprises a stirring control unit, a temperature control unit, a material liquid control unit, an air supply control unit, a feeding and discharging control unit and a display. The device provided by the invention integrates the functions of steam sterilizing, fermenting, inoculating, drying and crushing of a solid culture medium, the automation degree is high, the microbial contamination rate is low, the production time is greatly shortened, the production efficiency is improved, and the stability of quality of a product is guaranteed.

Description

technical field [0001] The invention relates to a microbial reaction device, in particular to a microbial solid-state seed production and fermentation device. Background technique [0002] Microbial fermentation technology is a technology that uses certain characteristics of microorganisms to carry out industrial-scale production through modern engineering technology. It mainly includes the selection of industrial production bacteria, the control and optimization of fermentation conditions, the design of fermentation tanks and the separation of products. , extraction and refining processes. The general fermentation process divides the fermentation methods into two categories: solid-state fermentation and liquid-state fermentation according to the different physical properties of the medium. However, the active ingredients of the products obtained from liquid-state fermentation are less than those of solid-state fermentation, and solid-state fermentation technology has been d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/36C12M1/04C12M1/02
CPCC12M21/16C12M27/06C12M29/04C12M29/14C12M41/00C12M41/22C12M41/26C12M41/42
Inventor 向洋吴建华
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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