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Pretreatment method assists in realizing wheat straw high-efficiency enzymatic hydrolysis conversion

A technology of enzymatic hydrolysis and pretreatment, applied in the direction of fermentation, etc., can solve problems such as patent publications that have not been found, and achieve the effects of saving economic costs, saving energy, and improving high-efficiency conversion

Active Publication Date: 2014-01-08
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Through the search, no patent publications related to the patent application of the present invention have been found

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  • Pretreatment method assists in realizing wheat straw high-efficiency enzymatic hydrolysis conversion

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Embodiment 1

[0032] A pretreatment method capable of realizing efficient enzymatic hydrolysis conversion of wheat straw, the steps are as follows:

[0033] ⑴ First, cut and screen the raw materials of wheat straw, select about 5cm of wheat straw, and wash with water for 3-4 times;

[0034] (2) Soak the selected wheat straw in hot water at 95°C for 20 minutes, and then place the wheat straw soaked in hot water in a screw extruder for extrusion. The compression ratio of the screw extruder is 4 :1;

[0035] (3) Put the wheat straw processed by screw extrusion into the cooking tank, add 1.5% (w / w) (relative to the mass of dry wheat straw) H 2 SO 4(analytically pure, mass concentration is 98%), then add distilled water, the ratio of the mass of dry wheat straw to the total liquid volume after adding distilled water is 1:10, and cook at a temperature of 150°C. The heating time during the cooking process is controlled At 60 minutes, the holding time is 50 minutes, after a certain degree of dil...

Embodiment 2

[0045] A pretreatment method capable of realizing efficient enzymatic hydrolysis conversion of wheat straw, the steps are as follows:

[0046] ⑴ First, cut and screen the raw materials of wheat straw, select about 5cm of wheat straw, and wash with water for 3-4 times;

[0047] (2) Soak the selected wheat straw in hot water at 95°C for 20 minutes, and then place the wheat straw soaked in hot water in a screw extruder for extrusion. The compression ratio of the screw extruder is 4 :1;

[0048] (3) Put the wheat straw processed by screw extrusion into the cooking tank, add 10% (relative to the mass of dry wheat straw) NaOH, and then add distilled water, the ratio of the mass of dry wheat straw to the total liquid volume after adding distilled water is 1 : 10, carry out cooking, cooking temperature is 150 ℃, the heating time in the cooking process is controlled at 60 minutes, and the holding time is 50 minutes, through a certain degree of dilute sulfuric acid or alkali pretreatme...

Embodiment 3

[0058] A pretreatment method capable of realizing efficient enzymatic hydrolysis conversion of wheat straw, the steps are as follows:

[0059] ⑴ First, cut and screen the raw materials of wheat straw, select about 5cm of wheat straw, and wash with water for 3-4 times;

[0060] (2) Soak the selected wheat straw in hot water at 95°C for 20 minutes, and then place the wheat straw soaked in hot water in a screw extruder for extrusion. The compression ratio of the screw extruder is 4 :1;

[0061] (3) Put the wheat straw processed by screw extrusion into the cooking tank, add 10% (relative to the mass of dry wheat straw) NaOH, and then add distilled water, the ratio of the mass of dry wheat straw to the total liquid volume after adding distilled water is 1 :10, carry out cooking, cooking temperature is 150 ℃, the heating time in the cooking process is controlled at 60 minutes, and the holding time is 50 minutes, through a certain degree of alkali pretreatment to extract part of the...

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Abstract

The invention relates to a pretreatment method assists in realizing wheat straw high-efficiency enzymatic hydrolysis conversion. The method comprises the steps that: wheat straws are soaked and subjected to screw extrusion; H2SO4 or sodium hydroxide is added; distilled water is added; steaming is carried out, screw extruder extrusion is carried out, and constant-temperature water bath is carried out; two-stage grinding treatment, screening, and enzymatic hydrolysis are carried out; under a sterile condition, enzyme solution is added, and constant-temperature culturing and enzymatic hydrolysis are carried out; and the wheat straw hydrolysate is obtained after enzymatic hydrolysis. According to the invention, parts of existing devices in a pulping industry are sufficiently utilized, and the pretreatment process can be completed without additional investment, such that great economic cost is saved. Also, with the method, existing factories can be effectively fused with biomass refining industry, such that composite biomass refining industry can be developed, and sustainable development of pulping and papermaking industries is benefited.

Description

technical field [0001] The invention belongs to the technical field of biomass utilization, in particular to a pretreatment method capable of realizing efficient enzymatic hydrolysis conversion of wheat straw. Background technique [0002] With the rapid development of human society, coal, oil, and natural gas resources in nature are decreasing day by day, and the problems of global warming, environmental pollution and other environmental protection problems caused by their use have become increasingly prominent. For human society, it is imminent to find and use green, clean and renewable energy. As a result, the concept of "biomass refining (Biorefinery)" has become one of the important strategic research directions of countries all over the world. It is predicted that by 2020, 50% of organic chemicals and materials will be produced from biomass raw materials, and the period from 2000 to 2020 will be a critical period for all countries in the world to vigorously develop bi...

Claims

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Application Information

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IPC IPC(8): C12P19/14
Inventor 刘苇侯庆喜
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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