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Fermentation production method of safe efficient lactobacillus product

A production method and technology of lactic acid bacteria are applied in the field of bioengineering to achieve the effects of high product efficiency, strong quality controllability and reduction of feeding costs

Active Publication Date: 2014-01-08
广州康代生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mixed fermentation can obtain some unique products, but pure fermentation is difficult to achieve

Method used

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  • Fermentation production method of safe efficient lactobacillus product

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Effect test

Embodiment 1

[0025] Embodiment 1 Fermentative production method of safe and efficient lactic acid bacteria product of the present invention

[0026] The fermentative production method of a kind of safe high-efficiency lactic acid bacteria product of the present invention specifically comprises the following steps:

[0027] 1. Scrape Lactobacillus casei, Lactobacillus plantarum and Pichia powder from the slant of the strains, and use drinking water to make a bacterial suspension, measure the weight of the strains in each bacterial suspension, and then press the cheese milk Bacillus, Lactobacillus plantarum and powdered Pichia pastoris are mixed in a weight ratio of 1:1:1 to prepare a bacterial suspension to obtain the original strain of mixed fermentation;

[0028] 2. Inoculate the mixed fermentation strains in the primary culture medium with an inoculum amount of 10%-15% for shaking table fermentation, control the fermentation temperature at 35-37°C, and obtain the first-class seed liquid...

Embodiment

[0031] Example 2 Fermentation production method of safe and efficient lactic acid bacteria products according to the present invention

[0032] The fermentative production method of a kind of safe high-efficiency lactic acid bacteria product of the present invention specifically comprises the following steps:

[0033] 1. Scrape Lactobacillus casei, Lactobacillus plantarum and Pichia powder from the slope of the strains, and use drinking water to make a bacterial suspension, measure the weight of the strains in each bacterial suspension, and then press the cheese milk Bacillus, Lactobacillus plantarum and powdered Pichia pastoris are mixed in a weight ratio of 2:1:1 to prepare a bacterial suspension to obtain the original strain of mixed fermentation;

[0034] 2. Inoculate the mixed fermentation strains in the primary expansion culture medium with an inoculation amount of 10%-15%, control the fermentation temperature at 35-37°C, and obtain the primary fermentation liquid afte...

Embodiment 3- Embodiment 4

[0036] Embodiment 3-embodiment 4 The fermentative production method of safe high-efficiency lactic acid bacteria product of the present invention

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Abstract

The invention relates to a method for producing a safe high-biomass lactobacillus product by plant-derived multi-microbe mixed fermentation, belonging to the field of bioengineering. The fermentation strain in the fermentation production method of the lactobacillus product is an associate strain composed of Lactobacillus casei, Lactobacillus plantarum and powdery Pichia pastoris. The associate strain is sequentially subjected to primary amplification culture and secondary amplification culture, and used in large-scale production of the lactobacillus product at the inoculum size of 10-15%. The method reasonably utilizes the synbiosis among the microbes, the fermentation strain used in fermentation has higher controllability, and the end product has higher biomass; and therefore, the obtained lactobacillus product is safer and more efficient when being used in animal culture, and thus, is suitable for wide application in agricultural production.

Description

Technical field [0001] The present invention is a biological engineering field, which involves a fermentation method of a biological product, which specifically involves a fermented production method for safe and efficient lactic acid bacteria products. Background technique [0002] The role of microorganisms in agriculture has gradually been recognized by people.The microorganisms used in agriculture can be divided into fermented liquid and frozen dried powder according to the form of product expression. It is used for animal and aquaculture, which can improve the environment of animal intestinal tract, promote digestion and absorption, improve immune capacity, promote rapid growth, reduce drug use, Avoid the effects of veterinary drug residues; for agricultural planting, its advantages are low cost, good application effects, do not pollute the environment, not only increase production after use, but also improve or improve the quality of agricultural products and reduce the amo...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P7/56C12R1/245C12R1/25C12R1/84
Inventor 陈明仁
Owner 广州康代生物科技有限公司
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