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Production method of powder containing 2-o-α-d-glucosyl-l-ascorbic acid anhydrous crystals

An ascorbic acid and glucose-based technology is applied in the field of manufacturing powder containing 2-O-α-D-glucosyl-L-ascorbic acid anhydrous crystals, and can solve the problems of poor universality of the manufacturing method and the like

Active Publication Date: 2016-08-17
HAYASHIBARA BIOCHEMICAL LAB INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the above-mentioned production method, in order to increase the production rate of ascorbic acid 2-glucoside in the reaction solution obtained by the enzyme reaction to 35% by mass or more, it is necessary to separate the limited specific CGT enzyme alone or with isoamylase and other starch debranching enzymes are used in combination, so it is not suitable as a production method with poor versatility

Method used

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  • Production method of powder containing 2-o-α-d-glucosyl-l-ascorbic acid anhydrous crystals
  • Production method of powder containing 2-o-α-d-glucosyl-l-ascorbic acid anhydrous crystals

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Experimental program
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Effect test

Embodiment approach

[0091] 1. Definition of terms

[0092] In this specification, the following terms have the following meanings.

[0093]

[0094] The "degree of crystallinity of ascorbic acid 2-glucoside anhydrous crystals" as used in this specification means a numerical value defined by the following formula [1].

[0095] Formula 1]:

[0096] 【Number 1】

[0097]

[0098] h 100 : Analytical value of the degree of crystallinity obtained from powder X-ray diffraction characteristics of a powder standard sample containing ascorbic acid 2-glucoside anhydrous crystals substantially composed of ascorbic acid 2-glucoside anhydrous crystals

[0099] h 0 : Analytical value of the degree of crystallinity obtained from powder X-ray diffraction characteristics of a powder standard sample containing ascorbic acid 2-glucoside, which is basically composed of an amorphous part

[0100] h s : The analytical value of the degree of crystallinity obtained from the powder X-ray diffraction characteristi...

Embodiment 1

[0415]

[0416] 4 mass parts of liquefied potato starches were heated and dissolved by adding 20 mass parts of water, and 3 mass parts of L-ascorbic acid were added to adjust the pH to 5.5 to obtain a substrate solution. To this, 100 units of CGT derived from Geobacillus stearothermophilus Tc-91 strain (Accession No. FERMBP-11273 of the Patent Organism Deposit Center of the National Institute of Advanced Industrial Science and Technology) was added per 1 g of solid content of liquefied potato starch Enzyme crude enzyme solution (manufactured by Hayashibara Biochemical Research Institute Co., Ltd.), react at 55°C for 40 hours, together with ascorbic acid 2-glucoside, generate 2-O-α-maltosyl-L-ascorbic acid, 2-O-α α-glycosyl-L-ascorbic acid such as maltotriosyl-L-ascorbic acid and 2-O-α-maltotetraosyl-L-ascorbic acid.

[0417] After heating the reaction solution to inactivate the enzyme, adjust the pH to 4.5, add 50 units of glucoamylase agent (marketed by Amano Enzyme Co., Lt...

Embodiment 2

[0423]

[0424] When making CGTase act on a solution containing liquefied starch and L-ascorbic acid, pullulanase (marketed by Hayashibara Biochemical Research Institute Co., Ltd., product code (EN201), Pneumonia K Klebsiella pneumoniae (Aerobacter aerogenes) source, and when crystallizing ascorbic acid 2-glucoside anhydrous crystallization, it is suitable to cool the temperature of the solution from 40°C to 35°C over 1.5 days Thereafter, except for the quasi-controlled cooling method of cooling from 35° C. to 15° C. in 0.5 days, a powder containing ascorbic acid 2-glucoside anhydrous crystals was produced in the same manner as in Example 1. In addition, the reaction liquid after glucoamylase treatment The production rate of ascorbic acid 2-glucoside in is about 29.5%.The ascorbic acid 2-glucoside content of the solution used for the crystallization of ascorbic acid 2-glucoside anhydrous crystals is 91.8% in anhydrous conversion.

[0425] This product has a purity of 99.5% o...

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Abstract

[Problem] The problem is to provide a method for producing a powder containing ascorbic acid 2-glucoside anhydrous crystals, which can produce a powder that is remarkably difficult to consolidate even when the production rate of ascorbic acid 2-glucoside is less than 35% by mass. [Solution] By providing a solution comprising allowing CGTase to act on any substance containing liquefied starch or dextrin and L-ascorbic acid, and then acting glucoamylase on it, the production rate of ascorbic acid 2-glucoside is 27% by mass or more the step of the solution; the solution obtained is purified to make the ascorbic acid 2-glucoside content exceed 86% by mass; the step of separating out the anhydrous crystal of ascorbic acid 2-glucoside by the controlled cooling method or the quasi-controlled cooling method; collecting and separating out The anhydrous crystal of ascorbic acid 2-glucoside is solved by the manufacturing method of the powder containing the anhydrous crystal of ascorbic acid 2-glucoside in mature and drying steps.

Description

【Technical field】 [0001] The present invention relates to a method for producing a powder containing 2-O-α-D-glucosyl-L-ascorbic acid anhydrous crystals, and more specifically, to a powder containing 2-O-α-D - A method for producing a powder of glucosyl-L-ascorbic acid anhydrous crystals. 【Background technique】 [0002] L-ascorbic acid has been used in various applications including foods, cosmetics, etc. due to its excellent physiological activity and antioxidative effect. On the other hand, L-ascorbic acid is unstable due to direct reduction, is easily oxidized and decomposed, and has major disadvantages of easily losing physiological activity. In order to solve this disadvantage of L-ascorbic acid, the present applicant, in Patent Document 1, as one of the co-applicants, disclosed 2-O-α in which one molecule of D-glucose is bonded to the hydroxyl group at the 2-position of L-ascorbic acid. -D-glucosyl-L-ascorbic acid (Hereafter, in this specification, this is abbreviate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/04A61K31/7048A23L33/15
CPCC07H17/04A61K31/7048C07H1/06C12P19/18C12P19/60C07B2200/13C12Y204/01019A61K9/16A61P39/06A61P43/00C12P19/44C12P17/04
Inventor 涩谷孝伊泽精祐西本友之福田惠温三宅俊雄
Owner HAYASHIBARA BIOCHEMICAL LAB INC
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