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Preparation method for avian influenza virus split vaccine

An avian influenza virus and avian influenza technology, applied in the field of preparation of avian influenza virus split vaccine, can solve the problems of multi-epitope mucosal immunization vaccines without good curative effect, and achieve the effect of great application value, strong immunogenicity and low cost

Inactive Publication Date: 2014-01-22
CHENGDU KANGHUA BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In terms of vaccine research against avian influenza H7N9 virus, there is no mucosal immune vaccine with good efficacy against H7N9 subtype in the prior art, and there is no multi-epitope mucosal immune vaccine with good efficacy against H7N9 subtype virus. The epidemic of possible avian influenza H7N9 virus infection, there is an urgent need for a new and highly effective vaccine to prevent avian influenza

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation method of the avian influenza virus split vaccine of the present embodiment comprises the following steps:

[0023] The H7N9 strain avian influenza virus from NIBSC was inoculated into 11-day-old SPF chicken embryos to establish the main generation seed batch virus of avian influenza, and the virus species with high hemagglutination titer were selected from the main generation seed batch virus of avian influenza as Work seed batches to establish poisonous seeds;

[0024] The above-mentioned obtained H7N9 avian influenza working seed batch virus species is diluted to 10 with sterilized physiological saline. -5 Dilution, inoculated into the allantoic cavity of 10-day-old healthy SPF chicken embryos, the virus inoculation volume was 0.1ml / embryo, and the chicken embryos were cultured at 33°C for 40 hours; live embryos were selected and kept at 4°C for 15 hours, and the virus allantois was harvested liquid;

[0025] Formaldehyde was added to the obtained v...

Embodiment 2

[0030] The preparation method of the avian influenza virus split vaccine of the present embodiment comprises the following steps:

[0031] Inoculate 11-day-old SPF chicken embryos with the H7N9 strain of avian influenza virus from NIBSC to establish a batch of avian influenza main generation seed batch viruses, and select viruses with high hemagglutination titers from the avian influenza main generation seed batches of viruses as Work seed batches to establish poisonous seeds;

[0032] The above-mentioned obtained H7N9 avian influenza working seed batch virus species is diluted to 10 with sterilized physiological saline. -5 Dilution, inoculated into the allantoic cavity of 10-day-old healthy SPF chicken embryos, the virus inoculation volume was 0.1ml / embryo, and the chicken embryos were cultured at 35°C for 60 hours; live embryos were selected and placed in cold embryos at 4°C for 20 hours, and the virus allantois was harvested liquid;

[0033] Add formaldehyde to the obtain...

Embodiment 3

[0038] The preparation method of the avian influenza virus split vaccine of the present embodiment comprises the following steps:

[0039] The H7N9 strain avian influenza virus from NIBSC was inoculated into 11-day-old SPF chicken embryos to establish the main generation seed batch virus of avian influenza, and the virus species with high hemagglutination titer were selected from the main generation seed batch virus of avian influenza as Work seed batches to establish poisonous seeds;

[0040] The above-mentioned obtained H7N9 avian influenza working seed batch virus species is diluted to 10 with sterilized physiological saline. -5 Dilution, inoculated into the allantoic cavity of 10-day-old healthy SPF chicken embryos, the virus inoculation volume was 0.1ml / embryo, and the chicken embryos were cultured at 34°C for 50 hours; live embryos were selected and placed in cold embryos at 4°C for 17 hours, and the virus allantois was harvested liquid;

[0041] Formaldehyde was added...

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Abstract

The invention discloses a preparation method for an avian influenza virus split vaccine. The preparation method comprises the following steps of constructing H7N9 avian influenza working seed lot virus seeds, inoculating and cultivating viruses, inactivating the viruses, clarifying and concentrating virus harvest liquid, purifying the viruses, splitting the viruses, inactivating the viruses again, removing sugar and a splitting agent, filtering bacteria, performing filtration and preparing semi-finished products. The H7N9 influenza vaccine prepared by the method is high in immunogenicity, wide in antigen spectrum, stable in quality, high in purity, high in safety, low in cost and suitable for large-scale production and has a large application value.

Description

technical field [0001] The invention belongs to the field of vaccine preparation, and in particular relates to a preparation method of an avian influenza virus split vaccine. Background technique [0002] Avian influenza is the abbreviation of avian influenza. It is an infectious disease caused by type A influenza virus. It is designated as a type A infectious disease by the International Office of Epizootics. The main symptoms of the disease are high fever, cough, runny nose, myalgia, etc. Most of them are accompanied by severe pneumonia. In severe cases, heart, kidney and other organs fail and cause death. The case fatality rate is very high. At present, vaccines are mainly used for prevention. The vaccines used include virus inactivated vaccines, attenuated vaccines, recombinant subunit vaccines and the like. [0003] The outer membrane of influenza virus particles is covered by two types of surface glycoproteins, one type is hemagglutinin (ie H), the other type is neura...

Claims

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Application Information

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IPC IPC(8): A61K39/145A61P31/16
Inventor 侯文礼冯晓
Owner CHENGDU KANGHUA BIOLOGICAL PROD
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