A kind of malic enzyme gene sgme1 and its application

Abstract

The invention discloses a gene encoding malic enzyme SgME1 and its application. the malic enzyme gene SgME1 The nucleotide sequence such as SEQ? ID? As shown in NO:1, the amino acid sequence of the encoded protein is shown as SEQ? ID? NO:2 shown. SgME1 Genes regulating stylomalate synthesis, overexpressed SgME1 Promotes malic acid synthesis and increases aluminum tolerance in yeast, bean hairy root and Arabidopsis thaliana.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to malic enzyme gene SgME1 and its application. Background technique [0002] Aluminum toxicity is one of the main obstacles to crop growth on acidic soils (Kochian et al., 2004). When the pH is less than 5, the aluminum in the soil exists in an active form, which can be quickly absorbed by the plant roots (Kinraide, 1991), and has a toxic effect on the growth of the root tips, eventually leading to a decrease in crop yield. [0003] Aluminum ions can interact with cell walls and symplasts, thereby disturbing the normal physiological and biochemical reactions of plant cells (Kochian et al., 2005). Studies have shown that aluminum is easily bound to the negatively charged cell wall pectin, thereby reducing the extensibility of the cell wall and limiting cell elongation (Yang et al., 2011). In addition to combining with the cell wall, aluminum can also accumulate in the symplast,...

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Problems solved by technology

[0006] Through the early screening of aluminum tolerance, we found that the phosphorus-inefficient Stylo genotype Fine-stem is sensitive to aluminum stress, and the phosphorus-efficient Stylo genotype TPRC2001-1 has good tolerance to aluminum toxicity (Due et al., 2009), and The aluminum tolerance of TPRC2001-1 is equivalent to that of rice, but there are few studies on the mechanism of aluminum tolerance of stylophyllum

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