Speed or explosive power relative gene-ACTN3 (Alpha-Actinin 3) polymorphism rapid detection kit and detection method

A technology of polymorphism detection and explosive force, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, etc., can solve the problems of cumbersome operation, poor repeatability, and low accuracy, and achieve low detection cost, high accuracy, Ease of application and promotion

Inactive Publication Date: 2014-01-22
GUANGZHOU INST OF SPORTS SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have different defects, such as cumbersome operation, poor repeatability, and low accuracy.

Method used

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  • Speed or explosive power relative gene-ACTN3 (Alpha-Actinin 3) polymorphism rapid detection kit and detection method
  • Speed or explosive power relative gene-ACTN3 (Alpha-Actinin 3) polymorphism rapid detection kit and detection method
  • Speed or explosive power relative gene-ACTN3 (Alpha-Actinin 3) polymorphism rapid detection kit and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Amplification reagents consist of components in the following volumes:

[0059]

[0060]

Embodiment 2

[0062] Amplification reagents consist of components in the following volumes:

[0063]

Embodiment 3

[0065] Amplification reagents consist of components in the following volumes:

[0066]

[0067] The internal reference upstream primer is SEQ NO.1, the polymorphism primers are SEQ NO.2 and SEQ NO.3, and the downstream primer is SEQ NO.4.

[0068] A method for detecting the polymorphism of a speed or power-related gene-ACTN3, the steps comprising:

[0069] (1) DNA extraction of the sample genome;

[0070] (2) Detection and analysis of amplification and amplification products;

[0071] Wherein, PCR amplification reagent preferably is made up of the component of following volume:

[0072]

[0073] The internal reference upstream primer is SEQ NO.1, the polymorphism primers are SEQ NO.2 and SEQ NO.3, and the downstream primer is SEQ NO.4.

[0074] In addition, the PCR amplification reagent can also be selected from the above-mentioned embodiment, and its effect can also meet the detection requirements.

[0075] The PCR amplification procedure is as follows:

[0076] ...

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Abstract

The invention discloses a speed or explosive power relative gene-ACTN3 (Alpha-Actinin 3) polymorphism rapid detection kit. The speed or explosive power relative gene-ACTN3 polymorphism rapid detection kit comprises an amplification reagent and an amplified genetic typing reagent, wherein the amplification reagent comprises a PCR (Polymerase Chain Reaction) buffering solution, a dNTPs (Deoxyribonucleosidetriphosphate) mixture, a hot start Taq enzyme, a primer mixture and ultrapure water; the genetic typing reagent comprises reagent agarose, a TAE (Transcatheter Arterial Embolization) buffering solution, a DNA (Deoxyribose Nucleic Acid) molecular weight standard, a DNA non-poisonous dye and a DNA sampling buffering solution. The invention further discloses a speed or explosive power relative gene-ACTN3 polymorphism rapid detection method. The speed or explosive power relative gene-ACTN3 polymorphism rapid detection kit and the detection method have the beneficial effects that rapid reference is provided for material selection of sportsmen; the accuracy is high, the method is simple and easy to operate, the detection cost is low and the application and popularization are convenient.

Description

Technical field: [0001] The invention relates to the technical field of biological gene detection, in particular to a speed or power-related gene-ACTN3 polymorphism speed test kit and detection method. Background technique: [0002] In recent years, with the rapid development of molecular biology techniques and theories, restriction fragment length polymorphisms and single nucleotide polymorphisms have been widely used as genetic marker technologies, thus making it possible to search for genes that determine human athletic ability at the genetic level. It becomes possible and more scientifically and accurately evaluates the individual's sports status and sports potential, and also makes genetic selection the main research content of the scientific selection of future athletes. [0003] Studies have shown that the ACTN3 gene is related to muscle power and is a gene that guides the synthesis of α-actinin-3. It can be divided into three types: RR type, RX type and XX type. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q2531/113C12Q2535/125C12Q2565/125
Inventor 陈伟民张晓文
Owner GUANGZHOU INST OF SPORTS SCI
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