Preparation method of warburgia extract and application thereof

An extract, a technology of dozens of camphor, applied in the field of ethnic medicine, to achieve the effect of improving oral ulcers

Active Publication Date: 2014-02-05
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the preparation and characterization meth

Method used

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  • Preparation method of warburgia extract and application thereof
  • Preparation method of warburgia extract and application thereof
  • Preparation method of warburgia extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of preparation method of camphor extract, the steps are:

[0034]A. Take out the fresh Uganda ten dozen camphor bark frozen in the refrigerator at -78 or -79 or -80 or -81 or -82 ℃, and grind it to 60 or 70 or 80 or 90 or 100 mesh with a grinder.

[0035] B. Weigh the coarse powder of medicinal materials, pass through a 70 or 80 or 90 mesh sieve, add 200 mL each of methanol, n-hexane, ethyl acetate, ethanol and n-butanol at 10 times the volume (m / V), 38 or 39 or 40 Or ultrasonic treatment for 28 or 29 or 30 or 31 or 32 minutes under the condition of 41 or 42°C.

[0036] C. Centrifuge the extract at 10,000 r / min for 28 or 29 or 30 or 31 or 32 minutes to obtain a supernatant. Each solvent was repeatedly extracted 2 or 3 or 4 times respectively, and the supernatant was combined, and the organic solvent was removed by rotary evaporation at 44 or 45 or 46°C to obtain a concentrated extract.

[0037] D. The ethanol extract is freeze-dried or spray-dried to obtain an ...

Embodiment 2

[0040] A kind of preparation method of camphor extract, the steps are:

[0041] Take out the bark of fresh Uganda ten dozen camphor bark frozen in -80℃ refrigerator, and crush it with a grinder. Weigh 100.0 g of coarse medicinal powder, add 200 mL each of n-hexane, ethanol, methanol and n-butanol at 10 times the volume (m / V), and ultrasonicate for 30 min at 40 °C. The extract was centrifuged at 10000r / min for 30min to obtain the supernatant. Each solvent was extracted three times, and the supernatants were combined, and the organic solvent was removed by rotary evaporation at 45°C to obtain 9.7 g of ethanol phase extract. Take advantage of figure 2 As shown in the LC-ESI-MS detection, the main active ingredient is sesquiterpenoids.

[0042] Other steps are the same as in Example 1.

Embodiment 3

[0044] (1) Glycerin (8%, accounting for the total mass percentage of the paste, the same below), sorbitol (20%), polyethylene glycol (2%), sodium saccharin (0.3%), carboxymethyl cellulose Sodium benzoate (0.8%), sodium benzoate (0.2%), deionized water (31%) and other materials were put into an automated high-temperature (450°C) reactor, heated (75 or 80 or 86 or 90 or 95 or 100°C) and stirred to form Gel, cool (40 or 42 or 45 or 47 or 48 or 50 ℃) for use;

[0045] (2) Under the action of stirring, the prepared tens of camphor extract (3%), water and silica (10%), calcium hydrogen phosphate dihydrate (21%), tetrasodium pyrophosphate (0.2%), Sodium dihydrogen phosphate (0.1%), sodium lauryl sulfate (2.2%), natural spice mint (1.2%) and other raw materials are slowly added one by one, continue stirring to mix all kinds of materials evenly, and start cooling from 75-100 °C , slowly descend, and cool to 40 or 42 or 44 or 46 or 48 or 50°C.

[0046] (3) Cool the above materials (40...

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Abstract

The invention discloses a preparation method of warburgia extract and the application of the warburgia extract. The preparation method comprises the steps of A. taking out fresh Uganda warburgia bark which is cryopreserved in a refrigerator at the temperature of -78 to -82 DEG C, and grinding the Uganda warburgia bark into 60-100 meshes by a grinding mill; B. weighing medicine coarse powder, enabling the medicine coarse powder to pass through a sieve with 70-90 meshes, respectively adding 200mL of methyl alcohol, 200mL of n-hexane, 20mL of ethyl acetate, 200mL of ethanol and 200mL of n-butyl alcohol into the medicine coarse powder according to the ten times amount of volume, and carrying out ultrasonic treatment for 28-32min at the temperature of 38-42 DEG C; C. carrying out centrifugation on the extracting solution for 28-32min at the speed of 10000r/min to obtain a supernatant, repeatedly extracting every solvent for 2-4 times, blending the supernatant, and carrying out rotary evaporation at the temperature of 44-46 DEG C to enable organic solvent to be volatilized to obtain concentrated extract; D. carrying out freeze-drying or spray-drying on ethanol extract to obtain the Uganda warburgia extract. The method is easy as well as simple and convenient to operate, takes the fresh Uganda warburgia bark which is introduced by a botanical garden as raw material, and comprises the steps of solvent ultrasonic extraction, centrifugation, impurity removal, concentration, drying and the like after the fresh Uganda warburgia bark is simply ground; the warburgia extract which is prepared by extraction is very high in bacteriostatic activity.

Description

technical field [0001] The invention belongs to the field of ethnic medicines, and more specifically relates to a method for preparing an extract of Cinnamomum camphora, and at the same time, relates to the use of the extract of Cinnamomum in treating or preventing oral diseases. Background technique [0002] Common oral diseases such as dental caries, gingivitis, periodontitis, gingival abscess, loose teeth, etc. are closely related to oral pathogenic bacteria. Once a small wound appears in the oral cavity, the pathogenic bacteria can also reach other parts of the body along with the blood circulation, causing diseases such as arthritis and endocarditis; and because of the close relationship between the oral cavity and the maxillofacial region and the organs of the head Once the onset of the disease is often acute and severe, poor control can easily cause systemic complications. The use of chemical mouthwash is an important means to prevent and treat oral diseases. Althoug...

Claims

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Application Information

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IPC IPC(8): A61K8/97A61K8/49A61K36/185A61K31/365A61P1/02A61P31/02A61Q11/00A61K31/11A61K31/12
Inventor 郭明全王青锋朱洺志刘源振
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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