Unlock instant, AI-driven research and patent intelligence for your innovation.

Short peptide for promoting plants to take roots, sprout, grow and prolong storage period and application thereof

A storage period, plant technology, applied in the field of biotechnology agriculture, to achieve the effect of excellent storage period and prolonged growth

Active Publication Date: 2015-06-17
FUJIAN LANHAO BIOTECH
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, Nisin is a polypeptide composed of 34 amino acids, which has a strong effect on foods with high protein content, and short peptides with less than 10 amino acids are used to prolong the storage of seeds or fruits period, there are no literature reports

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Short peptide for promoting plants to take roots, sprout, grow and prolong storage period and application thereof
  • Short peptide for promoting plants to take roots, sprout, grow and prolong storage period and application thereof
  • Short peptide for promoting plants to take roots, sprout, grow and prolong storage period and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 Fruits: such as sugar cane:

[0058] Experimental effect of promoting sugarcane germination (synthesis of the short peptides involved in the present invention: Tyr-Leu-Tyr-Glu-Val-Gly-Arg and Tyr-Leu-Tyr-Glu-Val-Ala-Arg and their 4 interceptions in the sequence Amino acid: Glu-Val-Ala-Arg, truncated 5 amino acids: Tyr-Glu-Val-Ala-Arg and truncated 6 amino acids: Leu-Tyr-Glu-Val-Ala-Arg)

[0059] The short peptides Tyr-Leu-Tyr-Glu-Val-Gly-Arg, Tyr-Leu-Tyr-Glu-Val-Ala-Arg, Glu-Val-Ala-Arg, Tyr-Glu of the present invention were chemically synthesized by solid-phase Fmoc method -Val-Ala-Arg and Leu-Tyr-Glu-Val-Ala-Arg, after HPLC purification, the purity exceeds 98.4%, 97.8%, 95.9%, 99.6% and 96.3%, respectively dissolve the present invention with ultrapure water Peptides were prepared as 0.01, 0.05, 0.1, 0.5, and 1 mg / ml solutions, respectively. Germination experiments were carried out using fresh sugarcane.

[0060] Select MS basic medium, add 3% sucrose, a...

Embodiment 2

[0066] Example 2 Vegetables: leafy vegetables such as Chinese cabbage, spinach, celery, water spinach, etc.

[0067] The experimental effect of promoting the germination of Chinese cabbage, spinach, celery, and water spinach seeds (synthesis of the short peptide derivatives involved in the present invention, adding phosphoric acid group modification to tyrosine: Tyr p -Leu-Tyr-Glu-Val-Gly-Arg and Tyr p -Leu-Tyr p -Glu-Val-Gly-Arg)

[0068] The short peptide of the present invention is chemically synthesized by the solid-phase Fmoc method, and the R group of Tyr is replaced with a phosphoric acid group, replacing one or two groups. After HPLC purification, the purity exceeds 98%, and it is dissolved in ultrapure water The peptides of the present invention were prepared as 10 and 100 ug / ml solutions respectively.

[0069] Chinese cabbage: take Chinese cabbage seeds and place them at a temperature of 22°C and contain the short peptide (Tyr p -Leu-Tyr-Glu-Val-Gly-Arg) solut...

Embodiment 3

[0073] Example 3 Fruits and vegetables such as cucumbers, peppers, tomatoes, eggplants, beans, beets, etc.;

[0074] Promote the germination of fruits and vegetables such as cucumber, pepper, tomato, eggplant, beans, beet seeds (synthesis of the short peptide involved in the present invention: Tyr-Ile-Tyr-Glu-Val-Ala-Arg and its short peptide variants)

[0075] The short peptide Tyr-Ile-Tyr-Glu-Val-Ala-Arg of the present invention was chemically synthesized by the solid-phase Fmoc method. After HPLC purification, the purity exceeded 99%. The peptide of the present invention was dissolved in ultrapure water to prepare 100 ug / ml of solution.

[0076] Cucumber: Take cucumber seeds and place them in a solution containing the above-mentioned short peptides involved in the present invention at a temperature of 28 ℃ to 30 ℃, soak the seeds for 1 hour, take them out, wrap them in a damp cloth, and the germination time is about 8 hours, and new shoots will emerge , shortened the ge...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
elongationaaaaaaaaaa
Login to View More

Abstract

The invention provides a short peptide and application that promotes seed plant germination, growth and extending the storage period.The main sequence of the short peptide fragments, analogs, derivatives, and variants of this short peptide has the same as the same as the main sequence of the short peptide amino acid, ≥90%;Plane fragments, short -peptide analogs, short -peptide derivatives, and short -peptide variants are used to prepare functional products and applications that promote plant seeds rooting, germination, growth, and extending the storage period.The short -peptide molecular structure involved in the present invention is clear, the production technology is mature, and the finished products are non -toxic and harmless and no side effects. The significant feature is to promote the rooting, germination, growth, and extending the storage period of plant seeds.

Description

Technical field [0001] The present invention is a biotechnology agricultural field, and the invention also involves a short peptide and its application that promotes plant rooting, germination, growth, and extending the storage period. Background technique [0002] The rooting, germination, growth and storage of plants have always been the most critical issue of agricultural development.The substances that can promote the rapid root, germination, and growth of plant seeds are urgently needed for agricultural development today.At present, most of the plant growth in the market is botanical hormones, and the most important chemicals in the body are 3-acetic acid.Plant growth regulators with similar artificial synthetic effects include mossin, cell division, gibbererin, sodium acetate, amine fresh ester (DA-6), etc.This type of growth is duality, which can promote growth and inhibit growth.That is, low concentration promotes growth and high concentration suppress growth.Most of thes...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/315C12N15/31A01N47/44A01N37/46A01P21/00A23L3/3526C12R1/46
CPCC07K7/06
Inventor 李昊
Owner FUJIAN LANHAO BIOTECH