Narcissus BES-SSR marker primer TCA9 and method for identifying narcissus species

Abstract

The invention relates to a narcissus BES-SSR marker primer TCA9 and a method for identifying narcissus species. The method comprises the following steps: design and synthesis of the narcissus BES-SSR marker primer TCA9, extraction of total DNA (deoxyribonucleic acid) from a narcissus genome, establishment of a PCR (polymerase chain reaction) total reaction system, establishment of PCR amplification programs, establishment of polyacrylamide gel electrophoresis conditions, and detection and identification of PCR amplification products. A narcissus BES-SSR marker developed by a BAC-terminal sequence of the narcissus genome is adopted, has the characteristics of high accuracy, easiness in judgment, good repeatability, effect of not being affected by environmental factors, clear spectral band and the like, is an effective and reliable molecular marker, and further has the advantages of small sampling quantity, convenience in operation, reliable results and the like when used for identifying the narcissus species. The narcissus BES-SSR marker primer TCA9 can also be used for drawing of a narcissus species fingerprint spectrum, analysis of genetic diversity, research of a population genetic structure, analysis of a genetic linkage map and other technical fields.

Description

technical field [0001] The invention relates to a BES-SSR marker primer and a method for identifying a crop variety thereof, in particular to a narcissus BES-SSR marker primer TCA9 and a method for identifying a narcissus variety, belonging to the field of molecular biology. Background technique [0002] Narcissus is a genus of Amaryllidaceae Narcissus ( Narcissus L .) Perennial flower bulbs, most of which are native to the Mediterranean coast and central Europe. There are about 40 native species and many varieties. According to the statistics of the Royal Horticultural Society, there are now more than 10,000 horticultural varieties. It is very rich, and some daffodils have a pleasant fragrance, and have become famous ornamental and medicinal flowers widely cultivated in countries all over the world. However, narcissus has the characteristic of dormancy in summer and blooms once a year. It takes a long time to identify the flower type and color, which is time-consuming and ...

AI Technical Summary

Problems solved by technology

[0006] At present, the developed SSR markers mainly include G-SSR (genome SSR) and EST-SSR (expressed sequence tag SSR). However, there are still few narcissus EST sequences registered in the existing narcissus EST database, and the development of SSR markers is far away. Insufficient, and the polymorphism of SSR markers obtained by using EST sequences is lower than that of genomic SSR markers. Therefore, the information of SSR loci is obtained from the narcissus genome, and the SSR marker primers based on the narcissus genome are developed to identify narcissus germplasm and analyze genetic diversity. The field provides efficient labeling techniques that are imminent

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