Preparation method of heparin lithium

A technology of heparin lithium and heparin sodium, which is applied in the chemical industry, can solve the problems of high raw material cost, poor operability, and high price of heparin lithium, and achieve the effects of reducing production costs, wide sources of raw materials, and low preparation costs

Inactive Publication Date: 2014-02-26
ANHUI IND & TRADE VOCATIONAL TECHN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, heparin lithium is prepared by using heparin sodium as raw material through the exchange of sodium ions and lithium ions. This method needs to use high-quality heparin sodium as a raw material, and the high cost of raw materials makes heparin lithium expensive.
For example, Chinese patent 200510019846.0 discloses an ion balance exchange method to prepare heparin lithium. Part of the process of this method requires low temperature conditions (below 10°C) to operate, and the requirements for material and environmental temperature control are relatively high, and the investment in hardware facilities is large; The use of strong acidic conditions in the protein removal process will cause a large loss of heparin activity, affecting the final activity yield and titer, and the operability is not strong

Method used

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  • Preparation method of heparin lithium

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Embodiment 1

[0031] A preparation method of lithium heparin is as follows:

[0032] (1) Adsorption:

[0033] Weigh 500kg of porcine small intestine mucosa and place it in a reaction pot, add water to dilute, adjust the pH value to 9.0-9.5 with 30% sodium hydroxide solution, heat to boiling, keep for 15 minutes, cool down to 35°C, and at the same time, siphon the filtrate into the reaction tank, add 10% D204 resin resin, heat preservation and stirring for 10h;

[0034] (2) Washing:

[0035] Take the adsorbed liquid in step (1) and let it stand for 30 minutes, then filter it, collect the resin, rinse the resin with water, and drain it for later use;

[0036] (3) Elution:

[0037] Take the solid in step (2) and add 1mol / L sodium chloride to wash for 1~3h, after draining, add 4mol / L sodium chloride 1~3 times, stir and elute for 5h, drain and then elute twice, The amount of 4mol / L sodium chloride solution is halved each time, the elution time is 2h and 1h respectively, and all eluents are c...

Embodiment 2

[0048] A preparation method of lithium heparin is as follows:

[0049] (1) Adsorption:

[0050] Weigh 500kg of porcine small intestinal mucosa and put it in a reaction pot, add water to dilute, adjust the pH value to 9.0-9.5 with 30% sodium hydroxide solution, heat to boiling, keep for 15min, cool down to 35°C, and at the same time, siphon the filtrate into the reaction tank, add 10% D204 resin resin, heat preservation and stirring for 10h;

[0051] (2) Washing:

[0052] Take the adsorbed liquid in step (1) and let it stand for 30 minutes, then filter it, collect the resin, rinse the resin with water, and drain it for later use;

[0053] (3) Elution:

[0054] Take the solid in step (2) and add 1mol / L sodium chloride to wash for 1 to 3 hours. After draining, add 1 to 3 times the amount of 4mol / L sodium chloride, stir and elute for 5 hours, and then elute twice after draining. The amount of 4mol / L sodium chloride solution is halved each time, the elution time is 2h and 1h re...

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Abstract

The invention discloses a preparation method of lithium heparin, porcine small intestinal mucosa is used as a raw material for extracting a refined heparin sodium product, and ion exchange resin is used for realization of exchange of a heparin sodium salt and a heparin lithium salt; the preparation method comprises the following steps: preparation of the refined heparin sodium product, lithium exchange and freeze drying. According to the preparation method, after the porcine small intestinal mucosa is used as the raw material for extracting the refined heparin sodium product, the ion exchange resin is used for realization of the exchange of the heparin sodium salt and the heparin lithium salt; raw material sources are wide, and the preparation cost is low.

Description

technical field [0001] The invention belongs to the field of chemical industry and relates to a method for extracting heparin, in particular to a method for preparing lithium heparin. Background technique [0002] Lithium heparin has a unique value in clinical testing and is widely used in the collection and anticoagulation of blood samples for emergency biochemical examinations and hemorheological examinations. Since it generally takes more than an hour for blood to be coagulated and precipitated from the human body, it seriously affects the reporting time of emergency results. Therefore, heparin anticoagulated plasma is often used for biochemical examination in clinical emergency. When testing sodium ions in blood samples, in order to avoid affecting the test results, lithium heparin is often used instead. In addition, lithium heparin is an ideal anticoagulant for erythrocyte permeability test. [0003] At present, heparin lithium is prepared by using heparin sodium as a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
Inventor 方蕊
Owner ANHUI IND & TRADE VOCATIONAL TECHN COLLEGE
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