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Swine Mycoplasma Pneumonia Vaccine Strain

A technology of mycoplasma pneumoniae and mycoplasma hyopneumoniae, which is applied in the direction of viruses/bacteriophages, microorganisms, biochemical equipment and methods, etc., can solve the problems of affecting the growth and development of pigs and feed conversion rate, destroying the mucosa-cilia barrier, and lack of effective drugs, etc. , to achieve the effects of ensuring immune efficacy, reducing adverse reactions, and reducing dosage

Active Publication Date: 2015-08-19
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After Mhp infects the body, it mainly adheres to the cilia on the inner wall of the respiratory tract, causing cilia cell lesions and apoptosis, causing cilia to break and fall off, causing disease in the body, and will seriously affect the growth and feed conversion rate of pigs, or destroy the mucosa-cilia barrier. It is easy to cause secondary infection of other pathogenic bacteria (especially for young pigs), increasing the lethality rate, thus causing huge economic losses to the pig industry
At present, the main prevention and treatment measures of swine mycoplasma pneumonia are vaccines and drugs, but effective drugs are lacking. Therefore, the development of vaccines for this disease will be of great significance for the prevention and control of this disease

Method used

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  • Swine Mycoplasma Pneumonia Vaccine Strain
  • Swine Mycoplasma Pneumonia Vaccine Strain
  • Swine Mycoplasma Pneumonia Vaccine Strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: the breeding of Mycoplasma hyopneumoniae NJ strain

[0018] 1 strain isolation and culture

[0019] After the diseased lung tissue was ground, it was added to a liquid medium (pH 7.4-7.6), and cultured at 37°C for 3-10 days, then the pH dropped to 6.6-7.0, and it became slightly uniform and turbid. Then inoculate the solid medium and culture it at 37°C for 3 to 10 days, and fried egg-like colonies can be seen. After passage on the solid medium for 3 times, inoculate the liquid medium to obtain Mycoplasma hyopneumoniae.

[0020] 2 identification

[0021] The isolated Mycoplasma hyopneumoniae strains were cultured at 37°C for 3-10 days, and then the pH value dropped to 6.6-7.0, with slight uniform turbidity. The smear was carried out, and typical Mycoplasma hyopneumoniae cells were seen under the staining microscope. Then inoculate solid culture medium and culture at 37°C for 3-10 days, and fried egg-like colonies can be seen. Consistent with the culture ...

Embodiment 2

[0029] Example 2: The preparation method of the inactivated vaccine (NJ strain) of mycoplasma pneumoniae in swine.

[0030] 1 strain culture

[0031] Mycoplasma hyopneumoniae NJ strain was selected as the strain, which was isolated and identified by us and obtained after subculture. This strain was sent to the China Center for Type Culture Collection (CCTCC) on July 16, 2012, with the preservation number: CCTCCM2012286.

[0032] 2 strain characteristics

[0033] 2.1 Morphological and biochemical characteristics This bacterium is a Gram-negative polymorphic microorganism, which has ring, spherical, point, rod and bipolar shapes, is not easy to color, and has no cell wall. The biochemical characteristics should conform to the characteristics of the bacteria in bacterial taxonomy.

[0034] 2.2 Culture characteristics In the liquid culture medium (pH value 7.4-7.6), after cultured at 37°C for 3-7 days, the pH value will drop to 6.6-7.0, slightly uniform and turbid, and the titer...

Embodiment 3

[0059] Example 3: Observation of immune effect.

[0060] 1 vaccine

[0061] Mycoplasma pneumonia inactivated vaccine (NJ strain) oil adjuvant, Mycoplasma pneumonia inactivated vaccine (NJ strain) ISA11RVG, Mycoplasma pneumonia inactivated vaccine (NJ strain) Gel01ST, Mycoplasma pneumonia inactivated vaccine produced by a foreign company.

[0062] 2 strong poison for inspection

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Abstract

The invention discloses a vaccine strain for mycoplasma pneumonia of a swine. The classified name of the vaccine strain is Mycoplasma hyopneumoniae, wherein the number of the bacterial strain is NJ, the vaccine strain is preserved in China Center for Type Culture Collection with the preservation number: CCTCC NO: M2012286, and the preserving date is 16th, July, 2012. The vaccine strain for mycoplasma pneumonia of the swine disclosed by the invention has certain pathogenicity to swine and good immunogenicity.

Description

technical field [0001] The invention relates to a vaccine strain of swine mycoplasma pneumoniae, which belongs to the field of veterinary biological products. technical background [0002] Mycoplasma hyopneumoniae (Mhp) is the main pathogen that causes Mycoplasma pneumoniae of swine (Mps, also known as swine asthma), and is also an important primary pathogen of porcine respiratory disease syndrome. Mhp mainly infects the respiratory tract of pigs, and its pathogenicity is not strong. The clinical symptoms are mainly cough and wheezing. Meat sample" consolidation. After Mhp infects the body, it mainly adheres to the cilia on the inner wall of the respiratory tract, causing cilia cell lesions and apoptosis, causing cilia to break and fall off, causing disease in the body, and will seriously affect the growth and feed conversion rate of pigs, or destroy the mucosa-cilia barrier. It is easy to cause secondary infection of other pathogenic bacteria (especially for young pigs) a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00C12R1/93
Inventor 刘茂军邵国青
Owner JIANGSU ACAD OF AGRI SCI
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