Using amRNAs to obtain a safe and alternative way to kill transgenic rice

A technology of genetically modified rice and extermination, applied in the field of genetic engineering
CN103602705BInactive Publication Date: 2016-02-03ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2016-02-03
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The invention discloses a method of using amiRNAs to obtain safe and selective killing of transgenic rice: while expressing exogenous genes, endogenous miRNAs are used as precursors to selectively inhibit the expression of herbicide detoxification enzymes in rice, thereby obtaining safe, Genetically modified rice with optional kill. Specifically: the exogenous gene expression frame and the amiRNAs expression frame for directional inhibition of detoxification enzymes are constructed on the same plant expression vector; The sub-functionality is connected. The invention obtains a method for safely selecting and eradicating transgenic rice by directional silencing the expression of enzyme genes involved in herbicide detoxification and detoxification metabolism in rice.
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Description

technical field

[0001] The invention relates to a method for using amiRNAs to obtain safe and selectively killed transgenic rice, and the used genes and plasmids belong to the technical field of genetic engineering. Background technique

[0002] Genetically modified crops have been widely promoted in many countries in the world, and my country is also vigorously developing and promoting transgenic technology and research. Currently, the use of transgenic crops as bioreactors to produce medicinal proteins, industrial enzymes and other research work is also in full swing (Larrick and Thomas, Current Opinion in Biotechnology, 2001, 12: 411-418). The planting of these genetically modified crops has the possibility of being mixed with non-genetically modified crops, resulting in important health and safety hazards and ecological safety hazards such as transgenic drift.

[0003] Cytochrome P450 is a kind of heme protein that uses the electrons delivered by NADPH to catalyze the a...

Claims

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