A method of using lactic acid bacteria to biosynthesize nano-zinc oxide and nano-zinc oxide compound feed additive
A nano-zinc oxide and biosynthesis technology, which is applied in the field of microbial fermentation and nanotechnology, can solve problems such as unseen technical reports, achieve the effects of reducing pollution, improving intestinal health, and increasing the utilization rate of nutrients
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0048] The preparation method of the nano-zinc oxide product biosynthesized by the highly zinc-resistant Lactobacillus salivarius L3 obtained after screening domestication from the weaned suckling pig feces in June 2011 by the inventor comprises the following steps: the high zinc-resistant Lactobacillus salivarius L3 The strain was deposited in the China Center for Type Culture Collection on April 8, 2013, and the preservation number is CCTCCM2013126.
[0049] S1. Fermentation culture of Lactobacillus. The seed medium and fermentation medium are MRS liquid medium, the pH value is 6.2-6.4, sterilized by moist heat at 121°C for 20-30min, and cooled to 37°C for later use. After activating the glycerol strains for 12 to 16 hours, insert the fermentation medium with an inoculum of 3% to 5% (V / V). After 10 hours of cultivation, add an equal volume of fresh medium to continue the cultivation for 8 hours, and the number of cells reaches 10. 9 CFU / ml, to obtain the fermentation broth;...
Embodiment 2
[0057] Utilize Lactobacillus plantarum (purchased from China Industrial Microorganism Culture Collection Management Center) to prepare nano-zinc oxide steps as follows:
[0058] S1. Fermentation and cultivation of Lactobacillus plantarum. After activating the glycerol strains for 16 to 24 hours, insert the fermentation medium with an inoculum of 3% to 5% V / V, add the same amount of fresh medium after 14 hours of cultivation and continue to cultivate for 12 hours, and the number of cells reaches 10 9 CFU / ml.
[0059] S2. Slowly add an equal amount of 0.35MZnCl to the fermentation broth while stirring 2 solution.
[0060] S3. After adjusting the pH value of the mixture to 6.0, place it in an 80° C. water bath and heat it for 25 minutes to incubate.
[0061] S4. After hatching, put them into a 37° C. incubator to age for 9 hours.
[0062] S5. After aging, the cells and nano-zinc oxide precipitates are collected by centrifugation.
[0063] S6. The centrifuged product is calci...
Embodiment 3
[0066] Utilize Lactobacillus plantarum (purchased from China Industrial Microorganism Culture Collection Management Center) to prepare nano-zinc oxide steps as follows:
[0067] S1. Fermentation and cultivation of Lactobacillus plantarum. After activating the glycerol strains for 16 to 24 hours, insert the fermentation medium with an inoculum of 3% to 5% V / V, add the same amount of fresh medium after 14 hours of cultivation and continue to cultivate for 12 hours, and the number of cells reaches 10 9 CFU / ml.
[0068] S2. Slowly add an equal amount of 0.35MZnSO to the fermentation broth while stirring 4 liquid.
[0069] S3. After adjusting the pH value of the mixture to 6.0, place it in an 80° C. water bath and heat it for 25 minutes to incubate.
[0070] S4. After hatching, put them into a 37° C. incubator to age for 9 hours.
[0071] S5. After aging, the cells and nano-zinc oxide precipitates are collected by centrifugation.
[0072] S6. The centrifuged product was dried ...
PUM
| Property | Measurement | Unit |
|---|---|---|
| particle diameter | aaaaa | aaaaa |
| thickness | aaaaa | aaaaa |
| particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More