Screening method of positive substances for pyrolyzate trapping substance cytotoxicity test

A technology of cytotoxicity and pyrolysis, applied in the biological field, can solve the problems of undiscovered public reports, etc., and achieve the effect of strong operability and simple screening method

Active Publication Date: 2014-02-26
YUNNAN RES INST OF TOBACCO SCI
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Problems solved by technology

[0007] According to the method for screening positive substances in the cytotoxicity test of pyrolyzed product traps of the present

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  • Screening method of positive substances for pyrolyzate trapping substance cytotoxicity test

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Embodiment

[0028] In this embodiment, the screening of positive substances for the cytotoxicity test of pyrolyzed product traps of cigarette paper and its additives is described as an example.

[0029] (1) The results of the data query show that the maximum burning temperature of the cigarette paper layer is 420 °C. According to the principle of maximizing risk estimation, the maximum temperature of the cracking test is set to 450 °C in the cracking test method.

[0030] (2) Through data inquiry, four commonly used positive substances in cytotoxicity tests (sodium dodecyl sulfonate, malachite green, chloroquine phosphate and phenol) were used as the cytotoxicity test of cigarette paper and its additive pyrolysis product capture Alternative substances with positives. Query the physical and chemical properties of the four candidate substances (sodium dodecyl sulfonate, malachite green, chloroquine phosphate, and phenol) such as melting point, boiling point, and thermal stability.

[0031]...

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Abstract

The invention relates to a screening method of positive substances for a pyrolyzate trapping substance cytotoxicity test. The screening method comprises the following steps of: determining thermal cracking temperature according to the combustion state of substances participating in combustion; selecting some commonly used positive substances used for the cytotoxicity test as alternative substances of the positive substances used for the pyrolyzate trapping substance cytotoxicity test; determining whether to enter the follow-up screening step according to the boiling point, melting point, heat stability and other physicochemical properties of the alternative substances; performing the heat cracking test for the substances of which relevant physicochemical properties are failed to be inquired or the substances which have the boiling point below the combustion temperature and are not decomposed or decomposed a little during combustion; enriching the pyrolyzate; separating and analyzing through a gas chromatography-mass spectrometer; performing the heat cracking test for the substances failed to be decomposed or decomposed a little under the combustion temperature; performing MTT (Methyl Thiazolyl Tetrazolium) cytotoxicity test for the trapped pyrolyzate; obtaining the substances with cell inhibition not less than 80% to be used as the positive substances for the pyrolyzate trapping substance cytotoxicity test. By adopting the method, the positive substances for the pyrolyzate trapping substance cytotoxicity test can be screened effectively.

Description

[0001] technical field [0002] The invention relates to a screening method for positive substances used in a cytotoxicity test of pyrolysis product traps, belonging to the field of biotechnology. Background technique [0003] Pyrolysis technology is a technology that uses thermal energy to decompose macromolecular compounds into some compounds with relatively small molecular weight and relatively simple molecular structure. By using chromatographic technology to analyze the composition and structure of these small molecular compounds, the cracked products are qualitatively analyzed. And quantitative analysis, you can study the relationship between the composition, structure and physical and chemical properties of the cracked product and the original sample, as well as the cracking mechanism and reaction kinetics. As a special consumer product, cigarettes are consumed through the generation of smoke after combustion, while pyrolysis technology can quickly raise the temperat...

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Application Information

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IPC IPC(8): G01N33/50
CPCG01N33/5014
Inventor 黄海涛夭建华米其利耿永勤朱洲海李雪梅高茜曾婉俐管莹周岚
Owner YUNNAN RES INST OF TOBACCO SCI
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