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Brick-red xerocomus silwoodensis test tube sporocarp primordium inducing method

A technology of boletus velvet cap and test tube, which is applied in the field of induction of fruiting body primordium of boletus velvet cap in test tube, and can solve the problem of slow growth of mycelium, incomplete understanding of the symbiotic mechanism of symbiotic tree species in mycelium growth and development, etc. problem, to achieve the effect of easy operation, increased cultivation cost, and low production cost

Inactive Publication Date: 2014-03-05
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The artificial cultivation of boletus, like other rare edible fungi, has many blind spots. On the one hand, mycelium grows very slowly on the medium; It is perfectly clear that so far there are no cultivated varieties

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0018] Pick mature fruiting bodies in the field that grow robustly, have no pests, have not fully opened umbrellas, have thicker stipe, and thicker caps; bring the fruiting bodies back to the laboratory, and use alcohol cotton balls on the ultra-clean table to wipe the surface of the caps lightly and the stipe part, use a scalpel blade to gently remove the epidermis in the middle of the cap, take a cross from the middle of the cap, divide the fruiting body into four, take the internal sterile tissue block at the junction of the stipe and the cap, and cut into 0.25cm 2 small pieces;

[0019] Gently embed the cut small tissue pieces on the surface of a 18×180 mm test tube slant induction medium, the medium components are: 80.00 g / L of potato, 35 g / L of enzymatically decomposed pine needle powder, 15 g of fermented corn compound powder / L, CaCl 2 1.00g / L, KH 2 PO 4 0.60g / L, 80ml / L of wort juice of 16.0 Baume, 12.00 g / L of agar, the control pH value is 5.40; The preparation me...

example 2

[0022] Pick mature fruiting bodies in the field that grow robustly, have no pests, have not fully opened umbrellas, have thicker stipe, and thicker caps; bring the fruiting bodies back to the laboratory, and use alcohol cotton balls on the ultra-clean table to wipe the surface of the caps lightly and the stipe part, use a scalpel blade to gently remove the epidermis in the middle of the cap, take a cross from the middle of the cap, divide the fruiting body into four, take the internal sterile tissue block at the junction of the stipe and the cap, and cut into 0.35cm 2 small pieces;

[0023] The cut small tissue pieces were gently embedded on the surface of a 18×180 mm test tube slant induction medium. The medium components were: potato 90.00 g / L, enzymatic pine needle powder 38 g / L, fermented corn compound powder 17 g / L L. CaCl 2 1.00g / L, KH 2 PO 4 0.60g / L, 85ml / L of wort juice of 16.0 Baume, 12.00 g / L of agar, the control pH value is 5.60; The preparation method of descri...

example 3

[0026]Pick mature fruiting bodies in the field that grow robustly, have no pests, have not fully opened umbrellas, have thicker stipe, and thicker caps; bring the fruiting bodies back to the laboratory, and use alcohol cotton balls on the ultra-clean table to wipe the surface of the caps lightly and the stipe part, use a scalpel blade to gently remove the epidermis in the middle of the cap, take a cross from the middle of the cap, divide the fruiting body into four, take the internal sterile tissue block at the junction of the stipe and the cap, and cut into 0.49cm 2 small pieces;

[0027] The cut small tissue pieces were gently embedded on the surface of a 18×180 mm test tube slant induction medium. The medium components were: potato 100 g / L, enzymatic pine needle powder 40 g / L, fermented corn compound powder 20 g / L. L. CaCl 2 1.00g / L, KH 2 PO 4 0.60g / L, 90ml / L of wort juice of 16.0 Baume, 12.00 g / L of agar, the control pH value is 5.40; The preparation method of describe...

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Abstract

The invention relates to a brick-red xerocomus silwoodensis test tube sporocarp primordium inducing method, and belongs to the technical field of wild edible fungus cultivation. Xerocomus silwoodensis belongs to famous and precious wild edible funguses and is one of main delicious funguses at markets in summer of Yunnan province. Because the xerocomus silwoodensis is pine tree ectotrophic mycorrhiza fungus, can not be cultivated artificially and is all naturally picked in the field, the price is high. In odder to guarantee whole-year supplying of the bolete and meet dining requirements of the public, the price can be lowered only by achieving artificial cultivation. But many blind point problems exist in artificial cultivation of the bolete, for instance, hypha grows very slowly on a culture medium, and the symbiotic mechanism between the bolete and symbiotic trees still can not be known completely and clearly. The method includes the technical tasks that small sporocarp tissue blocks picked in the field are used as material, direct induced differentiation is performed on sporocarp primordium capable of being fully developed on the test tube culture medium, and the method has the advantages of being direct, simple, low in manufacturing cost and the like and provides an important basis for large-scale indoor artificial cultivation.

Description

technical field [0001] The invention relates to a method for inducing the primordia of the test tube fruiting body of the boletus velvet-capped boletus, which belongs to the field of biotechnology, and specifically belongs to the field of indoor cultivation of wild edible fungi. Background technique [0002] Boletus belongs to Basidiomycota (Basidiomycota), Agaricomycetes (Agaricomycetes), Boletales (Boletales), Boletaceae (Boletaceae). root fungus. The genus of fungi is delicious and contains physiologically active substances such as anti-oxidation and anti-tumor, so it has high economic value and research prospects. At present, except for a few saprophytic species such as Dictyostelium phalaenopsis, most of them are still difficult to cultivate artificially, and commercially available fruiting bodies are completely dependent on wild harvesting. Yunnan is rich in natural resources, and there are many varieties of wild boletus. However, due to climate change and ecological...

Claims

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Application Information

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IPC IPC(8): A01G1/04A01H4/00
Inventor 李宗菊王鹏飞张曦予吴鹏冯辽辽赵昱左奎
Owner YUNNAN UNIV
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