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Method and device for cultivating high-concentration photosynthetic bacteria with low illumination

A photosynthetic bacteria, high-concentration technology, applied in the field of microorganisms, can solve the problems of high temperature sterilization of anode and cathode buffers, low concentration of photosynthetic bacteria, limitation of large-scale production and application, etc. The effect of reducing energy consumption

Inactive Publication Date: 2015-05-13
重庆绿色智能技术研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The patent with publication number CN101409355B discloses a photosynthetic microbial fuel cell, which can efficiently treat wastewater and produce electricity, but the concentration of photosynthetic bacteria obtained is low, only 280 million / mL, and the anode and cathode buffers require high temperature Sterilization, which limits its large-scale production and application

Method used

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  • Method and device for cultivating high-concentration photosynthetic bacteria with low illumination
  • Method and device for cultivating high-concentration photosynthetic bacteria with low illumination
  • Method and device for cultivating high-concentration photosynthetic bacteria with low illumination

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Embodiment 1

[0038] A device for cultivating high-concentration photosynthetic bacteria with low light intensity. The cultivation device includes a cathode chamber and an anode chamber. The anode chamber is made of transparent material. The specifications of the two chambers are both 5cm×5cm×5cm. The two chambers are separated by a cation exchange membrane. There is a silica gel pad between the cation exchange membrane and the two electrode chambers to keep the seal, each chamber is filled with 120mL solution, the cathode and anode chambers are respectively provided with a cathode and an anode, and are connected by an external resistance, the external resistance is set to 500Ω, the anode Carbon felt is used as the electrode, the specification is 4cm×4cm, and the cathode uses carbon rod as the electrode. Add photosynthetic bacteria culture medium to the anode compartment, its composition is: sodium acetate 3g / L, NH 4 Cl1g / L, MgSO 4 0.2g / L, K 2 HPO 4 0.2g / L, NaHCO 3 1g / L, yeast powder 0.5g / L, ...

Embodiment 2

[0043] The device for cultivating high-concentration photosynthetic bacteria with low light intensity is the same as that in Example 1. The difference is that the cathode compartment is an air cathode, the two electrode compartments are separated by an anion exchange membrane, the external resistance is set to 1000 Ω, and the anode compartment is filled with 120 mL of alcohol wastewater. :Chemical oxygen demand 10g / L, total volatile organic acid 6g / L, ammonia nitrogen 0.5g / L, pH7.2, cathode adopts 0.5mg / cm 2 The platinum-carrying carbon paper is used as the electrode, the specification is 4cm×4cm. The photosynthetic bacteria with an inoculation volume percentage of 8% is a mixture of Rhodobacter sphaeroides and Rhodopseudomonas palustris at a ratio of 1:1, and the bacterial solution concentration is about 3 billion cells / mL (in the logarithmic growth phase). It is cultured under the conditions of 20-30℃ and 300 lux illumination. During the culture, the voltage is regularly colle...

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Abstract

The invention discloses a method and a device for cultivating high-concentration photosynthetic bacteria with low illumination. The device comprises an anode chamber and a cathode chamber, wherein the anode chamber is made from a transparent material, and is separated from the cathode chamber through an ion exchange membrane; the anode chamber and the cathode chamber are respectively provided with an anode and a cathode; the anode and the cathode are connected through an external resistor; the ratio of surface area of the anode to effective capacity of the anode chamber is more than 0.06 / cm, and ratio of resistance of the external resistor to effective capacity of the anode chamber is 4-40 ohm / cm3; the anode chamber of the device is a fermentation tank; photosynthetic bacteria cultivated to a logarithmic phase are inoculated to the anode chamber at a volume percentage of 1-10%, and the anode chamber is sealed and is communicated to an external circuit to cultivate the bacteria at 20-37 DEG C with illumination of 50-500lux. The method disclosed by the invention can cultivate high-concentration photosynthetic bacteria under low inoculum size and low illumination, and can generate electric energy while reducing illumination energy consumption; and the method is wide in application prospect.

Description

Technical field [0001] The invention belongs to the field of microorganisms, and particularly relates to a device for cultivating high-concentration photosynthetic bacteria with low illumination and a method for cultivating high-concentration photosynthetic bacteria using the device. Background technique [0002] Photosynthetic bacteria (PSB for short) is the earliest prokaryote that appears on the earth, is ubiquitous in nature, and has a primitive light energy synthesis system. Photosynthetic bacteria can decompose organic matter and absorb ammonia nitrogen, hydrogen sulfide, and sub- Nitrate and other harmful substances, the protein content of its cell dry matter is as high as 60%, its protein amino acid composition is relatively complete, and the cell also contains a variety of vitamins, especially the B vitamins are extremely rich, Vb2, folic acid, pantothenic acid, biological The content of vitamins is also high, and it also contains a lot of carotenoids, coenzyme Q and oth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12N1/20H01M8/16C12R1/01
CPCC12M29/04C12M35/02C12M41/28C12N1/20H01M8/16Y02E60/50
Inventor 王兴祖任以伟巫忠胜田恩玲
Owner 重庆绿色智能技术研究院
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