Method for extracting camellia seed meal protein employing ultrasonic assisted enzymolysis

Abstract

The invention relates to a method for extracting camellia seed meal protein employing ultrasonic assisted enzymolysis. The method comprises the following steps: (1) enzymolysis: evenly mixing crushed camellia seed meal which is filtered by an 80-100-mesh sieve with water according to the ratio of solid to liquid of 1:(6-7), adjusting the pH to 7.25-7.30, adding composite plant hydrolase which is 0.04-0.05% of mass of the camellia seed meal, carrying out enzymolysis at 65-70 DEG C for 3-3.5 hours, carrying out enzyme deactivation after enzymolysis is finished, and then centrifuging to obtain camellia seed meal residues; (2) ultrasonic assisted alkaline extraction: evenly mixing the camellia seed meal residues in the previous step with proper water, adjusting the pH value to 10.0-10.5, carrying out alkaline extraction at 40-45 DEG C for 80-90 minutes, and carrying out centrifugal separation after alkaline extraction is finished so as to obtain supernate, wherein ultrasonic assistance is adopted in the alkaline extraction process, the power of the ultrasonic wave is 300-1000W, and the ultrasonic time is 20 minutes; (3) acid precipitation: adjusting the pH value of the supernate obtained in the step (2) to 4.6, standing at room temperature for 1-1.5 hours, then centrifuging to obtain sediments, and freeze-drying the sediments to obtain the camellia seed meal protein. The extraction technology disclosed by the invention is mild in condition; the prepared camellia seed meal protein is good in shape, and color and luster, and can be widely applied to a feed additive.

Description

technical field [0001] The invention relates to a protein extraction method, in particular to an ultrasonic-assisted enzymolysis method for extracting camellia oleifera seed meal protein. Background technique [0002] Camellia oleifera (Camellia oleifera) is an evergreen small tree belonging to the family Theaceae and the genus Camellia. It is an excellent woody oil plant in the southern hilly areas of my country. my country is a big tea-producing country, and the planting area of ​​camellia oleifera forests alone reaches 4 million hectares 2 , is the country with the most varieties of Camellia oleifera, the widest distribution and the highest yield of Camellia oleifera seeds in the world, with an annual output of about 549,800 tons of Camellia oleifera seeds, 200,000 tons of camellia oil, and an average annual output of 397,100 tons of Camellia oleifera by-product—Camellia oleifera meal . The protein content in Camellia oleifera seed meal is about 12%-15%, and its amino ac...

AI Technical Summary

Problems solved by technology

In the prior art, the camellia oleifera seed meal protein is mostly extracted by alkali-soluble acid precipitation method, but the extraction efficiency of this method is low, and the shape and color of the extracted camellia seed meal protein are poor, which is not conducive to the use of the camellia oleifera seed meal protein as a food additive.