Method for extracting camellia seed meal protein employing ultrasonic assisted enzymolysis

An ultrasonic-assisted extraction technology of camellia oleifera seed meal, which is applied in the field of protein extraction, can solve the problems of unfavorable use of camellia oleifera seed meal protein, low extraction efficiency, protein shape and poor color of camellia oleifera seed meal, and achieve good color, good extraction effect, The effect of high extraction rate

Active Publication Date: 2014-03-12
衢州刘家香食品有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the camellia oleifera seed meal protein is mostly extracted by alkali-soluble acid precipitation method, but the extraction efficiency of this method is low,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example 1:

[0023] The method for extracting camellia seed meal protein assisted by enzymolysis and ultrasound, the method comprises the following steps:

[0024] (1) Enzymatic hydrolysis: The crushed camellia seed meal powder, which has passed through an 80 mesh sieve, is mixed with water at a material-to-liquid ratio of 1:6, adjusted to pH 7.25, and added with a compound plant hydrolase of 0.04% of the camellia seed meal powder mass. Enzymatic hydrolysis at 65°C for 3.5 hours, after enzymatic hydrolysis, inactivate the enzyme at 85°C for 10 minutes, and then centrifuge at 3000 rpm for 15 minutes to obtain the oil tea seed meal residue;

[0025] (2) Ultrasonic-assisted alkaline extraction: The residue of camellia seed meal (calculated on a dry basis) obtained in step (1) is mixed with water at a mass ratio of 1:25, adjusted to pH 10.0, and alkaline extraction is carried out at 40°C for 90 minutes. The extraction process uses ultrasonic assistance, the ultrasonic power is 30...

Example Embodiment

[0027] Example 2:

[0028] The method for extracting camellia seed meal protein assisted by enzymolysis and ultrasound, the method comprises the following steps:

[0029] (1) Enzymatic hydrolysis: After crushed, the camellia seed meal powder passed through a 100-mesh sieve is mixed evenly with water according to the material-to-liquid ratio of 1:7, the pH value is adjusted to 7.30, and the compound plant hydrolase of the camellia seed meal powder mass 0.05% is added. Enzymatic hydrolysis at 70°C for 3 hours, after enzymatic hydrolysis, the enzyme was inactivated at 85°C for 15 minutes, and then centrifuged at 3200 rpm for 10 minutes to obtain the oil tea seed meal residue;

[0030] (2) Ultrasonic-assisted alkaline extraction: The residue of camellia seed meal (on a dry basis) obtained in step (1) is mixed with water at a mass ratio of 1:25, adjusted to pH 10.5, and alkaline extraction is carried out at 45°C for 80 minutes. The extraction process adopts ultrasonic assistance, the ul...

Example Embodiment

[0032] Example 3:

[0033] The method for extracting camellia seed meal protein assisted by enzymolysis and ultrasound, the method comprises the following steps:

[0034] (1) Enzymatic hydrolysis: After crushed, the camellia seed meal powder passed through a 100-mesh sieve is mixed with water according to the material-to-liquid ratio of 1:6, and the pH value is adjusted to 7.28, and the compound plant hydrolase of the camellia seed meal powder mass 0.048% is added. Enzymatic hydrolysis at 68°C for 3.25h, after enzymatic hydrolysis, inactivate the enzyme at 90°C for 5min, then centrifuge at 3000rpm for 20min to obtain the camellia seed meal residue;

[0035] (2) Ultrasonic-assisted alkaline extraction: The residue of camellia seed meal (calculated on a dry basis) obtained in step (1) is mixed with water at a mass ratio of 1:25, adjusted to pH 10.0, and alkaline extraction is carried out at 40°C for 80 minutes. The extraction process adopts ultrasonic assist, the ultrasonic power is ...

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Abstract

The invention relates to a method for extracting camellia seed meal protein employing ultrasonic assisted enzymolysis. The method comprises the following steps: (1) enzymolysis: evenly mixing crushed camellia seed meal which is filtered by an 80-100-mesh sieve with water according to the ratio of solid to liquid of 1:(6-7), adjusting the pH to 7.25-7.30, adding composite plant hydrolase which is 0.04-0.05% of mass of the camellia seed meal, carrying out enzymolysis at 65-70 DEG C for 3-3.5 hours, carrying out enzyme deactivation after enzymolysis is finished, and then centrifuging to obtain camellia seed meal residues; (2) ultrasonic assisted alkaline extraction: evenly mixing the camellia seed meal residues in the previous step with proper water, adjusting the pH value to 10.0-10.5, carrying out alkaline extraction at 40-45 DEG C for 80-90 minutes, and carrying out centrifugal separation after alkaline extraction is finished so as to obtain supernate, wherein ultrasonic assistance is adopted in the alkaline extraction process, the power of the ultrasonic wave is 300-1000W, and the ultrasonic time is 20 minutes; (3) acid precipitation: adjusting the pH value of the supernate obtained in the step (2) to 4.6, standing at room temperature for 1-1.5 hours, then centrifuging to obtain sediments, and freeze-drying the sediments to obtain the camellia seed meal protein. The extraction technology disclosed by the invention is mild in condition; the prepared camellia seed meal protein is good in shape, and color and luster, and can be widely applied to a feed additive.

Description

technical field [0001] The invention relates to a protein extraction method, in particular to an ultrasonic-assisted enzymolysis method for extracting camellia oleifera seed meal protein. Background technique [0002] Camellia oleifera (Camellia oleifera) is an evergreen small tree belonging to the family Theaceae and the genus Camellia. It is an excellent woody oil plant in the southern hilly areas of my country. my country is a big tea-producing country, and the planting area of ​​camellia oleifera forests alone reaches 4 million hectares 2 , is the country with the most varieties of Camellia oleifera, the widest distribution and the highest yield of Camellia oleifera seeds in the world, with an annual output of about 549,800 tons of Camellia oleifera seeds, 200,000 tons of camellia oil, and an average annual output of 397,100 tons of Camellia oleifera by-product—Camellia oleifera meal . The protein content in Camellia oleifera seed meal is about 12%-15%, and its amino ac...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K1/30
Inventor 何东平胡传荣陈哲刘京伟
Owner 衢州刘家香食品有限公司
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