A kind of solid-state fermented feed rich in Bacillus phenyllactic acid coagulans and its preparation

A technology of bacillus coagulans and solid-state fermentation, applied in animal feed, animal feed, application, etc., to achieve the effects of increasing production, improving meat quality of livestock and poultry, and increasing utilization value

Active Publication Date: 2015-12-30
INNER MONGOLIA SCI PLUS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obtain solid-state fermented feed after solid-state fermentation culture

Method used

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  • A kind of solid-state fermented feed rich in Bacillus phenyllactic acid coagulans and its preparation
  • A kind of solid-state fermented feed rich in Bacillus phenyllactic acid coagulans and its preparation
  • A kind of solid-state fermented feed rich in Bacillus phenyllactic acid coagulans and its preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1 strain activation

[0044] Pick the bacteria in the glycerol tube → culture on the plate for 24 hours → pick the bacteria, connect to the slope, culture for 24, and store in the refrigerator at 4°C.

[0045] 2 seed liquid culture

[0046] Pick 2 rings of inclined-plane bacteria, insert seed liquid, 37±1°C, 200r.min -1 Ventilate culture, transfer to solid medium after 18h.

[0047] 3 solid state fermentation

[0048] 1) Composition of solid medium: water 500g / kg, malt extract powder 10g / kg, soybean meal 200g / kg, rice bran 200g / kg, mix evenly and pack, sterilize at 115°C for 20min.

[0049] 2) Cultivation of solid medium: add 5% of the weight of the solid medium to the seed solution, cultivate at 37±1°C, with a relative humidity of about 80%, at 0, 3, 6, 12, 24, 36, 48, 60 , 72h sampling.

[0050] 3) Determine the number of bacteria and pH changes at different times: put the samples taken at different times into the Erlenmeyer flask (the Erlenmeyer flask is equipped ...

Embodiment 2

[0056] 1 seed liquid culture

[0057] Pick 2 rings of slant bacteria, insert into the seed solution, 37°C, 200r.min -1 Ventilate culture, transfer to solid medium after 18h.

[0058] 2 solid state fermentation

[0059] 1) The composition of the solid-state fermentation medium: the amount of water added in the solid-state medium is 500g / kg, 550g / kg, 600g / kg, and 700g / kg; 200g / kg, 200g / kg, after mixing, sterilize at 115°C for 20min.

[0060] 2) Solid-state fermentation: insert seed solution according to 5% of the weight of the solid medium, cultivate at 37±1°C, relative humidity about 80%, and take a sample for 60 hours; mix the sample with normal saline in a ratio of 1:4, and put it in a refrigerator at 4°C After 4 hours, the supernatant was extracted and centrifuged to pass through the membrane.

[0061] 3 Measurement of phenyllactic acid content

[0062] Liquid phase conditions for the detection of phenyllactic acid: Agilent ZorbaxSB-C18 chromatographic column, mobile ph...

Embodiment 3

[0065] 1 seed liquid culture

[0066] Pick 2 rings of slant bacteria, insert into the seed solution, 37°C, 200r.min -1 Ventilate culture, transfer to solid medium after 18h.

[0067] 2 solid state fermentation

[0068] 1) The composition of the solid-state fermentation medium: the amount of water added to the solid-state fermentation medium is 600g / kg, the amount of malt extract powder is 10g / kg, the amount of soybean meal and rice bran are respectively 150g / kg, 150g / kg; 200g / kg, 100g / kg; 100g / kg, 200g / kg; 225g / kg, 75g / kg; 75g / kg, 225g / kg.

[0069] 2) Solid-state fermentation: add 5% of the weight of the solid medium into the seed solution, cultivate at 37±1°C with a relative humidity of about 80%, and take a sample for 60 hours; mix the sample with normal saline at a ratio of 1:4 and put it in a refrigerator at 4°C for 4 hours. Extract the supernatant, centrifuge through the membrane.

[0070] 3 Determination of phenyllactic acid content

[0071] Liquid phase conditions ...

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Abstract

The invention relates to production of bacillus coagulans solid-state fermentation feed rich in phenyllactic acid by utilization of a solid state fermentation technology. The bacillus coagulans is utilized to perform solid state fermentation. The yield of the phenyllactic acid in a sample is determined by controlling a fermentation process, extracting the phenyllactic acid in the late period of fermentation and exploring detection conditions. Finally, the bacillus coagulans solid-state fermentation feed having the highest yield of the phenyllactic acid is obtained by optimizing culture media and adding substrates. The feed effectively increases the laying rate of laying hens and reduces the death and culling rate and the feed-egg ratio. The optimum using concentration is 0.15%. The phenyllactic acid is a novel antibacterial substance, and has functions of improving animal immunity, improving livestock and poultry meat quality, reducing the quantity of escherichia coli in the livestock, and the like. Because of the production of the phenyllactic acid in the solid fermentation, the bacillus coagulans can give full play of probiotic functions, the utilization value of the solid fermentation feed is further increased, and the bacillus coagulans solid state fermentation feed rich in the phenyllactic acid has higher application value in the livestock and poultry breeding industry.

Description

technical field [0001] The invention belongs to the field of biological preparations, and relates to a preparation method for increasing the output of phenyllactic acid by solid-state fermentation of Bacillus coagulans, thereby obtaining solid-state fermented feed rich in phenyllactic acid, and obtaining a solid-state preparation with higher application value. Background technique [0002] Microecological preparations, also known as live bacterial preparations or probiotics, are mainly used to regulate the microecological balance of animals. They can prevent diseases directly by enhancing animals' inhibition of intestinal harmful microorganisms or by enhancing non-specific immune functions, thereby promoting A class of drugs or feed additives used for animal growth or to improve feed conversion. Microecological preparations have the characteristics of no toxic side effects, no drug resistance, no residue, low cost, significant effect, and no environmental pollution. They can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/14
Inventor 王海宽路福平张会图高雪芹
Owner INNER MONGOLIA SCI PLUS BIOTECH
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