Dominant microflora for degrading deca-brominated diphenyl ether waste water and preparation method thereof

A decabromodiphenyl ether, dominant flora technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc.

Inactive Publication Date: 2014-03-19
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of the economy, the composition of wastewater is becoming more and more complex, especially when the wastewater contains toxic and refractory organic pollutants, due to the small number and types of microorganisms with special degradation ability for this type of organic substances in the environment, and it At a disadvantage in interspecies competition, traditional biological treatment technologies face great challenges

Method used

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  • Dominant microflora for degrading deca-brominated diphenyl ether waste water and preparation method thereof
  • Dominant microflora for degrading deca-brominated diphenyl ether waste water and preparation method thereof
  • Dominant microflora for degrading deca-brominated diphenyl ether waste water and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025]Pick four rings of bacteria respectively: Agrobacterium sp., Bacillus sp., Gordonia, Pseudomonas putida (from soil polluted by electronic waste Separated from ) were transferred to 20mL nutrient solution containing 1.5g / L beef extract, 1.0g / L glucose, 5.5g / L tryptone, 3.0g / L yeast powder, pH6.0, and the rest In a container of water), each type of bacteria was cultured at 35°C for 1 day, and then the cultured four kinds of bacteria were inoculated to 300mL sterile Cultured in the container of the proliferation medium for 2 days under the condition of 27° C., centrifuged at 6000 rpm for 15 minutes, and obtained the logarithmic growth phase cells of the above four kinds of bacteria respectively. Among them, four different bacteria use different proliferation media, Agrobacterium sp. and Bacillus sp. use nutrient broth, the composition of which is as follows: beef extract 3.0g / L, tryptone 4.0g / L, the rest is water; Gordonia (Gordonia) adopts the yeast extract glucose mediu...

Embodiment 2

[0031] Four rings of bacteria were picked respectively: Agrobacterium sp., Bacillus sp., Gordonia, Pseudomonas putida (from soil polluted by electronic waste Separated from) were transferred to 40mL nutrient solution containing 2.0g / L beef extract, 1.5g / L glucose, 6.5g / L tryptone, 3.5g / L yeast powder, pH7.0, and the rest In a container of water), each type of bacteria was cultured at 30°C for 1 day, and then the cultured four kinds of bacteria were inoculated at a volume ratio of 1:10 (mixed bacteria solution and nutrient solution) to contain 400mL of no Cultivate in a container of bacterial proliferation medium for 1 day at 35° C., centrifuge at a speed of 7000 rpm for 10 min, and obtain logarithmic growth phase cells of the above-mentioned four kinds of bacteria respectively. Among them, four different bacteria use different proliferation media, Agrobacterium sp. and Bacillus sp. use nutrient broth, the composition of which is as follows: beef extract 3.5g / L, tryptone 5.0g ...

Embodiment 3

[0037] Four rings of bacteria were picked respectively: Agrobacterium sp., Bacillus sp., Gordonia, Pseudomonas putida (from soil polluted by electronic waste Separated from) were transferred to 50mL nutrient solution (the main components are 1.7g / L beef extract, 2.0g / L glucose, 6.0g / L tryptone, 4.0g / L yeast powder, pH8.0, The rest is water), each kind of bacteria was cultured at 27°C for 2 days, and then the cultured four kinds of bacteria were respectively inoculated with a volume ratio of 1:12 (mixed bacteria solution and nutrient solution) to contain 500mL Cultivate in a sterile proliferation medium container for 1 day at 35° C., centrifuge at a speed of 6000 rpm for 15 minutes, and obtain logarithmic growth phase cells of the above four bacteria respectively. Among them, four different bacteria use different proliferation media, Agrobacterium sp. and Bacillus sp. use nutrient broth, the composition of which is as follows: beef extract 4.0g / L, tryptone 4.5g / L, the rest is...

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Abstract

The invention discloses a dominant microflora for degrading deca-brominated diphenyl ether waste water and a preparation method thereof. The preparation method comprises the following steps: respectively inoculating and culturing agrobacterium sp., bacillus sp., gordonia, and pseudomonas putida; mixing, by volume percent, 2-5% of agrobacterium sp., 10-15% of bacillus sp., 48-58% of gordonia, and 16-35% of pseudomonas putida to obtain the dominant microflora for degrading deca-brominated diphenyl ether waste water. During application, the dominant microflora is activated, mixed and put into waste water. The dominant microflora of the invention significantly improves the degradation efficiency for microorganism treating deca-brominated diphenyl ether waste water through synergistic effect of the bacteria.

Description

technical field [0001] The invention relates to the field of decabromodiphenyl ether wastewater treatment, in particular to a dominant bacterial group for degrading decabromodiphenyl ether wastewater and a preparation method thereof. Background technique [0002] Biological treatment is a commonly used wastewater treatment method at present. This method decomposes and absorbs the pollutants in wastewater through the metabolism of microorganisms, so as to achieve the purpose of pollution control. Compared with other methods, biological treatment has low cost, high efficiency, easy operation, and most importantly, no secondary pollution. Therefore, it has been widely used in wastewater treatment. With the development of the economy, the composition of wastewater is becoming more and more complex, especially when the wastewater contains toxic and refractory organic pollutants, due to the small number and types of microorganisms with special degradation ability for this type of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/40C12R1/01C02F101/38
Inventor 陈元彩吕源财胡勇有
Owner SOUTH CHINA UNIV OF TECH
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