Microbiological composite bacterial agent and application thereof in adjusting soil pH value
A technology of compound bacterial agents and microorganisms, applied in the direction of microorganism-based methods, microorganisms, applications, etc., can solve problems such as soil compaction, achieve the effects of increasing soil water retention, improving soil physical and chemical properties, and improving water and fertilizer retention capacity
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[0029] Preparation of microbial compound bacterial agent
[0030] 1. Transfer culture of strains
[0031] Bacillus subtilis (Bacillus subtilis) CICC10036 was inoculated in the Bacillus subtilis transfer medium with pH 7.0, and cultured at 30° C. for 24-48 hours. The composition of Bacillus subtilis (Bacillus subtilis) CICC10036 transfer medium is: bran 50.0g, bean cake powder 30.0g. After the above ingredients are mixed, add 1.0L of 0.2% NaOH solution, boil for 1 hour, neutralize to pH7 with HCI, then add 20.0g of agar, after melting, subpackage and sterilize.
[0032] Lactococcus lactis subsp. lactis CICC6030 was inoculated in CM0044 skim milk medium, and cultured at 30° C. for 48-72 hours. The composition of CM0044 skimmed milk medium is 120.0 g of skimmed milk, 1.0 L of distilled water, and natural pH. Sterilize at 113°C for 20 minutes.
[0033] Streptomyces microflavus (Streptomyces microflavus) ACCC40027 was inoculated in the following medium at pH 7.2-7.4 at a volume...
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