Microbiological composite bacterial agent and application thereof in adjusting soil pH value

A technology of compound bacterial agents and microorganisms, applied in the direction of microorganism-based methods, microorganisms, applications, etc., can solve problems such as soil compaction, achieve the effects of increasing soil water retention, improving soil physical and chemical properties, and improving water and fertilizer retention capacity

Active Publication Date: 2014-03-19
北京市京圃园生物工程有限公司
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AI Technical Summary

Problems solved by technology

Inorganic soil conditioners such as quicklime are effective in adjusting soil pH in a short period of time, but long-term application will cause problems such as soil compaction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation of microbial compound bacterial agent

[0030] 1. Transfer culture of strains

[0031] Bacillus subtilis (Bacillus subtilis) CICC10036 was inoculated in the Bacillus subtilis transfer medium with pH 7.0, and cultured at 30° C. for 24-48 hours. The composition of Bacillus subtilis (Bacillus subtilis) CICC10036 transfer medium is: bran 50.0g, bean cake powder 30.0g. After the above ingredients are mixed, add 1.0L of 0.2% NaOH solution, boil for 1 hour, neutralize to pH7 with HCI, then add 20.0g of agar, after melting, subpackage and sterilize.

[0032] Lactococcus lactis subsp. lactis CICC6030 was inoculated in CM0044 skim milk medium, and cultured at 30° C. for 48-72 hours. The composition of CM0044 skimmed milk medium is 120.0 g of skimmed milk, 1.0 L of distilled water, and natural pH. Sterilize at 113°C for 20 minutes.

[0033] Streptomyces microflavus (Streptomyces microflavus) ACCC40027 was inoculated in the following medium at pH 7.2-7.4 at a volume...

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PUM

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Abstract

The invention provides a microbiological composite bacterial agent and an application thereof in adjusting soil pH value. The bacterial agent comprises the following active components: bacillus, lactobacillus, saccharomycetes, soil actinomycetes, and mycete. The bacterial agent can adjust soil pH value, establishes a good crop rhizosphere micro-ecological environment, loosens the soil, increases the air permeability, improves the water-retention and fertilizer conservation performance of the soil, helps to form dominant bacterial communities of beneficial bacteria, effectively inhibits the growth of pathogenic microorganism, relieves the soil borne diseases, strengthens the anti-disease performance of crops, maintains the soil micro-ecological system and ecological balance, modulates the soil structure, improves the crop rhizosphere soil micro-ecological system, promotes the crop to grow roots, increases the crop output, improves the product quality, increases the microorganism content and trace element content in soil, and thus achieves the goals of improving physical and chemical properties of soil, strengthening the water-retention and fertilizer conservation performance of the soil, and increasing the air permeability of the soil. The microbiological composite bacterial agent has the advantages of user-friendliness, low cost, and prominent effect, can prevent the happening of insects and diseases, and improves the product output and quality.

Description

technical field [0001] The invention relates to a microbial composite bacterial agent and its application in regulating soil acidity and alkalinity. Background technique [0002] Soil is the basis for the development of agricultural production. Although the rapid development of modern agricultural technology has played a great role in increasing crop yield and improving quality, it is inseparable from the soil foundation. In recent years, due to the extensive use of chemical fertilizers in agricultural production, the input of traditional organic fertilizers has decreased, soil fertility has continued to decline, and physical and chemical properties have changed significantly. Moreover, due to factors such as human beings' unreasonable development and utilization of land and forest resources, and environmental pollution, soil erosion, land desertification, barrenness, and serious pollution have occurred. Therefore, soil improvement is one of the important tasks to protect s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/14C09K17/00C12R1/125C12R1/46C12R1/865C12R1/465C12R1/69C09K101/00C09K109/00
Inventor 张长现禹宙王永利赵海艳
Owner 北京市京圃园生物工程有限公司
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