Nicotine-degrading bacterium and application thereof

A technology of nicotine-degrading bacteria and tobacco stalks, which can be applied to bacteria, organic fertilizers, microorganisms, etc., and can solve the problems of lowering the quality of compost products.

Inactive Publication Date: 2014-03-19
湖北省烟草公司恩施州公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems in the prior art that microorganisms are inhibited by high-concentration nicotine in toba

Method used

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  • Nicotine-degrading bacterium and application thereof
  • Nicotine-degrading bacterium and application thereof
  • Nicotine-degrading bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: A nicotine-degrading bacterium of the present invention, which is taxonomically named Arthrobacter histidinolovorans (Arthrobacter histidinolovorans) EA-17, and was deposited in the China Type Culture Collection on September 15, 2013. The number is CCTCCM2013405. The industrial production of nicotine-degrading bacteria EA-17 is secondary fermentation. The production method is as follows: inoculate the activated bacteria in LB liquid medium, cultivate at 30-37°C for 10-18 hours, and prepare them into first-level seeds; 0.1%-0.5% of the inoculum is inoculated into 2M with liquid LB medium 3 In the fermentation tank, the liquid volume in the fermentation tank is 60%, 30-37℃, stirring speed 250rpm, aeration ratio 0.2VVM-1VVM, fermentation 16-24h, biomass up to 3×10 9 CFU / mL.

[0031] A kind of application of nicotine degrading bacteria in tobacco stalk piles. The use of nicotine degrading bacteria EA-17 is as follows: nicotine degrading bacteria fermentation broth a...

Embodiment 2

[0032] Example 2: Preparation of a nicotine-degrading bacterium EA-17 bacterial solution of the present invention

[0033] 1 medium

[0034] LB medium: peptone 10g, yeast powder 5g, sodium chloride 10g, agar 20g, distilled water 1000ml, pH7.0-pH7.2, 121℃, sterilized for 30min.

[0035] 2 Bacteria preparation

[0036] 2.1 Strain activation: Nicotine degrading bacteria EA-17 was inoculated on LB medium (2% agar added to LB medium), and cultured at 30°C for 18-24h.

[0037] 2.2 Primary strain culture: transfer the above-mentioned nicotine-degrading bacteria EA-17 to liquid LB medium, fill 50ml / 500ml Erlenmeyer flask, culture temperature 30℃, shaker speed 180r / min, shake Bed culture for 12h.

[0038] 2.3 Fermentation tank culture: Fermentation tank volume 2M 3 , 1200L medium, the above-mentioned primary seed inoculum is 2.4L, the stirring speed is 250rpm, the aeration ratio is 0.5VVM, the fermentation temperature is 30℃, and the fermentation is 20h.

[0039] 3 fermentation broth biomass

[00...

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Abstract

The invention relates to a nicotine-degrading bacterium and an application thereof. The nicotine-degrading bacterium is characterized in that the bacterium is named as Arthrobacter histidinolovorans EA-17 according to taxonomy and was collected in China Center for Type Culture Collection on September 15, 2013, and the collection number is CCTCC No. M2013405. A strain is fermented by a 2M3 fermentation tank for production, then mixed with a self-made decomposition agent according to the mass ratio of 1:10, further inoculated into a tobacco straw compost according to the inoculation amount of 1% and fermented for 30-45d, and then straw can be decomposed; compared with traditional compost fermentation, the nicotine degradation rate is improved by 60%-78%, the total nitrogen content is increased by 7%-9%, the total phosphorus content is increased by 0.3%-0.6%, and the total potassium content is increased by 6%-8%.

Description

Technical field [0001] The invention relates to a nicotine degrading bacteria and its application. Background technique [0002] In tobacco planting and production, a large amount of tobacco stalks, an agricultural waste, is produced. Tobacco stalks contain a high concentration of malignant pollutants nicotine, and carry pathogenic bacteria and insect eggs. Discarding or burning a large amount of tobacco stalks will cause serious soil and air pollution and aggravate the spread of soil-borne diseases. Therefore, reasonable treatment of tobacco stalks and utilization of waste tobacco stalks are the basis for the healthy development of tobacco planting. Tobacco stalks are rich in nutrients and organic matter required by plants such as N, P, K, etc., and high-temperature composting technology can use microbial degradation to convert tobacco stalks into high-quality organic fertilizers. In addition, the high-temperature environment of high-temperature composting can kill various pat...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/06
Inventor 陈守文呙亚屏王瑞冀志霞杨亮秦兴成伍良伟徐迪红施河丽
Owner 湖北省烟草公司恩施州公司
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