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A kind of fermentation medium of Bacillus thuringiensis bt20 bacterial strain

A fermentation medium and technology of thuringiensis spores, applied in the direction of fermentation, bacteria, and microorganism-based methods, can solve the problems of high cost of medium, unsuitability for large-scale production and popularization and application, etc., achieve simple fermentation process and enhance market competitiveness , enhance the effect of toxicity

Inactive Publication Date: 2015-10-28
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that the existing culture medium for Bt20 bacterial strains has high cost and is not suitable for industrialized large-scale production and popularization and application, and provides a fermentation medium for Bacillus thuringiensis Bt20 bacterial strains

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  • A kind of fermentation medium of Bacillus thuringiensis bt20 bacterial strain
  • A kind of fermentation medium of Bacillus thuringiensis bt20 bacterial strain
  • A kind of fermentation medium of Bacillus thuringiensis bt20 bacterial strain

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specific Embodiment approach 1

[0012] Specific embodiment one: the fermentation medium of a kind of Bacillus thuringiensis Bt20 bacterial strain of the present embodiment, by weight percentage, the component is: 1.5%~4% corn flour, 0.1%~3.5% fly maggot, 0.5% to 3.5% of soybean powder, 0.5% to 2.5% of yeast powder and the rest of water.

[0013] The strain preservation number of the Bacillus thuringiensis Bt20 strain described in this embodiment is: CGMCC NO.1710.

[0014] In this embodiment, fly maggot powder is selected as the nitrogen source of the fermentation medium for the first time to replace the original commonly used protein-fish meal, and it is found that the absorption and utilization of fly maggot powder by the Bacillus thuringiensis Bt20 strain is significantly better than other nitrogen sources, which improves the fermentation liquid. The absorbance value has enhanced the virulence of the strain, and can completely replace fish meal as the nitrogen source of the fermentation medium. This appli...

specific Embodiment approach 2

[0015] Specific embodiment two: the difference between this embodiment and specific embodiment one is that: the fermentation medium of the Bt20 bacterial strain, by weight percentage, consists of: 2% to 4% corn flour, 0.5% to 3% of fly maggots, 1% to 3% of soybean powder, 1% to 2% of yeast powder and the rest of water. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0016] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that: the fermentation medium of the Bt20 bacterial strain, by weight percentage, consists of: 2.5% to 3.5% corn flour, 1 1.5% to 3% of fly maggots, 1.5% to 3% of soybean powder, 1.5% to 2% of yeast powder and the rest of water. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to a fermentation medium for bacillus thuringiensis Bt20, which aims at solving the problem that the existing fermentation medium for bacillus thuringiensis Bt20 has high cost and is not suitable for industrial production and popularization and application in large scale. The best fermentation formula of the bacillus thuringiensis Bt20 comprises 3% of corn powder, 3.0% of fly maggot, 3% of soybean powder, 2% of yeast powder and the balance of water. By taking the absorbance value and insecticide toxicity test of the fermentation liquor as the index, carbon and nitrogen source of the fermentation formula is optimized through single factor and orthogonal test; by adopting the fermentation culture liquor fermented for 72 hours by the fermentation medium for the Bt20, the corrected mortality of juvenile of diamond back moth reaches 96.61%. The fermentation technology is simple, the raw materials are easy to obtain, the production cost is low, and the fermentation medium has strong market competitiveness and is applied to the filed of Bt cultivation.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a liquid fermentation medium formula of Bacillus thuringiensis Bt20 strain. Background technique [0002] With the improvement of people's awareness of environmental protection, biopesticides have attracted more and more attention. Among them, the Bt insecticide produced by fermentation of Bacillus thuringiensis (Bt for short) is currently the most widely used microbial insecticide, but the raw material cost of Bt insecticide is increasing year by year, especially with the global market The increase in the price of protein raw materials has significantly increased the fermentation cost of Bacillus thuringiensis, thereby affecting its market share. [0003] Before the commercial production of biocontrol agents, the formulation selection of the fermentation medium is particularly important. An effective fermentation medium formulation can produce enough i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P21/02C12R1/07
Inventor 赵奎军韩岚岚邢亚楠于洪春周长梅朱明贺韩晓旭
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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