Culture method for tissue culture seedlings of nitraria tangutorum
A culture method and tissue culture seedling technology, which is applied in the field of Nitraria tissue culture seedling cultivation, can solve the problems of explant damage, explant lethality, disinfection failure, etc., and achieve stable genetic traits, cost and operation simplification, Cost and Operational Savings Effect
Inactive Publication Date: 2014-03-26
甘肃省农业科学院生物技术研究所
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Problems solved by technology
Mercuric chloride can cause great damage to explants, and if the time is slightly improper, it will cause disinfection failure or serious death of explants, which will cause difficulties in later work
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment 1
[0025] (1) Test material: wild Nitraria;
[0026] (2) Disinfection of explants: cut the young stems from the seedlings obtained by germination, and sterilize them with 75% alcohol for 30 seconds, then use two disinfectants to treat the stems at different times for comparison, 0.1% chlorine Mercury treatment for 4, 6, 8, 10 minutes; 10% sodium hypochlorite treatment for 7, 10, 12, 15, 18 minutes, rinse with sterile water 4-5 times, connect to MS medium (tap water configuration), 10 bottles per treatment , 3 explants per bottle, cultured under the conditions of temperature 25±1°C, light intensity 2000Lx, and light 16h / d, and counted the disinfection status after a few days;
[0027] (3) Stem segment induction: The stem segment of Nitraria to be sterilized is rooted on MS medium (tap water configuration) and the joint grows to a height of 4-5cm, then the single-bud stem segment can be cut and cultivated in two ways: 1. The second one is connected to the differentiation medium fo...
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The invention discloses a culture method for tissue culture seedlings of nitraria tangutorum, provides a set of simple, economical and fast culture method for the tissue culture seedlings of the nitraria tangutorum, and the method is established by main steps that a nitraria tangutorum seedling after seed germination is disinfected with a suitable method and is subjected to direct rooting, growing and rapid propagation by a single bud stem on a simple medium. According to the method, firstly, a potion and a method suitable for disinfecting the tender nitraria tangutorum seedling are found; then a mode that direct rooting and seedling growing are performed by single bud stem is adopted for multiplication, a complete plant can be obtained stably in a short time, and the plant has green leaves and strong root and grows healthily; and finally, the medium is configured by tap water instead of distilled water, white sugar instead of sucrose is added as a carbon source, meanwhile, use of excessively additional plant growth regulators is avoided, so that the cost and operation are saved and simplified greatly, a novel practical path is provided for rapid propagation of the nitraria tangutorum, and foundation is laid for late industrialized production.
Description
technical field [0001] The invention relates to the field of group cultivation, in particular to a method for cultivating Nitraria tissue culture seedlings. Background technique [0002] Nitraria, also known as sand cherry, commonly known as jujube, has a well-developed root system and has excellent characteristics of salt-alkali resistance, drought resistance, and sand-fixing and soil-improving. important role. Cynomorium, which is parasitized by the root of Nitraria thorn, is a traditional and precious warming and tonic medicinal material. In recent years, searching for new and potential medicinal and edible homologous plants from wild plant resources has become a research focus of scholars at home and abroad, and the sand xerophyte Nitraria is one of the winners preserved after long-term natural screening. First, Nitraria is loved by people in sandy areas because of its tenacious vitality and excellent genetics. Whitethorn fruit contains a variety of nutrients and rich...
Claims
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IPC IPC(8): A01H4/00
Inventor 张艳萍赵玮罗俊杰陈玉梁罗万银董治宝
Owner 甘肃省农业科学院生物技术研究所